TaKaRa TaqDNA Polymerase is a recombinant version Taq polymerase derived from the Thermus aquaticusYT-1 strain, and is suitable for routine PCR applications.
For individual reaction setup and optimization, use individual components of enzyme, dNTP mixture, and 10X reaction buffer (with or without Mg2+). A convenient 2X PCR master mix of Takara Taq enzyme, buffer, and dNTP mixture is available as Premix Taq DNA Polymerase (TaKaRa Taq Version 2.0) (Cat. # R004A).
Premix Taq enables easy PCR setup and minimizes pipetting steps
More Information
Routine PCR
DNA sequencing
Source
Recombinant E. coli
PCR products
The resulting PCR product can be cloned directly into a T-vector (TA cloning) due to the addition of an adenosine (A) to the 3' end. It is also possible to clone the PCR product into blunt-end vectors after blunting and phosphorylation of the ends.
Bulk, custom, and OEM information
If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.