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MSCV retrovirus system

The MSCV Retroviral Expression System (murine stem csell virus) contains vectors that are optimized for introducing and expressing target genes in pluripotent cell lines, including murine or human hematopoietic, embryonic stem (ES), and embryonal carcinoma (EC) cells. They can also be used effectively with most mammalian cell lines. This highly efficient system is ideal for analyzing gene function in development, embryogenesis, or immune response, in both cell culture and transgenic assays.

The MSCV Retroviral Expression System (murine stem sell virus) contains vectors that are optimized for introducing and expressing target genes in pluripotent cell lines, including murine or human hematopoietic, embryonic stem (ES), and embryonal carcinoma (EC) cells. They can also be used effectively with most mammalian cell lines. This highly efficient system is ideal for analyzing gene function in development, embryogenesis, or immune response, in both cell culture and transgenic assays.

Designed for expression in hematopoietic cells and embryonic stem cells

The MSCV system contains three vectors: pMSCVneo, pMSCVhyg, and pMSCVpuro. These vectors contain a specially designed long terminal repeat (LTR) from the murine stem cell PCMV virus. This LTR differs from the MMLV LTR by several point mutations and a deletion that together enhance transcriptional activation and prevent transcriptional suppression in ES and EC cells. As a result, the PCMV LTR drives high-level, constitutive expression of the target gene in stem cells or other mammalian cell lines.

Packaging cell line included

The MSCV system includes the RetroPack PT67 Packaging Cell Line, which produces high-titer virus able to infect a broad range of mammalian host cells.

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634401

MSCV Retroviral Expression System

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License Statement

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These products are sold under license from the Fred Hutchinson Cancer Research Center. Rights to use this product are limited to research only. No other rights are conveyed. Inquiry into the availability of a license to broader rights or the use of this product for commercial purposes should be directed to Fred Hutchinson Cancer Research Center, Technology Transfer Office, 1100 Fairview Avenue North, C2M-027, Seattle, WA 98109. Purchase of this product does not grant rights to: (1) offer the materials or any derivatives thereof for resale; or (2) to distribute or transfer the materials or any derivatives thereof to third parties.

Use of the MSCV Retroviral Expression System is exclusively licensed from Robert G. Hawley, Ph.D. Academic research institutions are granted an automatic license with the purchase of this product to use the MSCV Retroviral Expression System only for internal, academic research purposes. This limitation expressly excludes the right to sell or otherwise transfer the MSCV system or its component parts to third parties. All other users and purchasers are required to obtain a license from Takara Bio USA, Inc. prior to purchasing these reagents or using them for any purpose. For information on licensing please contact a licensing representative by e-mail at licensing@takarabio.com.

The MSCV (Murine Stem Cell Virus) Retroviral Expression System contains 3 vectors that are optimized for the introduction and expression of genes in pluripotent cell lines, including murine or human hematopoietic, embryonic stem (ES), and embryonal carcinoma (EC) cells. They can also be used effectively with any mammalian cell line. After your gene of interest has been cloned into one of the vectors, the construct can be packaged into retroviral particles using the Retropack PT67 Packaging Cell Line (included).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

*You must be logged in to a Purchasing Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

Overview

Efficient long-term gene expression in ES and EC cells
Choice of three selectable markers
Complete retroviral system including a PT67 packaging cell line

More Information

Applications

Retroviral transduction of a broad range of cell types including embryonic stem cells and embryonic carcinoma cells

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Choose the right lentiviral product for your research

Lentiviral product selection guide

Takara Bio offers a broad range of research tools for gene delivery using lentiviral vectors, including a selection of vector systems, packaging mixes, and titration kits. Use our selection guide for a brief description of the products and links to further information.

Lentiviral products guide Learning center

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