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Recombinant RNase Inhibitor

Takara Bio's Recombinant RNase Inhibitor acts as a noncompetitive inhibitor of RNases A, B and C and is active over a wide pH range and in at least 1 mM DTT.

Cat. #	Product	Size	Price
2313B	Recombinant RNase Inhibitor	25,000 Units	USD $671.00
Recombinant RNase Inhibitor forms a 1:1 complex with RNase A and inhibits RNase activity. This reaction is reversible, and ribonuclease activity can be recovered by dissociating the complex with urea or sulfhydryl reagent. This reaction irreversibly inactivates the inhibitor. Recombinant RNase Inhibitor can be added directly to reaction mixtures containing RNA. In addition, because it is a protein, it differs from other competitive inhibitors (nucleotides and inorganic phosphates) by being able to be easily removed from the reaction system using phenol extraction. Cat. # 2313B contains 5 of Cat. # 2313A. Please refer to Cat. # 2313A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 2313B: Recombinant RNase Inhibitor
2313A	Recombinant RNase Inhibitor	5,000 Units	USD $165.00
Recombinant RNase Inhibitor forms a 1:1 complex with RNase A and inhibits RNase activity. This reaction is reversible, and ribonuclease activity can be recovered by dissociating the complex with urea or sulfhydryl reagent. This reaction irreversibly inactivates the inhibitor. Recombinant RNase Inhibitor can be added directly to reaction mixtures containing RNA. In addition, because it is a protein, it differs from other competitive inhibitors (nucleotides and inorganic phosphates) by being able to be easily removed from the reaction system using phenol extraction. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 2313A: Recombinant RNase Inhibitor

More Information

Applications

cDNA synthesis (Ribonuclease Inhibitor: 0.5 U/µl reaction)
In vitro translation (Ribonuclease Inhibitor: 1 U/µl reaction)
In vitro transcription using cell-free extract (Ribonuclease Inhibitor: 20 U/µl reaction)
In vitro transcription with SP6 or T7 RNA Polymerase (Ribonuclease Inhibitor: 1 U/µl reaction)
Polysome isolation (Ribonuclease Inhibitor: 1,000 U/ml reaction)

Components

2313A: 5,000 U

2313B: 5 x 2313A (25,000 U)

Product citations

Blackburn, P. Ribonuclease inhibitor from human placenta: rapid purification and assay. J. Biol. Chem. 254, 12484–7 (1979).

Blackburn, P., Wilson, G. & Moore, S. Ribonuclease inhibitor from human placenta. Purification and properties. J. Biol. Chem. 252, 5904–10 (1977).

Burton, L. E. & Fucci, N. P. Ribonuclease inhibitors from the livers of five mammalian species. Int. J. Pept. Protein Res. 19, 372–9 (1982).

Eichler, D. C., Tatar, T. F. & Lasater, L. S. Effect of human placental ribonuclease inhibitor in cell-free ribosomal RNA synthesis. Biochem. Biophys. Res. Commun. 101, 396–403 (1981).

Martynoff, G. de, Pays, E. & Vassart, G. Synthesis of a full-length DNA complementary to thyroglobulin 33 S messenger RNA. Biochem. Biophys. Res. Commun. 93, 645–653 (1980).

Scheele, G. & Blackburn, P. Role of mammalian RNase inhibitor in cell-free protein synthesis. Proc. Natl. Acad. Sci. U. S. A. 76, 4898–902 (1979).

Turner, P. M., Lerea, K. M. & Kull, F. J. The ribonuclease inhibitors from porcine thyroid and liver are slow, tight-binding inhibitors of bovine pancreatic ribonuclease A. Biochem. Biophys. Res. Commun. 114, 1154–60 (1983).

Unit definition

One unit is defined as the amount of Recombinant Ribonuclease Inhibitor required to inhibit the activity of 5 ng of RNase A by 50%. Activity is determined as the ability to inhibit hydrolysis of cytidine 2,3'-cyclic monophosphate (cyclic CMP) by RNAse A.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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