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Lentivirus

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Selection guides Lentiviral vector options

Lentiviral vectors with fluorescent proteins

Lentivirus expressing fluorescent proteins

These lentiviral vectors allow you to deliver and stably express your gene of interest as a fluorescent fusion protein in virtually any cell type, including dividing and nondividing cells, stem cells, terminally differentiated cells, and neuronal cells. Three bicistronic lentiviral vectors (pLVX-IRES-mCherry, pLVX-IRES-tdTomato, and pLVX-IRES-ZsGreen1) are also available to allow your gene of interest and a bright fluorescent protein to be coexpressed from a single mRNA transcript.

These lentiviral vectors allow you to deliver and stably express your gene of interest as a fluorescent fusion protein in virtually any cell type, including dividing and nondividing cells, stem cells, terminally differentiated cells, and neuronal cells. Three bicistronic lentiviral vectors (pLVX-IRES-mCherry, pLVX-IRES-tdTomato, and pLVX-IRES-ZsGreen1) are also available to allow your gene of interest and a bright fluorescent protein to be coexpressed from a single mRNA transcript. Takara Bio’s Living Colors DsRed-Monomer, AcGFP1, and mCherry proteins are truly monomeric fluorescent proteins that are ideal for use as fusion tags. ZsGreen1 and tdTomato are supremely bright fluorescent proteins that are excellent reporters for gene expression, or for measuring transduction/transfection efficiency. mCherry is a very popular fluorescent protein derived by directed mutagenesis of mRFP1, a monomeric mutant of DsRed. DsRed-Express2 is a rapidly maturing variant of Discosoma sp. red fluorescent protein (DsRed). It is highly soluble and was designed to be better suited for cell and stem cell applications.

All Living Colors proteins are detectable without added cofactors or substrates, making them exceptional, noninvasive tools for live-cell studies.

DsRed-Monomer & AcGFP1

  • Truly monomeric proteins (see DsRed-Monomer for more information)
  • Ideal for tagging proteins for trafficking and localization studies
  • Distinct spectra make them excellent choices for double labeling experiments

ZsGreen1 & tdTomato

  • The brightest green and red fluorescent proteins—(2.5X and 3.5X brighter than EGFP, respectively)
  • IRES bicistronic expression for monitoring transduction efficiency
  • Separate expression of your cDNA and a fluorescent protein from a single transcript

mCherry

  • A bright monomeric protein
  • Matures very rapidly—its t1/2 for maturation at 37°C is approximately 15 minutes

DsRed-Express2

  • Rapidly maturing red fluorescent protein with enhanced solubility and reduced green emission
  • Well-suited for cell and stem cell applications
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Cat. # Product Size Price License Quantity Details
632587 pLVX-PAmCherry-C1 Vector 10 ug USD $693.00

pLVX-PAmCherry-C1 Vector is an HIV-1-based, lentiviral expression vector. Lentiviral particles derived from the vector allow you to infect cells and express your gene of interest fused to PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry. PAmCherry is non-fluorescent until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm. Genes cloned into the MCS will be expressed as fusions to the C-terminus of PAmCherry if they are in the same reading frame as PAmCherry and there are no intervening stop codons.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632586 pLVX-PAmCherry-N1 Vector 10 ug USD $693.00

pLVX-PAmCherry-N1 Vector is an HIV-1-based, lentiviral expression vector. Lentiviral particles derived from the vector allow you to infect cells and express your gene of interest fused to PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry. PAmCherry is non-fluorescence until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm. Genes cloned into the MCS will be expressed as fusions to the N-terminus of PAmCherry if they are in the same reading frame as PAmCherry and there are no intervening stop codons.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632566 pLVX-ZsGreen1-C1 Vector 10 ug USD $676.00

