RiboGone - Mammalian—low-input ribosomal RNA removal kit for human, mouse, and rat samples
RiboGone - Mammalian specifically removes ribosomal RNA (rRNA) and mitochondrial RNA (mtRNA) from human, mouse, or rat total RNA samples. RiboGone - Mammalian uses oligo hybridization and RNase H digestion to specifically deplete 5S, 5.8S, 18S, and 28S nuclear rRNA, as well as 12S mtRNA. The kit is designed to work with input RNA of any quality, including degraded material (e.g., FFPE samples). RiboGone - Mammalian is highly suited for sample preparation prior to random-primed cDNA synthesis and RNA-seq or transcriptome analysis applications. When used in tandem with SMART-Seq Total RNA Mid Input or the SMARTer Universal Low Input RNA Kit for Sequencing, RiboGone - Mammalian reduces the frequency of rRNA sequences to <5% of total reads.
Overview
- Effective rRNA removal method for human, mouse, and rat total RNA samples—depletes ≥95% of ribosomal RNA sequences
- Suitable for any quality RNA, including FFPE samples
- Low input working range: 10–100 ng starting material
- Works seamlessly with downstream random-primed cDNA synthesis kits like SMART-Seq Total RNA Mid Input (Cat. # 635048, 635049, or 635050) or SMARTer Universal Low Input RNA Kit for Sequencing (Cat. # 634938 & 634940)
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More Information
Applications
Removal of rRNA from low-input total RNA samples prior to random-primed cDNA synthesis
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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