Lentiviral vector systems for constitutive gene expression

The Lenti-X Bicistronic Expression Systems enable you to produce high-titer recombinant lentivirus for the purpose of transferring genes into dividing or non-dividing mammalian cells. The pLVX-IRES-Puro Vector contains an internal ribosome entry site (IRES) followed by a drug resistance marker, so that your gene of interest and puromycin resistance are simultaneously coexpressed from a single mRNA transcript. The Lenti-X packaging system is a proprietary combination of plasmids that collectively expresses all the viral proteins necessary for assembling the vector into infectious virions. Lenti-X Packaging Single Shots, which contain lyophilized Lenti-X packaging plasmids and transfection reagent, are used for efficient introduction of the packaging plasmids and the pLVX-IRES-Puro Vector into Lenti-X 293T Cells (Cat. No. 632180; not included) to produce lentivirus. These optimized reagents allow the transfected Lenti-X 293T cultures to produce high titers of VSV-G pseudotyped lentivirus that is both safe and replication-incompetent, and able to transduce virtually any cell type. The Lenti-X GoStix can be used to determine whether virus production is within a usable range or for selecting the best time to harvest your virus. The system provides enough reagents for 16 packaging reactions.

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Lentiviral vector design

Lentiviral vector design. Clontech's lentiviral vectors contain sequence elements that facilitate lentiviral packaging and boost transgene expression. The Lenti-X Expression System (pLVX-Puro Vector) is used for constitutive expression of your transgene. The Lenti-X Bicistronic Expression Systems allow your transgene and a selectable marker to be coexpressed from a single mRNA transcript.

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4th generation lentiviral packaging system

Fourth-generation lentiviral packaging system. A lentiviral vector and the Lenti-X HTX Packaging Mix are cotransfected into 293T cells. High titer lentiviral supernatants are ready for use 48 hr after transfection.

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High-titer lentivirus production

High-titer lentivirus production. Lenti-X 293T cells were transduced with the indicated volumes (µl) of lentiviral packaging supernatant generated with the Lenti-X Expression System and then selected with puromycin for 9 days to allow the formation of the resistant colonies, which were then stained with crystal violet.

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632183: Lenti-X Bicistronic Expression System (Puro)

The Lenti-X Bicistronic Expression Systems enable you to produce high-titer recombinant lentivirus for the purpose of transferring genes into dividing or non-dividing mammalian cells. The pLVX-IRES-Neo Vector contains an internal ribosome entry site (IRES) followed by a drug resistance marker, so that your gene of interest and hygromycin resistance are simultaneously coexpressed from a single mRNA transcript. The Lenti-X packaging system is a proprietary combination of plasmids that collectively expresses all the viral proteins necessary for assembling the vector into infectious virions. Lenti-X Packaging Single Shots, which contain lyophilized packaging plasmids and transfection reagent, are used for efficient introduction of the Lenti-X packaging mix and the pLVX-IRES-Hyg Vector into Lenti-X 293T Cells (Cat. No. 632180; not included) to produce lentivirus. Tet Approved FBS is supplied to ensure the maximal activity of tetracycline-responsive promoters that are used to express high levels of specific packaging mix proteins. The combined activities of these optimized reagents allows the transfected Lenti-X 293T cultures to produce high titers of VSV-G pseudotyped lentivirus that is both safe and replication-incompetent, and able to transduce virtually any cell type. The Lenti-X GoStix can be used to determine whether virus production is within a usable range or for selecting the best time to harvest your virus. The system provides enough reagents for 16 packaging reactions.

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Lentiviral vector design

Lentiviral vector design. Clontech's lentiviral vectors contain sequence elements that facilitate lentiviral packaging and boost transgene expression. The Lenti-X Expression System (pLVX-Puro Vector) is used for constitutive expression of your transgene. The Lenti-X Bicistronic Expression Systems allow your transgene and a selectable marker to be coexpressed from a single mRNA transcript.

Back

High-titer lentivirus production

High-titer lentivirus production. Lenti-X 293T cells were transduced with the indicated volumes (µl) of lentiviral packaging supernatant generated with the Lenti-X Expression System and then selected with puromycin for 9 days to allow the formation of the resistant colonies, which were then stained with crystal violet.

