BcaBEST DNA Polymerase ver.2.0

BcaBEST DNA Polymerase ver.2.0 provides a robust, easy solution for rapid, point-of-care pathogen detection via loop-mediate isothermal amplification (LAMP), RT-LAMP, or other isothermal amplification techniques. Originating from the thermophilic bacteria Bacillus caldotenax, BcaBEST DNA Polymerase ver.2.0 has been engineered to enhance the activity of the DNA polymerase without compromising the enzyme’s strong strand-displacement activity, resulting in increased speed and sensitivity.

Simply set up your reaction, incubate at 60–65°C for 30 min, and employ your detection method of choice to identify samples containing your desired nucleic acid sequence. BcaBEST DNA Polymerase ver.2.0 is compatible with fluorescent monitoring on a real-time PCR machine and various colorimetric/visual detection methods.

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Cat. #	Product	Size	Price
RR380A	BcaBEST™ DNA Polymerase ver.2.0	1,600 U	USD $79.00
BcaBest DNA Polymerase ver 2.0 is a strand-displacing DNA polymerase that has been engineered for increased speed and sensitivity, making it ideal for LAMP, RT-LAMP, and other isothermal PCR applications. BcaBest DNA Polymerase ver.2.0 (8 U/µl) and 2X BcaBEST Buffer containing dNTPs are included. LAMP primers corresponding to a desired nucleic acid sequence are not included and must be provided by the user. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back RR380A: BcaBEST DNA Polymerase ver.2.0 Back Sensitivity and specificity of BcaBEST DNA Polymerase ver.2.0 by real-time fluorescent detection *Sensitivity and specificity of BcaBEST DNA Polymerase ver.2.0 by real-time fluorescent detection.* BcaBEST DNA Polymerase ver.2.0 was used in a LAMP assay to amplify the fecA gene from 10 pg (blue) or 100 pg (green) of JM109 genomic DNA, and reactions were monitored on a real-time PCR machine. Using BcaBEST DNA Polymerase Ver.2.0 resulted in the amplification of both the 10 pg and 100 pg samples, while no amplification was seen in the no template control sample (NTC; purple). Back Sensitivity of BcaBEST DNA Polymerase ver.2.0 by colorimetric detection *Sensitivity of BcaBEST DNA Polymerase ver.2.0 by colorimetric detection. Samples spiked with 0, 5, 500, 5,000, or 50,000 copies of the SARS-CoV-2 N RNA transcript were used in RT-LAMP reactions using Bca*BEST DNA Polymerase ver.2.0. Calcein (top) or hydroxynaphthol blue (HBN; bottom) were added to the reactions prior to amplification to allow for visualization of the RT-LAMP reaction product. For calcein, a color change from pink-orange to clear-yellow is indicative of detection, while a color change from indigo to light blue is indicative of detection for HNB. For both colorimetric methods, as little as 50 RNA copies were detected.
RR380B	BcaBEST™ DNA Polymerase ver.2.0	8,000 U	USD $339.00
BcaBest DNA Polymerase ver 2.0 is a strand-displacing DNA polymerase that has been engineered for increased speed and sensitivity, making it ideal for LAMP, RT-LAMP, and other isothermal PCR applications. BcaBest DNA Polymerase ver.2.0 (8 U/µl) and 2X BcaBEST Buffer containing dNTPs are included. LAMP primers corresponding to a desired nucleic acid sequence are not included and must be provided by the user. Cat. # RR380B contains 5 of Cat. # RR380A. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Sensitivity and specificity of BcaBEST DNA Polymerase ver.2.0 by real-time fluorescent detection *Sensitivity and specificity of BcaBEST DNA Polymerase ver.2.0 by real-time fluorescent detection.* BcaBEST DNA Polymerase ver.2.0 was used in a LAMP assay to amplify the fecA gene from 10 pg (blue) or 100 pg (green) of JM109 genomic DNA, and reactions were monitored on a real-time PCR machine. Using BcaBEST DNA Polymerase Ver.2.0 resulted in the amplification of both the 10 pg and 100 pg samples, while no amplification was seen in the no template control sample (NTC; purple). Back Sensitivity of BcaBEST DNA Polymerase ver.2.0 by colorimetric detection *Sensitivity of BcaBEST DNA Polymerase ver.2.0 by colorimetric detection. Samples spiked with 0, 5, 500, 5,000, or 50,000 copies of the SARS-CoV-2 N RNA transcript were used in RT-LAMP reactions using Bca*BEST DNA Polymerase ver.2.0. Calcein (top) or hydroxynaphthol blue (HBN; bottom) were added to the reactions prior to amplification to allow for visualization of the RT-LAMP reaction product. For calcein, a color change from pink-orange to clear-yellow is indicative of detection, while a color change from indigo to light blue is indicative of detection for HNB. For both colorimetric methods, as little as 50 RNA copies were detected. Back RR380B: BcaBEST DNA Polymerase ver.2.0

Overview

Improved sensitivity compared to BcaBEST DNA Polymerase
Reaction completes in 30 min or less
High thermostability
Compatible with real-time fluorescent monitoring and colorimetric/visual detection methods

BcaBEST DNA Polymerase ver.2.0 amplifies nucleic acids faster without sacrificing specificity

	BcaBEST ver.2.0	Vendor N ver.2.0	Vendor N ver.3.0	Vendor Y	Vendor V
NTC	–	44.35	24.25	35.5	–
10 pg	16.6	31.9	20.8	33.85	57.4
100 pg	14.9	28.1	13.85	28.25	55.9

Figure 1. Average Ct values obtained from LAMP reactions using commercially available DNA polymerases. Commercially available DNA polymerases that are advertised as appropriate for LAMP were used to amplify the fecA gene from JM109 genomic DNA, and reactions were monitored on a real-time PCR machine. Average Ct values for the no template control (NTC), reactions using 10 pg of genomic DNA, and reactions using 100 pg of genomic DNA are shown for each polymerase. LAMP reactions using BcaBEST ver.2.0 resulted in the lowest Ct values for the 10 pg and 100 pg reactions, which is indicative of the fastest reaction time, without amplification in the NTC reaction.

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Applications

LAMP, RT-LAMP, and other isothermal amplification techniques
Rapid, point-of-care pathogen detection
Low-resource field diagnostics

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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BcaBEST DNA Polymerase ver.2.0

Notice to purchaser

RR380A: BcaBEST DNA Polymerase ver.2.0

Sensitivity and specificity of BcaBEST DNA Polymerase ver.2.0 by real-time fluorescent detection

Sensitivity of BcaBEST DNA Polymerase ver.2.0 by colorimetric detection