CellAmp Direct TB Green RT-qPCR Kit
The CellAmp Direct TB Green RT-qPCR Kit is designed for performing a simple two-step real-time RT-PCR, without RNA extraction, directly from various animal cells (e.g., adherent cells, floating cells, primary cells, various stem cells, and iPS cells). The kit makes it possible to prepare template samples from cultured cells and complete the analysis of reverse transcription reactions and gene expression in as little as 1.5 hr.
The CellAmp Direct TB Green RT-qPCR Kit is designed for performing a simple two-step real-time RT-PCR, without RNA extraction, directly from various animal cells (e.g., adherent cells, floating cells, primary cells, various stem cells, and iPS cells). The kit makes it possible to prepare template samples from cultured cells and complete the analysis of reverse transcription reactions and gene expression in as little as 1.5 hr.
This product allows the effective elimination of genomic DNA. Therefore, it works well for analyses in which gDNA contamination is an issue, including situations in which the design of a primer pair spanning an exon junction is not possible or in which low-expression genes are to be analyzed. A two-step real-time RT-PCR reagent for TB Green detection is included in the kit, eliminating the need for reverse-transcription and PCR mixes to be prepared separately. This makes gene expression analysis easy to perform.
Overview
- High-throughput analyses can be performed easily and rapidly (~1.5 hr)—there is no need to do RNA extraction or a heat-treatment to inactivate DNase, making the process faster and less cumbersome
- Can be used with cells differentiated from stem cells and iPS cells—the improved lysis buffer works well with a variety of adherent cells, floating cells, primary cultured cells, various stem cells, iPS cells, etc.
- Lysates can be stored for a long time—storage stability has been confirmed at –20°C for at least 6 months
- Resistance to PCR inhibitors and high specificity—use of TB Green Fast qPCR Mix as the real-time PCR reagent makes this product highly resistant to PCR inhibitors and highly reactive for high GC-content targets
More Information
Applications
- High-throughput gene expression analyses in a very short time (~1.5 hr)
- RT-qPCR of stem cells (such as iPS cells, floating cells, adherent cells, primary cells, etc.)
- Analysis of samples with high GC content-high tolerance to inhibitors and high specificity
- Analysis of samples typically impacted by genomic DNA contamination
Components
| 3735A (100 reactions) | 3735S (20 reactions) | |
| CellAmp Washing Buffer | 2.5 ml x 5 | 2.5 ml |
| CellAmp Lysis Buffer II | 1 ml x 5 | 1 ml |
| DNase I for Direct RNA Prep | 200 μl | 40 μl |
| Stop Solution | 250 μl | 50 μl |
| PrimeScript RT Enzyme Mix | 200 μl | 40 μl |
| 5X CellAmp Buffer II | 400 μl | 80 μl |
| RT Primer Mix | 100 μl | 20 μl |
| RNase Free H2O | 1 ml x 2 | 1 ml |
| TB Green Fast qPCR Mix (2X) (3735A/S) | 625 μl x 10 | 625 μ x 2 |
| ROX Reference Dye (50X) | 250 μl | 50 μl |
| ROX Reference Dye II (50X) | 250 μl | 50 μl |
Storage
–20°C
Additional Product Information
Please see the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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