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Tet-inducible expression systems

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Tet-On 3G cell lines for tetracycline-inducible expression

Ready-made cell lines expressiong the Tet-On 3G tetracycline transactivator

Tet-On 3G cell lines greatly simplify the task of setting up a tetracycline-inducible gene expression system that is activated by adding doxycycline (Dox) to the culture medium. These rigorously tested clonal cell lines stably express our premium Tet-On 3G transactivator. They allow you to induce expression of your gene of interest by adding Dox to the culture of transfected cells, and can save you up to two months of time in developing a complete Tet-controlled system.

Tet-On 3G cell lines greatly simplify the task of setting up a tetracycline-inducible gene expression system that is activated by adding doxycycline (Dox) to the culture medium. These rigorously tested clonal cell lines stably express our premium Tet-On 3G transactivator. They allow you to induce expression of your gene of interest by adding Dox to the culture of transfected cells, and can save you up to two months of time in developing a complete Tet-controlled system.

Easily create an inducible system by placing your gene under the control of PTRE3G, transfecting it into one of these G418-resistant cell lines, and selecting for double-stable clones.

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Cat. # Product Size Price License Quantity Details
631195 CHO Tet-On® 3G Cell Line Each USD $1237.00

CHO Tet-On 3G is a an epithelial-like cell line derived from Chinese hamster ovary (CHO-K1 clone) that expresses the tetracycline (Tet)-regulated transactivator Tet-On 3G. Inducible expression of any gene can be achieved by transfecting or transducing this cell line with a vector containing your gene of interest under the control of a tetracycline-responsive promoter. Expression is induced by the addition of doxycycline (Dox) to the culture medium.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components

Required Products

Cat. # Product Size Price License Quantity Details
631101 Tet System Approved FBS, US-Sourced 500 mL USD $902.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

Documents Components Image Data
631106 Tet System Approved FBS 500 mL USD $883.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Documents Components Image Data
631183 HeLa Tet-On® 3G Cell Line Each USD $1237.00

HeLa Tet-On 3G is a human cervical epithelioid carcinoma-derived cell line that expresses the tetracycline (Tet)-regulated transactivator Tet-On 3G. Inducible expression of any gene can be achieved by transfecting or transducing this cell line with a vector containing your gene of interest under the control of a tetracycline-responsive promoter. Expression is induced by the addition of doxycycline (Dox) to the culture medium. Tet-inducible cell lines are shipped with Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Required Products

Cat. # Product Size Price License Quantity Details
631101 Tet System Approved FBS, US-Sourced 500 mL USD $902.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

Documents Components Image Data
631106 Tet System Approved FBS 500 mL USD $883.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Documents Components Image Data
631182 HEK 293 Tet-On® 3G Cell Line Each USD $1237.00

HEK 293 Tet-On 3G is a transformed human embryonic kidney-derived cell line that expresses the tetracycline (Tet)-regulated transactivator Tet-On 3G. Inducible expression of any gene can be achieved by transfecting or transducing this cell line with a vector containing your gene of interest under the control of a tetracycline-responsive promoter. Expression is induced by the addition of doxycycline (Dox) to the culture medium. Tet-inducible cell lines are shipped with Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Required Products

Cat. # Product Size Price License Quantity Details
631101 Tet System Approved FBS, US-Sourced 500 mL USD $902.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

Documents Components Image Data
631106 Tet System Approved FBS 500 mL USD $883.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Documents Components Image Data

FBS for Tet system Tet-approved FBS
Overviews Tet-One overview
Use TetR monoclonal antibody to detect tetracycline transactivators on Western blots TetR antibody

Overview

  • Cell lines with high inducibility
  • Rapidly create an inducible expression system for your gene of interest.
  • Premade Tet-responsive cell lines reduce development time/effort by half
  • Save up to two months of time in developing a complete Tet-controlled system
  • Use in combination with any Tet-On 3G inducible expression system

More Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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