Western BLoT Blocking Buffer

Selecting a blocking buffer that best suppresses non-specific signals is critical to obtaining clear, specific Western blot results. The protein-free version is completely chemically defined, has a higher sensitivity than other commonly used blocking agents, such as nonfat milk and is excellent for detecting phosphorylated proteins. The Blocking Buffer (Fish Gelatin) is a reagent of fish origin that is less likely to cross-react with mammalian antibodies than conventional blocking reagents.

Selecting a blocking buffer that best suppresses non-specific signals is critical to obtaining clear, specific Western blot results. The protein free version is completely chemically defined, has a higher sensitivity than other commonly used blocking agents, such as nonfat milk and is excellent for detecting phosphorylated proteins. The Blocking Buffer (Fish Gelatin) is a reagent of fish origin that is less likely to cross-react with mammalian antibodies than conventional blocking reagents.

To obtain the highest possible detection signal for Western blotting, use these products in combination with the Western BLoT HRP Substrate Series for HRP chemiluminescence.

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Cat. #	Product	Size	Price
T7132A	Western BLoT Blocking Buffer (Protein Free)	500 mL	USD $187.00
Selecting a blocking buffer that best suppresses non-specific signals is critical to obtaining clear, specific Western blot results. This product is completely chemically defined and free of protein components. Because of this, the Western BLoT Blocking Buffer (Protein Free) provides higher sensitivity than other commonly used blocking agents, such as nonfat milk. In addition to detection of normal proteins, this blocking buffer is excellent for the detection of phosphorylated proteins. In addition, completely protein-free assays are possible when this product is used in combination with Western BLoT Immuno Booster PF (Cat. #T7115A). To obtain the highest detection sensitivity, combine with the Western BLoT HRP Substrate series. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like
T7131A	Western BLoT Blocking Buffer (Fish Gelatin)	1 L	USD $137.00
The Western BLoT Blocking Buffer (Fish Gelatin) is a blocking buffer that contains gelatin extracted from fish in TBS-T buffer. Since the blocking reagent in this product is of fish origin, it is less likely to cross react with antibodies of mammalian origin than conventional blocking reagents of mammalian origin, such as dry milk and BSA. With the low background obtained with this product, it is possible to clearly detect antigens. This Blocking Buffer is ready-to-use without dilution for blocking Western blot membranes. The Buffer can also be used for dilution of primary antibodies and secondary antibodies labeled with AP or HRP. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back T7131A: Western BLoT Blocking Buffer (Fish Gelatin)

Overview

Low background allows clear detection of antigens
Blocking buffers are ready-to-use without dilution
Can be used for blocking as well as dilution of primary and secondary antibodies
Compatible with secondary antibodies labeled with alkaline phosphatase or horseradish peroxidase

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Applications

Blocking buffer for Western blotting

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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