Yeast strains for yeast two-hybrid screening

We offer two highly specialized yeast strains, Y2HGold and Y187, that are optimized for exceptionally stringent two-hybrid library screening and two-hybrid library construction, respectively.

Y2HGold Yeast Strain

Y2HGold plays a crucial role in establishing the high stringency of our Matchmaker Gold Yeast Two-Hybrid System due to its four integrated reporter genes (AUR1-C, ADE2, HIS3, and MEL1), which are under the control of three distinct Gal4-responsive promoters.

The Matchmaker Gold Yeast Two-Hybrid System is the only yeast two-hybrid system with four genuine reporters and three different bait recognition sequences. Background growth is not possible due to the potency and stability of Aureobasidin A, a novel yeast two-hybrid antibiotic that kills all non-resistant cells. False positives are reduced to a minimum because any independently binding prey protein would need to bind to all three recognition sequences and activate four different reporters.

Y187 Yeast Strain

Y187 is used in the construction of our Mate & Plate and normalized Mate & Plate Libraries, which are recommended for use with the Matchmaker Gold Yeast Two-Hybrid System. These complex, tissue-specific, ready-to-go libraries are constructed directly in our Y187 library yeast stain, utilizing the highly efficient homologous recombination machinery of Saccharomyces cerevisiae. Mate & Plate Libraries provide, by far, the simplest method for yeast two-hybrid screening because they do not require library-scale transformations or labor-intensive library amplification.

You can also use the Y187 Yeast Strain with our convenient Make Your Own "Mate & Plate" Library System to construct your own yeast two-hybrid libraries, and eliminate the time-consuming and labor-intensive library cloning, amplification, and harvesting in E. coli that are required by traditional methods of library construction.