Directed differentiation

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Cellartis Human Pluripotent Stem Cell Services

Directed differentiation to hepatocytes

We have over 20 years of experience differentiating pluripotent cells along the endodermal lineage. Using a proprietary protocol that mimics normal embryonic development, your pluripotent cells will be differentiated into high-quality hepatocytes.

For more information about our services, please submit the service inquiry form below.

Overview Convenient options Deliverables/lead time QC testing Project initiation

Overview

With the Cellartis Directed Differentiation to Hepatocytes Service, we deliver high-quality, custom hepatocytes derived from your human ES or iPS cells. As an alternative to providing your own cells, you may request Cellartis sourcing and reprogramming services.

Our differentiation protocols start with thawing and adapting your pluripotent cells to the Cellartis DEF-CS 500 Culture System, which maintains cells in a highly pluripotent and homogeneous state. After adaptation, cells are expanded to generate a Seed Bank. The pluripotent cells are then differentiated into hepatocytes, banked, and characterized.

Thawing of your ES or iPS cells and adaptation to the DEF-CS system

Expansion of the cells in the DEF-CS system and generation of a Seed Bank

Differentiation into definitive endoderm cells

Differentiation into hepatocytes and banking

Characterization of the banked, final cell product (QC testing included)

Convenient options

Our standard service includes the delivery of 30 vials of human iPS or ES cell-derived hepatocytes. To meet your requirements for early evaluation, we can also provide a small-scale delivery service of 5 vials.

Deliverables/lead time

Service	Cat. #	Deliverables	Lead time
Cellartis hiPS Directed Differentiation to Hepatocytes Service (5 vials)	Y60222	Project report 5 vials of hepatocytes (12 million viable cells per vial)	Approx. 22 weeks
Cellartis hES Directed Differentiation to Hepatocytes Service (5 vials)	Y60223	Project report 5 vials of hepatocytes (12 million viable cells per vial)	Approx. 22 weeks
Cellartis hiPS Directed Differentiation to Hepatocytes Service (30 vials)	Y60220	Project report 30 vials of hepatocytes (12 million viable cells per vial)	Approx. 22 weeks
Cellartis hES Directed Differentiation to Hepatocytes Service (30 vials)	Y60221	Project report 30 vials of hepatocytes (12 million viable cells per vial)	Approx. 22 weeks
Cellartis hiPS Directed Differentiation to Hepatocytes Service (Add-On, 30 vials) (available after initial purchase of 5- or 30-vial option)	Y60224	Project report Add-On, 30 vials of hepatocytes (12 million viable cells per vial)	Approx. 16 weeks
Cellartis hES Directed Differentiation to Hepatocytes Service (Add-On, 30 vials) (available after initial purchase of 5- or 30-vial option)	Y60225	Project report Add-On, 30 vials of hepatocytes (12 million viable cells per vial)	Approx. 16 weeks

QC testing

Included with the hepatocyte directed differentiation services:

Visual inspection of hepatocyte morphology
Viability (>12 million viable cells per vial)
Immunocytochemistry to verify the hepatocyte phenotype: HNF4α (+)
Sterility testing (including Mycoplasma)

Additional testing and characterization services for hepatocytes:

Cellartis Cytochrome P450 Activity Profiling Service (Cat. #Y60307)
- Measurement of cytochrome P450 enzyme activity, including CYP1A, 3A, 2C9, 2B6, 2D6, and 2C9

Cellartis Hepatic Gene Expression Profiling Service (Cat. #Y60308)
- qRT-PCR analysis for mature hepatocyte markers, including albumin, CYP3A4/5/7, 2C9, 2C19, 2D6, 2B6, and HNF4α

Cellartis Hepatic Protein Expression Profiling Service (Cat. #Y60309)
- Immunostaining for A1AT, albumin, CK18, and GSTA1

Project initiation

Successful differentiation depends on the quality of the starting material; a homogeneous, undifferentiated stem cell population is ideal. To get started, we will need 5 vials (2.5 million cells per vial) of customer-provided hiPS or hES cells, with relevant information regarding, including name of the donor cell line, culture conditions, and cell density upon freezing. Your cells will be adapted to the DEF-CS system, and then the differentiation protocol can begin.

For safety and QC purposes, we can only work with human samples that are free of human pathogens and that have passed a sterility test. We require documentation proving that all samples shipped to us are sterile and pathogen-free. In the absence of documentation, testing for pathogens (Cat. # Y60301) and sterility (Cat. # Y60302) will be performed via a third-party service prior to the initiation of Cellartis services. Please note that safety and sterility testing will incur additional lead time and costs.

Submit the service inquiry form below.
We contact you to go over your project requirements and provide a quote.
Place your order and ship us the necessary cells and documentation to initiate the service.
We perform the agreed-upon service according to the designated lead times.
We provide you with the agreed-upon deliverables.

