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Immune profiling

SMART-Seq Mouse BCR (with UMIs)

SMART-Seq Mouse BCR (with UMIs) enables detection of mouse BCR sequences from all BCR isotypes (IgA/D/E/G/M on heavy chain; IgK/L on light chain). Powered by SMART (Switching Mechanism at 5' end of RNA Template) technology, the kit combines NGS with a 5’-RACE like approach to capture the full-length V(D)J sequences of mouse B-cell receptors.

The kit is designed to work with high-quality mouse RNA (RIN>7) spanning a wide range: 10 ng–1 μg of total RNA from mouse spleen, bone marrow, or peripheral blood mononuclear cells (PBMCs). It also works with other sample types such as RNA extracted from mouse blood or lymph nodes.

BCR library preparation can now be streamlined with provided primer pools and enzyme premix, reducing the number of tubes, reagents, and steps. Unique molecular identifiers (UMIs) are also incorporated into the workflow to remove PCR bias and sequencing errors during data analysis, thus guaranteeing accuracy. Moreover, this kit is compatible with the Unique Dual Index Kits (Cat. # 634752–56; sold separately) so up to 384 BCR libraries can be pooled together in a single flow cell to save sequencing cost. Protocol is also automation friendly, so you can easily miniaturize to reduce the cost-per-reaction and increase throughput.

With this kit, you can also enjoy sequencing flexibility and the library products can be sequenced on any Illumina sequencer. The full-length V(D)J area can be sequenced if more data is desired, or you can focus on just the CDR3 region to save on sequencing costs.

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Cat. #

Product

Size

Price

License

Quantity

Details

634352

SMART-Seq® Mouse BCR (with UMIs)

24 Rxns

USD $1347.00

License Statement

ID Number

392

This Product is protected by one or more patents from the family consisting of:US10941397, People's Republic of China Patent: ZL201480057094.4, US10781443, US10954510, DE602014069266.4, EP3058104, FR3058104, UK3058104, JP6602294, SE3058104, CA2923812, and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family. Information on relevant patents and licenses for this product may be found at: https://www.takarabio.com/patents.

455

This product is sold under license from Becton Dickinson and Co., and may be the subject of U.S. Patent Nos.: 8,835,358; 9,290,809; 9,315,857; 9,708,659; 9,845,502; 10,047,394; 10,059,991; 10,202,646; 10,392,661; 10,619,203; 11,970,737; 12,060,607; and its foreign counterparts.

SMART-Seq Mouse BCR (with UMIs) enables users to analyze B-cell receptor (BCR) repertoires from 10 ng–1 µg high-integrity (RIN>7) bulk total RNA samples from spleen, bone marrow, or peripheral blood mononuclear cells (PBMCs) or RNA extracted from mouse whole blood (RNA input ≥100 ng) or lymph nodes. The kit can be used to generate data for both heavy (IgG, IgM, IgA, IgD, and IgE) and light chains (IgK, IgL) of mouse B-cell receptors.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

Required Products

Cat. #	Product	Size	Price
634752	Unique Dual Index Kit (1–96)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634752: Unique Dual Index Kit (1-96)
634753	Unique Dual Index Kit (97–192)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634753: Unique Dual Index Kit (97-192)
634754	Unique Dual Index Kit (193–288)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634754: Unique Dual Index Kit (193-288)
634755	Unique Dual Index Kit (289–384)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634755: Unique Dual Index Kit (289-384)
634756	Unique Dual Index Kit (1–24)	24 Rxns	USD $222.00
The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634756: Unique Dual Index Kit (1-24)
744970.5	NucleoMag® NGS Clean-up and Size Select	5 mL	USD $109.00
NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization. Cat. # 744970.5 consists of a bottle containing 5 ml of bead suspension. Documents Image Data Back Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment. Back Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%). Back Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp. Back Fragment size analysis for NGS libraries prepared using size-selection beads from various providers Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads. Back Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads. Back 744970.5: NucleoMag NGS Clean-up and Size Select
744970.50	NucleoMag® NGS Clean-up and Size Select	50 mL	USD $745.00
NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization. Cat. # 744970.50 consists of a bottle containing 50 ml of bead suspension. Documents Image Data Back Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment. Back Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%). Back Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp. Back Fragment size analysis for NGS libraries prepared using size-selection beads from various providers Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads. Back Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads. Back 744970.50: NucleoMag NGS Clean-up and Size Select
744970.500	NucleoMag® NGS Clean-up and Size Select	500 mL	USD $5155.00
NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization. Cat. # 744970.500 consists of a bottle containing 500 ml of bead suspension. Documents Image Data Back Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment. Back Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%). Back Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp. Back Fragment size analysis for NGS libraries prepared using size-selection beads from various providers Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads. Back Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads. Back 744970.500: NucleoMag NGS Clean-up and Size Select

