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Home › Products › PCR › PCR master mixes › Routine › Takara Taq HS

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Tips and FAQs PCR FAQs

Takara Taq DNA polymerase, hot start

The hot-start versions of TaKaRa Taq DNA Polymerase contain a mixture of Taq polymerase and a monoclonal antibody that binds to Taq polymerase, thereby preventing DNA synthesis at room temperature. During the initial DNA denaturation step (reaction temperature ~94°C), the antibody is denatured, releasing the polymerase and allowing DNA synthesis to proceed. The use of a hot-start PCR enzyme prevents nonspecific amplification due to mispriming and/or the formation of primer dimers during PCR assembly.

Several hot-start versions of Takara Taq are available:

The hot-start versions of TaKaRa Taq DNA Polymerase contain a mixture of Taq polymerase and a monoclonal antibody that binds to Taq polymerase, thereby preventing DNA synthesis at room temperature. During the initial DNA denaturation step (reaction temperature ~94°C), the antibody is denatured, releasing the polymerase and allowing DNA synthesis to proceed. The use of a hot-start PCR enzyme prevents nonspecific amplification due to mispriming and/or the formation of primer dimers during PCR assembly.

Several hot-start versions of Takara Taq are available:

  • TaKaRa Taq DNA Polymerase Hot Start Version—enzyme, buffer, and dNTPs are supplied as separate components
  • Premix Taq DNA Polymerase Hot Start Version—2X premix containing Takara Taq HS enzyme, dNTPs, and an optimized reaction buffer
  • TaKaRa Taq HS Perfect Mix—2X hot-start premix optimized for fast reactions. It contains a modified Taq DNA polymerase, which lacks exonuclease activities, and is compatible with a 20 sec/kb extension step
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Cat. # Product Size Price License Quantity Details
R300B TaKaRa Taq™ HS Perfect Mix 400 Rxns USD $585.00

TaKaRa Taq HS Perfect Mix is a convenient, hot-start 2X PCR master mix that includes DNA polymerase, optimized reaction buffer, and dNTPs. Perfect Mix contains a modified Taq DNA polymerase, which lacks exonuclease activities. It is designed for rapid extension and good PCR specificity. Cat. # R300B contains 4 of Cat. # R003A. Please refer to Cat. # R300A for complete product documentation and resources.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents
R300A TaKaRa Taq™ HS Perfect Mix 100 Rxns USD $147.00

TaKaRa Taq HS Perfect Mix is a convenient, hot-start 2X PCR master mix that includes DNA polymerase, optimized reaction buffer, and dNTPs. Perfect Mix contains a modified Taq DNA polymerase, which lacks exonuclease activities. It is designed for rapid extension and good PCR specificity.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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R300A: TaKaRa Taq HS Perfect Mix

R300A: TaKaRa Taq HS Perfect Mix
R028A Premix Taq™ DNA Polymerase Hot-Start Version 100 Rxns USD $185.00

A convenient 2X PCR master mix which consist of Takara Taq HS polymerase, optimized reaction buffer, and dNTPs. Takara Taq HS polymerase is an antibody-mediated hot-start version of TaKaRa Taq DNA Polymerase, which is a recombinant version of full-length Taq polymerase. Takara Taq has the same characteristics and capabilities as the native Taq polymerase, and is suitable for a variety of standard PCR applications.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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R028A: Premix Taq DNA Polymerase Hot-Start Version

R028A: Premix Taq DNA Polymerase Hot-Start Version
R007B TaKaRa Taq™ DNA Polymerase Hot Start Version 1,000 Units USD $702.00

An antibody-mediated hot-start version of TaKaRa Taq DNA Polymerase, which is a recombinant version of full-length Taq polymerase. It has the same characteristics and capabilities as the native Taq polymerase, and is suitable for a variety of standard PCR applications. The polymerase is supplied with separate tubes of buffer (Mg2+ plus) and dNTPs. Cat. # R007B contains 4 of Cat. # R007A. Please refer to Cat. # R007A for complete product documentation and resources.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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R007B: TaKaRa Taq DNA Polymerase Hot Start Version

R007B: TaKaRa Taq DNA Polymerase Hot Start Version
R007A TaKaRa Taq™ DNA Polymerase Hot Start Version 250 Units USD $195.00

An antibody-mediated hot-start version of TaKaRa Taq DNA Polymerase, which is a recombinant version of full-length Taq polymerase. It has the same characteristics and capabilities as the native Taq polymerase, and is suitable for a variety of standard PCR applications. The polymerase is supplied with separate tubes of buffer (Mg2+ plus) and dNTPs.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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R007A: TaKaRa Taq DNA Polymerase Hot Start Version

R007A: TaKaRa Taq DNA Polymerase Hot Start Version

Primer design FAQs Primer design FAQs
Optimization FAQs Optimization FAQs
Troubleshooting FAQs Troubleshooting FAQs

Overview

Integrated hot-start antibody for enhanced specificity: minimizes primer-dimer formation and reduces background, making it suitable for multiplex PCR. The hot start also allows for convenient reaction setup at room temperature.

More Information

  • Hot-start PCR
  • Multiplex PCR

Bulk, custom, and OEM information

If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


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Tech note

Highly efficient and specific multiplex PCR using TaKaRa Taq DNA Polymerase Hot Start Version

Takara HS Taq demonstrates superior efficiency and specificity over standard Taq Polymerase in this multiplex PCR application. Using Takara HS Taq results in target amplification efficiencies equivalent to those of separate (single target) amplification reactions.

Learn more


pcr success PCR learning center
Selection guides PCR selection guide
Citations Citations: Takara Taq

See what our customers are saying about Takara Taq DNA Polymerase Hot Start Version!

"This is a great product. We love being able to assemble the reactions at room temperature! We also get very little background. Thanks!"
—Gina Riggio, PENN STATE UNIVERSITY
"Product attended expectations. Sales representative is very efficient and prompt to help too!"
—Leticia Soares, UNIVERSITY OF MISSOURI-SAINT LOUIS



Find answers to your PCR questions

Primer design

Frequently asked questions about primer design for successful PCR.

Optimization

Frequently asked questions about PCR optimization.

Troubleshooting

Frequently asked questions about troubleshooting your PCR problems.

Applications and conditions

Frequently asked questions about general and specific applications for PCR and which polymerases to choose.

Shipping, storage, and handling

Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.

Avoid DNA contamination in PCR

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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