This lentiviral expression vector encodes the ZsGreen1 fluorescent protein tag. The coding sequence for this very bright green fluorescent protein (2.5x brighter than EGFP) was modified to include human codon usage preferences, which allow improved expression in mammalian cells. Inserting a cDNA in the MCS downstream of the ZsGreen1 coding sequence joins your protein of interest to the C-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells. To package the vector into high-titer, replication-incompetent lentivirus, we recommend using Lenti-X Packaging Single Shots and the Lenti-X 293T Cell Line. The resulting lentivirus can then be used to transduce virtually any mammalian cell type.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632565 pLVX-ZsGreen1-N1 Vector 10 ug USD $676.00

This lentiviral expression vector encodes the ZsGreen1 fluorescent protein tag. The coding sequence for this very bright green fluorescent protein (2.5x brighter than EGFP) was modified to include human codon usage preferences, which allow improved expression in mammalian cells. Inserting a cDNA in the MCS upstream of the ZsGreen1 coding sequence joins your protein of interest to the N-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells.

To package the vector into high-titer, replication-incompetent lentivirus, we recommend using Lenti-X Packaging Single Shots and the Lenti-X 293T Cell Line. The resulting lentivirus can then be used to transduce virtually any mammalian cell type.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632564 pLVX-tdTomato-C1 Vector 10 ug USD $676.00

This lentiviral expression vector encodes the tdTomato fluorescent protein tag. This very bright red fluorescent protein is a genetic fusion of two copies of dTomato, which was specifically designed for low aggregation. It adopts an intramolecular tandem dimer structure that contributes to its exceptional brightness, yet it behaves like a monomer. Inserting a cDNA in the MCS downstream of the tdTomato coding sequence joins your protein of interest to the C-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells.

To package the vector into high-titer, replication-incompetent lentivirus, we recommend using Lenti-X Packaging Single Shots and the Lenti-X 293T Cell Line. The resulting lentivirus can then be used to transduce virtually any mammalian cell type.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632563 pLVX-tdTomato-N1 Vector 10 ug USD $676.00

This lentiviral expression vector encodes the tdTomato fluorescent protein tag. This very bright red fluorescent protein is a genetic fusion of two copies of dTomato, which was specifically designed for low aggregation. It adopts an intramolecular tandem dimer structure that contributes to its exceptional brightness, yet it behaves like a monomer. Inserting a cDNA in the MCS upstream of the tdTomato coding sequence joins your protein of interest to the N-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells.

To package the vector into high-titer, replication-incompetent lentivirus, we recommend using Lenti-X Packaging Single Shots and the Lenti-X 293T Cell Line. The resulting lentivirus can then be used to transduce virtually any mammalian cell type.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632562 pLVX-mCherry-N1 Vector 10 ug USD $676.00

This lentiviral expression vector encodes an mCherry fluorescent protein tag. This bright red fluorescent protein was derived by site-directed mutagenesis of mRFP1, a monomeric mutant of DsRed. Inserting a cDNA in the MCS upstream of the mCherry coding sequence joins your protein of interest to the N-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells. To package the vector into high-titer, replication-incompetent lentivirus, we recommend using Lenti-X Packaging Single Shots and the Lenti-X 293T Cell Line. The resulting lentivirus can then be used to transduce virtually any mammalian cell type.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632561 pLVX-mCherry-C1 Vector 10 ug USD $676.00

This lentiviral expression vector encodes an mCherry fluorescent protein tag. This bright red fluorescent protein was derived by site-directed mutagenesis of mRFP1, a monomeric mutant of DsRed. Inserting a cDNA in the MCS downstream of the mCherry coding sequence joins your protein of interest to the C-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells. To package the vector into high-titer, replication-incompetent lentivirus, we recommend using Lenti-X Packaging Single Shots and the Lenti-X 293T Cell Line. The resulting lentivirus can then be used to transduce virtually any mammalian cell type.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632560 pLVX-DsRed-Express2-N1 Vector 10 ug USD $676.00

