Caspase assays—colorimetric and fluorescent kits
The ApoAlert Caspase Assay Kits provide a simple and convenient way to detect caspase protease activity using fluorometric or colorimetric methods. The assay kits detect caspase activation by assaying for the cleavage of a fluorescent or a colorimetric substrate. The entire protocol—from collection of cells to fluorometric or colorimetric results—takes only 90 minutes.
The ApoAlert Caspase Assay Kits provide a simple and convenient way to detect caspase protease activity using fluorometric or colorimetric methods. The assay kits detect caspase activation by assaying for the cleavage of a fluorescent or a colorimetric substrate. The entire protocol—from collection of cells to fluorometric or colorimetric results—takes only 90 minutes.
Caspase colorimetric& fluorescent assay kits
The caspase-3 colorimetric assays measure the proteolytic cleavage of the chromophore p-nitroanilide (pNA) by caspase-3. Liberated pNA can be monitored colorimetrically by absorbance at 405 nm.
The caspase-9/6 fluorescent assay detects the shift in fluorescence of 7-amino-4-methoxy coumarin (AMC). Upon cleavage, AMC fluorescence can be measured using a 380-nm excitation filter and a 460-nm emission filter. Fluorescence detection is highly sensitive and can be used to measure even very small amounts of active caspase.
The caspase-3 fluorescent assays detect the shift in fluorescence emission of 7-amino-4-trifluoromethyl coumarin (AFC). After the substrate is cleaved by the protease, AFC emits a yellow-green fluorescence at 505 nm if excited at 400 nm.
Overview
- Measure one of the earliest indicators of apoptosis—caspase proteolytic activity
- Perform the assay directly on cell lysates
- Fast and easy—caspase colorimetric assays and fluorescent assays
- Easily formatted for high-throughput applications
- Works with as few as 1 x 106 adherent cells or cells in suspension
- No substrate handling
More Information
Applications
- Measure caspase activity in cells
- Monitor apoptotic events at a cell population level
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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