TB Green Premix Ex Taq II FAST qPCR
This product is a reagent exclusively for real-time PCR using TB Green, an intercalating dye. The optimized composition combines a mutant Taq DNA polymerase suitable for high-speed reactions and a primer-dimer suppressor to achieve a wide dynamic range due to high fluorescence intensity while maintaining outstanding high-speed reactions and ultimate specificity. High quantification and detection are possible.
This product is a reagent exclusively for real-time PCR using TB Green, an intercalating dye. The optimized composition combines a mutant Taq DNA polymerase suitable for high-speed reactions and a primer-dimer suppressor to achieve a wide dynamic range due to high fluorescence intensity while maintaining outstanding high-speed reactions and ultimate specificity. High quantification and detection are possible.
The heat-stable Tli RNaseH and UNG (uracil-N-glycosylase) contained in the reagent can minimize PCR reaction inhibition of mRNA remaining in the cDNA template and prevent amplification due to carryover contamination. Additionally, the reagent contains a universal dye and a visualization dye, making it much easier to use successfully.
When the sample has a low concentration, use the included sample diluent EASY Dilution II (for Real Time PCR) for more accurate quantitation than dilution with water or TE.
Overview
- Ultimate specificity—unique reaction solution composition suppresses non-specific amplification
- High-speed reaction—made possible by using mutant DNA polymerase
- Improved visibility—implements a tinted reaction solution
- Premix includes:
- Universal Reference Dye—no need to adjust each device
- UNG (uracil-N-glycosylase)—prevents carryover contamination
- Kit includes EASY Dilution II (for Real Time PCR)—allows for more accurate quantification
Schematic of intercalating-dye-based qPCR. Learn more about TB Green-based qPCR kits.
More Information
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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