SMART-Seq Mouse BCR (with UMIs)
SMART-Seq Mouse BCR (with UMIs) enables detection of mouse BCR sequences from all BCR isotypes (IgA/D/E/G/M on heavy chain; IgK/L on light chain). Powered by SMART (Switching Mechanism at 5' end of RNA Template) technology, the kit combines NGS with a 5’-RACE like approach to capture the full-length V(D)J sequences of mouse B-cell receptors.
SMART-Seq Mouse BCR (with UMIs) enables detection of mouse BCR sequences from all BCR isotypes (IgA/D/E/G/M on heavy chain; IgK/L on light chain). Powered by SMART (Switching Mechanism at 5' end of RNA Template) technology, the kit combines NGS with a 5’-RACE like approach to capture the full-length V(D)J sequences of mouse B-cell receptors.
The kit is designed to work with high-quality mouse RNA (RIN>7) spanning a wide range: 10 ng–1 μg of total RNA from mouse spleen, bone marrow, or peripheral blood mononuclear cells (PBMCs). It also works with other sample types such as RNA extracted from mouse blood or lymph nodes.
BCR library preparation can now be streamlined with provided primer pools and enzyme premix, reducing the number of tubes, reagents, and steps. Unique molecular identifiers (UMIs) are also incorporated into the workflow to remove PCR bias and sequencing errors during data analysis, thus guaranteeing accuracy. Moreover, this kit is compatible with the Unique Dual Index Kits (Cat. # 634752–56; sold separately) so up to 384 BCR libraries can be pooled together in a single flow cell to save sequencing cost. Protocol is also automation friendly, so you can easily miniaturize to reduce the cost-per-reaction and increase throughput.
With this kit, you can also enjoy sequencing flexibility and the library products can be sequenced on any Illumina sequencer. The full-length V(D)J area can be sequenced if more data is desired, or you can focus on just the CDR3 region to save on sequencing costs.
Overview
- Complete BCR repertoire profiling: includes all heavy (IgG, IgM, IgA, IgD, IgE) and light (IgK, IgL) BCR chains
- Supports various inputs: 10 ng–1 μg of high-integrity RNA (RIN>7) from mouse spleen, bone marrow, lymph nodes, whole blood, and PBMCs
- Streamlined workflow: primer pools for heavy and light immunoglobulin chains and enzyme premixes provided
- Increased accuracy: UMI-based analysis controls for PCR duplicates and errors, correcting for artifacts in clonotype diversity and abundance assessments
- Flexible: sequence either the full-length V(D)J region or the CDR3 only and multiplex with up to 384 UDIs
- Economical and high-throughput friendly: can be miniaturized to reduce costs or automated
Achieve sensitive and reproducible BCR clonotype detection
SMART-Seq Mouse BCR (with UMIs) detected all BCR isotypes with outstanding sensitivity. Mouse BCR heavy chain (IgA/D/G/E/M) and light chain (IgK/L) libraries were generated from 10, 100, and 1,000 ng of mouse spleen RNA using SMART-Seq Mouse BCR (with UMIs). The sequencing reads were processed using Cogent NGS Immune Profiler Software.
More Information
Applications
- Analyze B-cell clonal organization and diversity in tissues and circulation to understand how they affect B-cell differentiation pathway and functionalities.
- Find BCRs with high affinity to inform better vaccination or therapy against infectious diseases.
- Track changes and migration of antigen-specific B cells post adoptive transfer in mouse disease models for HIV and other diseases.
- Check BCR sequences to study development of autoantibodies against new epitopes over time and how that correlates with severity of autoimmune diseases like lupus.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2025 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