This lentiviral expression vector encodes a DsRed-Express2 fluorescent protein tag. The protein is a rapidly maturing tetrameric fluorescent protein from Discosoma sp. that has minimal cytotoxicity, high solubility, and exhibits strong and stable expression mammalian cells. Inserting a cDNA in the MCS upstream of the DsRed-Express2 coding sequence joins your protein of interest to the N-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells. To package the vector into high-titer, replication-incompetent lentivirus, we recommend using the Lenti-X Packaging Single Shots and the Lenti-X 293T Cell Line. The resulting lentivirus can then be used to transduce virtually any mammalian cell type.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632559 pLVX-DsRed-Express2-C1 Vector 10 ug USD $676.00

This lentiviral expression vector encodes a DsRed-Express2 fluorescent protein tag. The protein is a rapidly maturing tetrameric fluorescent protein from Discosoma sp. that has minimal cytotoxicity, high solubility, and exhibits strong and stable expression in mammalian cells. Inserting a cDNA in the MCS downstream of the DsRed-Express2 coding sequence joins your protein of interest to the C-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells. To package the vector into high-titer, replication-incompetent lentivirus, we recommend using the Lenti-X Packaging Single Shots (VSV-G) and the Lenti-X 293T Cell Line. The resulting lentivirus can then be used to transduce virtually any mammalian cell type.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632558 pLVX-AmCyan1-N1 Vector 10 ug USD $676.00

This lentiviral expression vector encodes an AmCyan1 fluorescent protein tag. The protein is a human codon-optimized variant of the Anemonia majano cyan fluorescent protein engineered for increased brightness and higher expression in mammalian cells. Inserting a cDNA in the MCS upstream of the AmCyan1 coding sequence joins your protein of interest to the N-terminus of AmCyan1, and allows it to be tracked and studied in transduced cells. To package the vector into high-titer, replication-incompetent lentivirus, we recommend using Lenti-X Packaging Single Shots (Cat. Nos. 631275-631278) and the Lenti-X 293T Cell Line (Cat. No. 632180). The resulting lentivirus can then be used to transduce virtually any mammalian cell type.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632557 pLVX-AmCyan1-C1 Vector 10 ug USD $676.00

This lentiviral expression vector encodes an AmCyan1 fluorescent protein tag. The protein is a human codon-optimized variant of the Anemonia majano cyan fluorescent protein engineered for increased brightness and higher expression in mammalian cells. Inserting a cDNA in the MCS downstream of the AmCyan1 coding sequence joins your protein of interest to the C-terminus of AmCyan1, and allows it to be tracked and studied in transduced cells. To package the vector into high-titer, replication-incompetent lentivirus, we recommend using Lenti-X Packaging Single Shots (Cat. Nos. 631275–631278) and the Lenti-X 293T Cell Line (Cat. No. 632180). The resulting lentivirus can then be used to transduce virtually any mammalian cell type.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632187 pLVX-IRES-ZsGreen1 Vector 10 ug USD $676.00

The pLVX-IRES-ZsGreen1 Vector is a bicistronic lentiviral expression vector that can be used to generate high-titer lentivirus for transducing dividing or non-dividing mammalian cells. The vector contains an internal ribosomal entry site (IRES) which allows a gene-of-interest and the ZsGreen1 fluorescent protein to be simultaneously coexpressed from a single mRNA transcript. When used with Lenti-X Packaging Single Shots (e.g. Cat. No. 631275) and our Lenti-X 293T Cell Line (Cat. No. 632180), the vector generates high titers of replication-incompetent, VSV-G pseudotyped lentivirus.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Required Products

Cat. # Product Size Price License Quantity Details
631275 Lenti-X™ Packaging Single Shots (VSV-G) 16 Rxns USD $1033.00

Lenti-X Packaging Single Shots (VSV-G) provide an extremely simple and consistent one-step method for producing high-titer lentivirus. No additional transfection reagent is needed because Lenti-X Packaging Single Shots (VSV-G) consist of pre-aliquoted, lyophilized, single tubes of Xfect Transfection Reagent premixed with an optimized formulation of VSV-G pseudotyped Lenti-X lentiviral packaging plasmids. High-titer virus is produced by simply reconstituting this mixture with your lentiviral vector of choice in sterile water and adding it to 293T cells, e.g., Lenti-X 293T Cells (Cat. # 632180), in a 10 cm dish.

