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Yeast two-hybrid media sets

Our yeast two-hybrid media sets contain all of the yeast media pouches you will need for the Matchmaker Gold Yeast Two-Hybrid System protocols. The media is sold aliquoted in highly convenient pouches. Simply empty the contents of each pouch into your media flask/bottle, add 0.5 L of dH₂0, and sterilize. No weighing, combining, or pH adjustment is required. The Yeast Media Set 2 Plus also contains Aureobasidin A and our largest aliquot of X-alpha-Gal, two reagents that are integral to the Matchmaker Gold Protocol.

Cat. #	Product	Size	Price
630495	Yeast Media Set 2 Plus	Each	USD $775.00
The Yeast Media Set 2 Plus contains the media and supplements needed for yeast two-hybrid library screening with the Matchmaker Gold Yeast Two-Hybrid System (Cat. No. 630489). For the ready-to-go, preformulated pouches, just add water and autoclave according to the instructions provided in the Yeast Media Protocol-at-a-Glance: no measuring, mixing, or pH adjustments are required. Directions for the use of Aureobasidin A and X-alpha-Gal are provided in the Matchmaker Gold Yeast Two-Hybrid System User Manual. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back The yeast two-hybrid principle The yeast two-hybrid principle. A bait protein interacts with the GAL4 recognition sequence (or promoter) upstream of a reporter gene. Transcription of the reporter is activated when a prey protein containing the GAL4 transcriptional activation domain interacts with the bait. Back Matchmaker Gold reporter genes Matchmaker Gold reporter genes. Yeast strain Y2HGold expresses 4 genes from 3 separate GAL4-responsive promoters in response to protein-protein interactions. Back Yeast media pouches Yeast media pouches. Each ready-to-go pouch provides a precise amount of premixed media and supplements to make 0.5 L of broth or agar media. Yeast Media Sets contain a complete assortment of mixes for preparing eight specialized broth and agar media, and are designed to complement our advanced Matchmaker Gold Systems. The Yeast Media Set 2 Plus includes Aureobasidin A and X-alpha-Gal, as well as media.
630494	Yeast Media Set 2	Each	USD $365.00
Complete media sets for two-hybrid screening. Highest performing yeast media-hybrid system. Package Contents: 2 pouches YPDA Broth 1 pouch YPDA with Agar 1 pouch SD/-Leu Broth 1 pouch SD/-Leu with Agar 1 pouch SD/-Trp Broth 1 pouch SD/-Trp with Agar 10 pouches SD/-Leu/-Trp with Agar 1 pouches SD/-Ade/-His/-Leu/-Trp with Agar Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Yeast media pouches Yeast media pouches. Each ready-to-go pouch provides a precise amount of premixed media and supplements to make 0.5 L of broth or agar media. Yeast Media Sets contain a complete assortment of mixes for preparing eight specialized broth and agar media, and are designed to complement our advanced Matchmaker Gold Systems. The Yeast Media Set 2 Plus includes Aureobasidin A and X-alpha-Gal, as well as media. Back 630494: Yeast Media Set 2

Overview

Complete media sets for two-hybrid screening
Convenient; all you need for Matchmaker Gold systems in a single package
Extra value; cheaper than purchasing the media separately
Simply mix each pouch with 500 ml water and sterilize

More Information

Applications

Yeast two-hybrid screening (Matchmaker Gold)

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Yeast two-hybrid media sets

Notice to purchaser

The yeast two-hybrid principle

Matchmaker Gold reporter genes

Yeast media pouches