634353

SMART-Seq® Mouse BCR (with UMIs)

96 Rxns

USD $4795.00

License Statement

ID Number

392

455

Notice to purchaser

Components

Required Products

Cat. #	Product	Size	Price
634752	Unique Dual Index Kit (1–96)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634752: Unique Dual Index Kit (1-96)
634753	Unique Dual Index Kit (97–192)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634753: Unique Dual Index Kit (97-192)
634754	Unique Dual Index Kit (193–288)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634754: Unique Dual Index Kit (193-288)
634755	Unique Dual Index Kit (289–384)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634755: Unique Dual Index Kit (289-384)
634756	Unique Dual Index Kit (1–24)	24 Rxns	USD $222.00
The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634756: Unique Dual Index Kit (1-24)
744970.5	NucleoMag® NGS Clean-up and Size Select	5 mL	USD $109.00
NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization. Cat. # 744970.5 consists of a bottle containing 5 ml of bead suspension. Documents Image Data Back Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment. Back Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%). Back Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp. Back Fragment size analysis for NGS libraries prepared using size-selection beads from various providers Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads. Back Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads. Back 744970.5: NucleoMag NGS Clean-up and Size Select
744970.50	NucleoMag® NGS Clean-up and Size Select	50 mL	USD $745.00
NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization. Cat. # 744970.50 consists of a bottle containing 50 ml of bead suspension. Documents Image Data Back Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment. Back Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%). Back Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp. Back Fragment size analysis for NGS libraries prepared using size-selection beads from various providers Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads. Back Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads. Back 744970.50: NucleoMag NGS Clean-up and Size Select
744970.500	NucleoMag® NGS Clean-up and Size Select	500 mL	USD $5155.00
NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization. Cat. # 744970.500 consists of a bottle containing 500 ml of bead suspension. Documents Image Data Back Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment. Back Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%). Back Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp. Back Fragment size analysis for NGS libraries prepared using size-selection beads from various providers Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads. Back Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads. Back 744970.500: NucleoMag NGS Clean-up and Size Select

634351

SMART-Seq® Mouse BCR (with UMIs)

4 x 96 Rxns

USD $17472.00

License Statement

ID Number

392

Notice to purchaser

Components

Required Products

Cat. #	Product	Size	Price
634752	Unique Dual Index Kit (1–96)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634752: Unique Dual Index Kit (1-96)
634753	Unique Dual Index Kit (97–192)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634753: Unique Dual Index Kit (97-192)
634754	Unique Dual Index Kit (193–288)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634754: Unique Dual Index Kit (193-288)
634755	Unique Dual Index Kit (289–384)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634755: Unique Dual Index Kit (289-384)
634756	Unique Dual Index Kit (1–24)	24 Rxns	USD $222.00
The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634756: Unique Dual Index Kit (1-24)
744970.5	NucleoMag® NGS Clean-up and Size Select	5 mL	USD $109.00
NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization. Cat. # 744970.5 consists of a bottle containing 5 ml of bead suspension. Documents Image Data Back Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment. Back Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%). Back Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp. Back Fragment size analysis for NGS libraries prepared using size-selection beads from various providers Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads. Back Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads. Back 744970.5: NucleoMag NGS Clean-up and Size Select
744970.50	NucleoMag® NGS Clean-up and Size Select	50 mL	USD $745.00
NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization. Cat. # 744970.50 consists of a bottle containing 50 ml of bead suspension. Documents Image Data Back Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment. Back Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%). Back Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp. Back Fragment size analysis for NGS libraries prepared using size-selection beads from various providers Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads. Back Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads. Back 744970.50: NucleoMag NGS Clean-up and Size Select
744970.500	NucleoMag® NGS Clean-up and Size Select	500 mL	USD $5155.00
NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization. Cat. # 744970.500 consists of a bottle containing 500 ml of bead suspension. Documents Image Data Back Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment. Back Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%). Back Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp. Back Fragment size analysis for NGS libraries prepared using size-selection beads from various providers Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads. Back Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads. Back 744970.500: NucleoMag NGS Clean-up and Size Select