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632180 Lenti-X™ 293T Cell Line 1 mL USD $406.00

The Lenti-X 293T Cell Line is a subclone of the transformed human embryonic kidney cell line, HEK 293, which is highly transfectable and supports high levels of viral protein expression. When transfected with Lenti-X Packaging Single Shots and a lentiviral vector, these cells are capable of producing lentiviral titers as high as >108 ifu/ml, as determined by flow cytometry. The cell line also constitutively expresses the simian virus 40 (SV40) large T antigen.

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632155 pLVX-AcGFP1-C1 Vector 10 ug USD $676.00

The pLVX-AcGFP1-C1 vector is an HIV-1 based lentiviral expression vector that can produce recombinant lentivirus that efficiently transduces both dividing and non-dividing mammalian cells. This vector expresses a variant of the green fluorescent protein (GFP) from Aequorea coerulescens. pLVX-AcGFP1-C1 allows cloning of a cDNA into the multiple cloning site (MCS) downstream of the coding sequence for AcGFP1. The cDNA is subsequently expressed as a fusion to the C-terminus of the AcGFP1 protein. Thus,
this vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable transductants. The unmodified vector will express AcGFP1 protein and may be used to produce marker virus to optimize infection protocols.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632154 pLVX-AcGFP1-N1 Vector 10 ug USD $676.00

The pLVX-AcGFP1-N1 vector is an HIV-1-based lentiviral expression vector that can produce recombinant lentivirus that efficiently transduces both dividing and non-dividing mammalian cells. This vector expresses a variant of the green fluorescent protein (GFP) from Aequorea coerulescens. pLVX-AcGFP1-N1 allows cloning of a cDNA into the multiple cloning site (MCS) upstream of the coding sequence for AcGFP1. The cDNA is subsequently expressed as a fusion to the N-terminus of the AcGFP1 protein. Thus, this vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable transductants. The unmodified vector will express AcGFP1 protein and may be used to produce marker virus to optimize infection protocols.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632153 pLVX-DsRed-Monomer-C1 Vector 10 ug USD $676.00

The pLVX-DsRed-Monomer-C1 vector is an HIV-1-based lentiviral expression vector that can produce lentivirus that efficiently transduces both dividing and non-dividing mammalian cells. This vector expresses a monomeric mutant of the Discosoma sp. red fluorescent protein (DsRed). pLVX-DsRed-Monomer-C1 allows cloning of a cDNA into the multiple cloning site (MCS) downstream of the DsRed-Monomer coding sequence. The cDNA is subsequently expressed as a fusion to the C-terminus of the DsRed-Monomer protein. Thus, this vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable transductants. The unmodified vector will express DsRed-Monomer protein and may be used to produce marker virus to optimize infection protocols.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632152 pLVX-DsRed-Monomer-N1 Vector 10 ug USD $676.00

The pLVX-DsRed-Monomer-N1 vector is an HIV-1-based lentiviral expression vector that can produce recombinant lentivirus that efficiently transduces both dividing and non-dividing mammalian cells. This vector expresses a monomeric mutant of the Discosoma sp. red fluorescent protein (DsRed). pLVX-DsRed-Monomer-N1 allows cloning of a cDNA into the multiple cloning site (MCS) upstream of the DsRed-Monomer coding sequence. The cDNA is subsequently expressed as a fusion to the N-terminus of the DsRed-Monomer protein. Thus, this vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable transductants. The unmodified vector will express DsRed-Monomer protein and may be used to produce marker virus to optimize infection protocols.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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631238 pLVX-IRES-tdTomato Vector 20 ug USD $676.00

