IgG detection without a secondary antibody: Western BLoT Rapid Detect v2.0

Western BLoT Rapid Detect v2.0 is a detection reagent kit for Western blotting that detects primary antibodies using an antibody-free IgG detector solution (HRP-labeled). Compared with conventional detection techniques that use labeled secondary antibodies, this product provides faster, higher sensitivity detection and can be used for simultaneous detection of multiple types of primary antibodies. An added blocking component in the v2 dilution buffer allows for even higher sensitivity and simpler use.

The main component of this product is the IgG detector, an HRP conjugate that binds IgG. On the surface of one molecule of IgG Detector there are approximately 100 antibody-binding protein molecules and approximately 50 molecules of HRP.

The performance of this kit has been validated in combination with the Western BLot HRP chemiluminescent substrate series.

Cat. #	Product	Size	Price	License	Quantity	Details
T7122A	Western BLoT Rapid Detect v2.0	50 Rxns	USD $663.00
Western BLoT Rapid Detect v2.0 is a Western blot detection reagent kit used to detect primary antibodies using an HRP-labeled IgG Detector instead of a labeled secondary antibody. IgG Detector, the main component of this product, is a particle with approximately 100 protein molecules on its surface that bind to the Fc region of an antibody, and is labeled with about 50 molecules of HRP. This reagent enables quick, high-sensitivity detection and can be used for simultaneous detection of multiple primary antibodies. Moreover, the use of the included Dilution Buffer, which contains a blocking component, allows for higher sensitivity and simpler use. For the highest sensitivity and quickest detection for Western blotting, use this product in combination with the Western BLoT HRP Substrate series. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back T7122A: Western BLoT Rapid Detect v2.0

Overview

Simultaneous detection of multiple primary antibodies
Detection of minute quantities of antigen that cannot be detected using conventional techniques with a labeled secondary antibody

More Information

Applications

Western blot signal detection without using a labeled secondary antibody
Secondary antibody enhancement: IgG detector can be used after conventional techniques (labeled secondary antibody) to intensify the signal
Re-probing blots that produced a weak signal with conventional techniques (labeled secondary antibody)

Antibody compatibility

This product can detect most primary antibodies with the exception of a few, such as goat IgG. If the primary antibody is a goat IgG, this product cannot be used. However, this kit can be used for secondary antibody enhancement as described in the User Manual.

If the primary antibody is mouse IgG, the IgG Detector has low binding capacity. For detection of mouse IgG, add the Enhancer for Mouse IgG to the IgG Detector.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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