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632182: Lenti-X Bicistronic Expression System (Hyg)

The Lenti-X Bicistronic Expression Systems enable you to produce high-titer recombinant lentivirus for the purpose of transferring genes into dividing or non-dividing mammalian cells. The pLVX-IRES-Neo Vector contains an internal ribosome entry site (IRES) followed by a drug resistance marker, so that your gene of interest and G418 resistance are simultaneously coexpressed from a single mRNA transcript. The Lenti-X packaging system is a proprietary combination of plasmids that collectively expresses all the viral proteins necessary for assembling the vector into infectious virions. Lenti-X Packaging Single Shots, which contain lyophilized packaging plasmids and transfection reagent, are used for efficient introduction of the Lenti-X packaging mix and the pLVX-IRES-Neo Vector into Lenti-X 293T Cells (Cat. No. 632180; not included) to produce lentivirus. Tet Approved FBS is supplied to ensure the maximal activity of tetracycline-responsive promoters that are used to express high levels of specific packaging mix proteins. The combined activities of these optimized reagents allows the transfected Lenti-X 293T cultures to produce high titers of VSV-G-pseudotyped lentivirus that is both safe and replication-incompetent, and able to transduce virtually any cell type. The Lenti-X GoStix can be used to determine whether virus production is within a usable range or for selecting the best time to harvest your virus. The system provides enough reagents for 16 packaging reactions.

Back

Lentiviral vector design

Lentiviral vector design. Clontech's lentiviral vectors contain sequence elements that facilitate lentiviral packaging and boost transgene expression. The Lenti-X Expression System (pLVX-Puro Vector) is used for constitutive expression of your transgene. The Lenti-X Bicistronic Expression Systems allow your transgene and a selectable marker to be coexpressed from a single mRNA transcript.

Back

High-titer lentivirus production

High-titer lentivirus production. Lenti-X 293T cells were transduced with the indicated volumes (µl) of lentiviral packaging supernatant generated with the Lenti-X Expression System and then selected with puromycin for 9 days to allow the formation of the resistant colonies, which were then stained with crystal violet.

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632181: Lenti-X Bicistronic Expression System (Neo)

The Lenti-X Expression System enables you to produce exceptionally high titers of recombinant lentivirus for expression of any cDNA in any cell type susceptible to lentivirus transduction. The Lenti-X packaging system is a proprietary combination of plasmids that collectively expresses all the viral proteins necessary for high-efficiency packaging of lentiviral vector transcripts into infectious lentivirus particles. Lenti-X Packaging Single Shots, which contain lyophilized Lenti-X packaging plasmids and transfection reagent, are used for efficient introduction of the packaging mix and the pLVX-Puro Vector into 293T cells with very high efficiency. The concerted effects of these components allow 293T cells to produce high-titer replication incompetent lentiviral supernatants for a safe transduction of virtually any cell type. The Lenti-X GoStix can be used to determine whether virus production is within a usable range or for selecting the best time to harvest your virus. The system provides enough reagents for 16 packaging reactions.

Back

Lentiviral vector design

Lentiviral vector design. Clontech's lentiviral vectors contain sequence elements that facilitate lentiviral packaging and boost transgene expression. The Lenti-X Expression System (pLVX-Puro Vector) is used for constitutive expression of your transgene. The Lenti-X Bicistronic Expression Systems allow your transgene and a selectable marker to be coexpressed from a single mRNA transcript.

Back

4th generation lentiviral packaging system

Fourth-generation lentiviral packaging system. A lentiviral vector and the Lenti-X HTX Packaging Mix are cotransfected into 293T cells. High titer lentiviral supernatants are ready for use 48 hr after transfection.

Back

High-titer lentivirus production

High-titer lentivirus production. Lenti-X 293T cells were transduced with the indicated volumes (µl) of lentiviral packaging supernatant generated with the Lenti-X Expression System and then selected with puromycin for 9 days to allow the formation of the resistant colonies, which were then stained with crystal violet.

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632164: Lenti-X Expression System

The Lenti-X Expression System (EF1α Version) enables you to produce exceptionally high titers of recombinant lentivirus for expression of any cDNA in any cell type susceptible to lentiviral transduction. The System contains an HIV-1-based, lentiviral expression vector, pLVX-EF1α-IRES-Puro, designed to constitutively express a protein of interest and puromycin resistance (Puro^r). Constitutive expression of these proteins is driven by the human elongation factor 1 alpha (EF1α) promoter, which allows protein expression in mammalian cells in which the CMV promoter is silenced. The System also includes Lenti-X Packaging Single Shots (VSV-G) and Lenti-X GoStix.

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4th generation lentiviral packaging system

Fourth-generation lentiviral packaging system. A lentiviral vector and the Lenti-X HTX Packaging Mix are cotransfected into 293T cells. High titer lentiviral supernatants are ready for use 48 hr after transfection.

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Lentiviral vector systems with EF-1 alpha promoters

Lentiviral vector systems with EF-1 alpha promoters. Achieve robust, constitutive, long-term expression of your gene of interest in cell types in which CMV promoters are often silenced, such as hematopoietic and stem cells.

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631253: Lenti-X Expression System (EF1alpha Version)