Prelude One-Step PreAmp Master Mix—high-quality preamplification of limited RNA samples

Prelude One-Step PreAmp Master Mix uses an optimized reverse transcriptase, polymerase, and buffer to maximize RT-PCR efficiency for all targets in a preamplification reaction. This enables the highest level of unbiased preamplification for >100 targets, from as little as 100 pg mRNA or 10 ng total RNA. This 2X premix works with intact RNA or degraded RNA extracted from FFPE samples. Amplified products can be used for downstream real-time PCR (qPCR), genotyping, or target-enrichment analysis.

qPCR, SNP genotyping, and targeted enrichment are powerful molecular techniques for a wide range of research and clinical applications. However, these techniques may be less effective when working with limited or precious starting materials, such as nucleic acids extracted from FFPE tissue, samples obtained in extremely small quantities, or material that occurs at dilute concentrations in biofluids.

These challenges can be overcome using preamplification (originally called booster PCR), which enables unbiased, target-specific amplification of cDNA and gDNA. The key consideration when performing preamplification is ensuring that your preamplification mix is sensitive enough to work with low-input amounts of template, while not introducing bias. Bias can occur when PCR efficiency varies for some of the targets and results in their under- or over-representation in the preamplified sample, which can then influence the data generated by downstream assays. Prelude One-Step PreAmp Master Mix allows unparalleled sensitivity and efficiency, ensuring the even amplification of many targets from very low RNA input amounts and giving you confidence in your results.