Overview

Complete BCR repertoire profiling: includes all heavy (IgG, IgM, IgA, IgD, IgE) and light (IgK, IgL) BCR chains
Supports various inputs: 10 ng–1 μg of high-integrity RNA (RIN>7) from mouse spleen, bone marrow, lymph nodes, whole blood, and PBMCs
Streamlined workflow: primer pools for heavy and light immunoglobulin chains and enzyme premixes provided
Increased accuracy: UMI-based analysis controls for PCR duplicates and errors, correcting for artifacts in clonotype diversity and abundance assessments
Flexible: sequence either the full-length V(D)J region or the CDR3 only and multiplex with up to 384 UDIs
Economical and high-throughput friendly: can be miniaturized to reduce costs or automated

Achieve sensitive and reproducible BCR clonotype detection

images/400-NGS/CC-TechFigs/SMART-Seq-Mouse-BCR-with-UMIs-BCR-isotypes-9512A-iT.gif

More Information

Applications

Analyze B-cell clonal organization and diversity in tissues and circulation to understand how they affect B-cell differentiation pathway and functionalities.
Find BCRs with high affinity to inform better vaccination or therapy against infectious diseases.
Track changes and migration of antigen-specific B cells post adoptive transfer in mouse disease models for HIV and other diseases.
Check BCR sequences to study development of autoantibodies against new epitopes over time and how that correlates with severity of autoimmune diseases like lupus.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Takara Bio USA, Inc.
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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2024 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

SMART-Seq Mouse BCR (with UMIs)

Notice to purchaser

Schematic of technology and workflow for SMART-Seq Mouse BCR (with UMIs)

mBCRv2 detected all BCR isotypes with outstanding sensitivity

634352: SMART-Seq Mouse BCR (with UMIs)

634752: Unique Dual Index Kit (1-96)

634753: Unique Dual Index Kit (97-192)

634754: Unique Dual Index Kit (193-288)

634755: Unique Dual Index Kit (289-384)

634756: Unique Dual Index Kit (1-24)

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

744970.5: NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

744970.50: NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

744970.500: NucleoMag NGS Clean-up and Size Select

Notice to purchaser

Schematic of technology and workflow for SMART-Seq Mouse BCR (with UMIs)

mBCRv2 detected all BCR isotypes with outstanding sensitivity

634353: SMART-Seq Mouse BCR (with UMIs)

634752: Unique Dual Index Kit (1-96)

634753: Unique Dual Index Kit (97-192)

634754: Unique Dual Index Kit (193-288)

634755: Unique Dual Index Kit (289-384)

634756: Unique Dual Index Kit (1-24)

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

744970.5: NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

744970.50: NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

744970.500: NucleoMag NGS Clean-up and Size Select

Notice to purchaser

Schematic of technology and workflow for SMART-Seq Mouse BCR (with UMIs)

mBCRv2 detected all BCR isotypes with outstanding sensitivity

634351: SMART-Seq Mouse BCR (with UMIs)

634752: Unique Dual Index Kit (1-96)

634753: Unique Dual Index Kit (97-192)

634754: Unique Dual Index Kit (193-288)

634755: Unique Dual Index Kit (289-384)

634756: Unique Dual Index Kit (1-24)

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

744970.5: NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

744970.50: NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers