AAV2 tetracycline-inducible expression system
Tet-One systems provide all necessary components for tetracycline-inducible (tet) expression on a single vector delivered by AAV2 virus. The Tet-On 3G transactivator is expressed from the human phosphoglycerate kinase 1 (PGK) promoter, and the cloned gene of interest is expressed from the PTRE3GS promoter. The AAVpro system contains the reagents to generate the Tet-One vector, as well as components to efficiently package and isolate AAV2 particles.
Tet-One systems provide all necessary components for tetracycline-inducible (tet) expression on a single vector delivered by AAV2 virus. The Tet-On 3G transactivator is expressed from the human phosphoglycerate kinase 1 (PGK) promoter, and the cloned gene of interest is expressed from the PTRE3GS promoter. The AAVpro system contains the reagents to generate the Tet-One vector, as well as components to efficiently package and isolate AAV2 particles. This kit uses helper-free packaging, a unique system for preparing high-titer AAV2 particles without the use of a helper virus.
There are also several other Tet-One vector options for mammalian cells.
Overview
- All-in-one vector design allows you to establish tet-inducible expression of your gene of interest with a single vector
- Low background expression and high induction
- Transactivator expression from the human PGK promoter allows inducible expression in a broad range of tissues
- The unique AAV packaging system allows preparation of high-titer AAV2 without the use of a helper virus
- Kit includes AAV Extraction Solution that increases viral particle recovery as compared to conventional freeze-thaw methods
More Information
Applications
- Tightly controlled, tetracycline-inducible expression of your gene of interest in mammalian cells
References
Heinz, N. et al. Retroviral and transposon-based tet-regulated all-in-one vectors with reduced background expression and improved dynamic range. Hum. Gene Ther. 22, 166–176 (2011).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Tighly controlled expression system that is as easy to use as any constitutive system
Until now, the best inducible mammalian expression systems have all required a two-vector format. Tet-One inducible expression systems are the only commercially available single-vector systems that can deliver a >10,000-fold difference in expression levels between induced and uninduced states.
Tet-One technology overview All inducible systemsTakara Bio USA, Inc.
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