The pLVX-IRES-tdTomato Vector is a bicistronic lentiviral expression vector that can be used to generate high-titer lentivirus for transducing dividing or nondividing mammalian cells. The vector contains an internal ribosomal entry site (IRES) which allows a gene-of-interest and the tdTomato fluorescent protein to be simultaneously coexpressed from a single mRNA transcript. When used with the Lenti-X Packaging Single Shots and the Lenti-X 293T Cell Line (Cat. No. 632180), the vector generates high titers of replication-incompetent, VSV-G-pseudotyped lentivirus.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Required Products

Cat. # Product Size Price License Quantity Details
631275 Lenti-X™ Packaging Single Shots (VSV-G) 16 Rxns USD $1033.00

Lenti-X Packaging Single Shots (VSV-G) provide an extremely simple and consistent one-step method for producing high-titer lentivirus. No additional transfection reagent is needed because Lenti-X Packaging Single Shots (VSV-G) consist of pre-aliquoted, lyophilized, single tubes of Xfect Transfection Reagent premixed with an optimized formulation of VSV-G pseudotyped Lenti-X lentiviral packaging plasmids. High-titer virus is produced by simply reconstituting this mixture with your lentiviral vector of choice in sterile water and adding it to 293T cells, e.g., Lenti-X 293T Cells (Cat. # 632180), in a 10 cm dish.

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632180 Lenti-X™ 293T Cell Line 1 mL USD $406.00

The Lenti-X 293T Cell Line is a subclone of the transformed human embryonic kidney cell line, HEK 293, which is highly transfectable and supports high levels of viral protein expression. When transfected with Lenti-X Packaging Single Shots and a lentiviral vector, these cells are capable of producing lentiviral titers as high as >108 ifu/ml, as determined by flow cytometry. The cell line also constitutively expresses the simian virus 40 (SV40) large T antigen.

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631237 pLVX-IRES-mCherry Vector 20 ug USD $676.00

The pLVX-IRES-mCherry Vector is a bicistronic lentiviral expression vector that can be used to generate high-titer lentivirus for transducing dividing or nondividing mammalian cells. The vector contains an internal ribosomal entry site (IRES) which allows a gene-of-interest and the mCherry fluorescent protein to be simultaneously coexpressed from a single mRNA transcript. When used with Lenti-X Packaging Single Shots and the Lenti-X 293T Cell Line (Cat. No. 632180), the vector generates high titers of replication-incompetent, VSV-G-pseudotyped lentivirus.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Required Products

Cat. # Product Size Price License Quantity Details
631275 Lenti-X™ Packaging Single Shots (VSV-G) 16 Rxns USD $1033.00

Lenti-X Packaging Single Shots (VSV-G) provide an extremely simple and consistent one-step method for producing high-titer lentivirus. No additional transfection reagent is needed because Lenti-X Packaging Single Shots (VSV-G) consist of pre-aliquoted, lyophilized, single tubes of Xfect Transfection Reagent premixed with an optimized formulation of VSV-G pseudotyped Lenti-X lentiviral packaging plasmids. High-titer virus is produced by simply reconstituting this mixture with your lentiviral vector of choice in sterile water and adding it to 293T cells, e.g., Lenti-X 293T Cells (Cat. # 632180), in a 10 cm dish.

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631232 Lenti-X™ Concentrator 500 mL USD $938.00

The Lenti-X Concentrator is a complete reagent for the concentration of infectious lentiviral vector particles. This reagent provides a scalable alternative to ultracentrifugation for viral particle concentration. Vector supernatants can be concentrated 10–100 fold, depending upon the volumes used.

Documents Components Image Data

Overviews Premade lentiviral particles
Selection guides Fluorescent protein quick guide
Overviews Lenti-X Concentrator overview

Overview

More Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Lentiviral-mediated fluorescent protein transduction

Lentiviral fluorescent proteins

We offer a wide array of premade lentivirus particles for whole-cell labeling or for labeling subcellular compartments, such as mitochondria, golgi, membranes, etc.

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Tips for a successful transduction

What are the most critical aspects that affect a lentiviral transduction experiment? Learn about upstream and downstream factors affecting lentiviral titer and transduction efficiency.

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