Recombinant Ribonuclease Inhibitor, GPR
Takara Bio's Recombinant Ribonuclease Inhibitor, GPR is a registered general purpose reagent (GPR) appropriate for use in general laboratory applications, including molecular diagnostic development and testing. It is a recombinant protein that acts as a noncompetitive RNase inhibitor of RNases A, B, and C. This RNase inhibitor is active over a wide pH range and in at least 1 mM DTT.
Overview
- Manufactured and labeled in accordance with requirements for general purpose reagents
More Information
Applications
- cDNA synthesis (Ribonuclease Inhibitor: 0.5 U/µl reaction)
- In vitro translation (Ribonuclease Inhibitor: 1 U/µl reaction)
- In vitro transcription using cell-free extract (Ribonuclease Inhibitor: 20 U/µl reaction)
- In vitro transcription with SP6 or T7 RNA Polymerase (Ribonuclease Inhibitor: 1 U/µl reaction)
- Polysome isolation (Ribonuclease Inhibitor: 1,000 U/ml reaction)
Components
2314A: 5,000 U
2314B: 5 x 2313A (25,000 U)
Source
E. coli containing a plasmid that carries the porcine RNase Inhibitor gene
Storage
–20°C
Concentration
40 U/µl
Product citations
Blackburn, P. Ribonuclease inhibitor from human placenta: rapid purification and assay. J. Biol. Chem. 254, 12484–7 (1979).
Blackburn, P., Wilson, G. & Moore, S. Ribonuclease inhibitor from human placenta. Purification and properties. J. Biol. Chem. 252, 5904–10 (1977).
Burton, L. E. & Fucci, N. P. Ribonuclease inhibitors from the livers of five mammalian species. Int. J. Pept. Protein Res. 19, 372–9 (1982).
Eichler, D. C., Tatar, T. F. & Lasater, L. S. Effect of human placental ribonuclease inhibitor in cell-free ribosomal RNA synthesis. Biochem. Biophys. Res. Commun. 101, 396–403 (1981).
de Martynoff, G., Pays, E. & Vassart, G. Synthesis of a full length DNA complementary to thyroglobulin 33 S messenger RNA. Biochem. Biophys. Res. Commun. 93, 645–53 (1980).
Scheele, G. & Blackburn, P. Role of mammalian RNase inhibitor in cell-free protein synthesis. Proc. Natl. Acad. Sci. U. S. A. 76, 4898–902 (1979).
Turner, P. M., Lerea, K. M. & Kull, F. J. The ribonuclease inhibitors from porcine thyroid and liver are slow, tight-binding inhibitors of bovine pancreatic ribonuclease A. Biochem. Biophys. Res. Commun. 114, 1154–60 (1983).
Unit definition
One unit is defined as the amount of Recombinant Ribonuclease Inhibitor, GPR required to inhibit the activity of 5 ng of RNase A by 50%. Activity is determined as the ability to inhibit hydrolysis of cytidine 2,3'-cyclic monophosphate (cyclic CMP) by RNAse A.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
NOTICE TO PURCHASER: General Purpose Reagent. This product is intended For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated. This product is not intended for a specific application or made for any clinical use. The performance characteristics of this product have not been fully established. It is the user's responsibility to validate the performance of the product, and any component thereof, for any particular use. Resale or transfer of this product, any component thereof, or any substance produced through use of this the product, or any component thereof, to any third party is expressly forbidden. To obtain additional rights, please contact oem@takarabio.com.
Your use of this product is also subject to compliance with the licensing requirements listed below and described on the product´s web page at takarabio.com. It is your responsibility to review, understand and adhere to any restrictions imposed by these statements.
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