Western blot signal enhancer: Western BLoT Immuno Booster

Western BLoT Immuno Booster reagents can be used in a variety of immunoassays, including Western blotting and ELISA, to enhance antigen-antibody interactions and thereby increase final detection sensitivity. These products do not affect the activity of horseradish peroxidase (HRP) or alkaline phosphatase (AP), and therefore are compatible with enzyme-conjugated secondary antibodies and with any detection method (i.e., colorimetric or luminescent).

A completely chemically defined version is available for use in protein-free detection systems. Protein-free conditions minimize cross-reactivity that can occur with conventional protein-based blocking buffers, and therefore are ideal for detection of phosphorylated proteins and low-level antigens.

To obtain the highest possible detection signal for Western blotting, use these products in combination with the Western BLoT HRP Substrate Series for HRP chemiluminescence.

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Cat. #	Product	Size	Price
T7115A	Western BLoT Immuno Booster PF	250 mL	USD $307.00
Western BLoT Immuno Booster PF (Protein Free) contains components that enhance antigen-antibody interactions in immunoassays, such as Western blotting, to improve sensitivity and reduce background. This reagent is 100% chemically defined and completely protein-free. The single solution can be used to dilute primary and secondary antibodies and is effective in shortening reaction time, reducing the amount of antibody required, and improving detection of small amounts of antigen. This product does not affect the activity of horseradish peroxidase (HRP) or alkaline phosphatase (AP), and therefore is compatible with enzyme conjugated secondary antibodies and with any detection method (i.e., colorimetric or luminescence). To obtain the highest possible detection signal for Western blotting, use this product in combination with the Western BLoT HRP Substrate Series for HRP chemiluminescence. Because this product is for a protein-free detection system, it is recommended that the Western BLoT Blocking Buffer (Protein Free) (Cat. #T7132A) is used for blocking. Protein-free conditions minimize cross-reactivity that can occur with conventional protein-based blocking buffers, and are therefore ideal for detection of phosphorylated proteins and of low level antigens. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like
T7111A	Western BLoT Immuno Booster	500 mL	USD $347.00
Western BLoT Immuno Booster contains components that enhance antigen-antibody interactions. This product can be used in a variety of immunoassays, including Western blotting and ELISA, to increase the final detection sensitivity (varies from 4-fold to over 10-fold). This product does not effect the activity of horseradish peroxidase (HRP) or alkaline phosphatase (AP), and therefore is compatible with enzyme conjugated secondary antibodies and with any detection method (i.e., colorimetric or luminescence). To obtain the highest possible detection signal for Western blotting, use this product in combination with the Western BLoT HRP Substrate Series for HRP chemiluminescence. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back T7111A: Western BLoT Immuno Booster

Overview

Highly sensitive detection of low abundance proteins without having to change HRP substrate or the amount of antibody
Protein-free version is available

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Applications

Western blot signal enhancer
Increase the final detection sensitivity in immunoassays, such as Western blots and ELISAs

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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