Note: Takara Bio Europe, AB (TBEAB) will perform the differentiation service only one time per purchase. Each purchase of the differentiation service includes protocol optimization to determine whether or not successful differentiation of the Customer’s iPS or ES cell line can be achieved. If a successful differentiation cannot be achieved after the performance of the protocol optimization, the differentiation service will be terminated and neither TBUSA nor TBEAB will have any further obligation, including additional attempts of the differentiation service. If additional work is required, TBUSA will discuss such work, pricing, and lead time with the customer.

Hepatocyte differentiation services

Cat. #

Product

Size

Price

License

Quantity

Details

Y60220

Cellartis® hiPS Directed Differentiation to Hepatocytes Service (30 vials)

Each

Inquire for Quotation

License Statement

ID Number

L69

This product is covered by one or more claims of the issued patents and pending patent applications: U.S. Patent Nos. 8,048,999, 8,058,065, 8,129,187, 8,211,697, 8,257,941, 8,278,104, 8,530,238, 8,900,871, 8,927,277, 8,951,801, 9,213,999, 9,404,124, 9,499,797, 9,677,141, 9,714,433, and 9,938,585 and foreign counterparts thereof, including their divisions, continuations, extensions, substitutions, and those claiming priority therefrom or those claiming the same priorities therein.

This custom service is provided by Takara Bio Inc. and/or its affiliate(s) based on a patent license from iPS Academia Japan, Inc. No express or implied license is granted to the purchaser. "Product" means the customized differentiated cells manufactured and sold by Takara Bio Inc.

"Internal research purpose" means the use of this Product as a tool of internal research for the sole benefit of the purchaser or purchaser's contract research customer, (including screening of compounds, antibodies, proteins, peptides and others in developing pharmaceuticals (including diagnostic agents) for human and/or animal use), provided, however, that the following activities using this Product are strictly prohibited and expressly excluded from Internal research purposes:

With the exemption for academic and non-commercial clinical research done by non-profit institutions;

1) Administration of this Product to humans.

2) Use of this Product for the diagnostic testing, prevention, or treatment of human and/or animal diseases.

3) Use of this Product in studies involving administration to humans for cell therapy, regenerative medicine etc.

Purchaser may use this Product for its own Internal research purposes.

Purchaser shall not use the Products for its commercial purposes.

Notwithstanding the foregoing, the purchaser of this Product may provide third parties with contract research services by using this Product only for such third party's Internal research purposes.

With Cellartis directed differentiation services, we will deliver high-quality, custom hepatocytes or definitive endoderm cells derived from your human ES or iPS cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Y60221

Cellartis® hES Directed Differentiation to Hepatocytes Service (30 vials)

Each

Inquire for Quotation

License Statement

ID Number

C015

This service is performed by Takara Bio Europe SAS based on a commercial license to certain intellectual property rights held by Wisconsin Alumni Research Foundation (“WARF”). The deliverables and their use are covered by one or more claims of U.S. Patent No. 7,514,260 and its foreign counterparts. The purchase of this service conveys to the buyer the non-transferable right to use the deliverables for their intended use, strictly limited to purchaser’s own internal research. No other express or implied license is granted to the purchaser. Purchaser cannot have any right to use the deliverables or its components in humans for any purposes including but not limited to diagnostics and/or therapeutics, or otherwise clinical trials. Purchase does not include any right to resell or transfer the deliverables to a third party regardless of whether or not compensation is received. Purchasers wishing to use the deliverables for purposes other than internal research use should contact us.

With Cellartis directed differentiation services, we will deliver high-quality, custom hepatocytes or definitive endoderm cells derived from your human ES or iPS cells.

Notice to purchaser

Y60222

Cellartis® hiPS Directed Differentiation to Hepatocytes Service (5 vials)

Each

Inquire for Quotation

License Statement

ID Number

L69

With the Cellartis hiPS directed differentiation to hepatocytes service, we will deliver high-quality, custom hepatocytes derived from your human iPS cells.

Notice to purchaser

Y60223

Cellartis® hES Directed Differentiation to Hepatocytes Service (5 vials)

Each

Inquire for Quotation

License Statement

ID Number

C015

With the Cellartis hES directed differentiation to hepatocytes service, we will deliver high-quality, custom hepatocytes derived from your human ES cells.

Notice to purchaser

Y60224

Cellartis® hiPS Directed Differentiation to Hepatocytes Service (Add-On, 30 vials)

Each

Inquire for Quotation

License Statement

ID Number

L69

With the Cellartis hiPS directed differentiation to hepatocytes service, we will deliver high-quality, custom hepatocytes derived from your human iPS cells.

Notice to purchaser

Y60225

Cellartis® hES Directed Differentiation to Hepatocytes Service (Add-On, 30 vials)

Each

Inquire for Quotation

License Statement

ID Number

C015

With the Cellartis hES directed differentiation to hepatocytes service, we will deliver high-quality, custom hepatocytes derived from your human ES cells.

Notice to purchaser

Y60101

Cellartis® Sendai Virus Reprogramming of Fibroblasts Service

Each

Inquire for Quotation

License Statement

ID Number

L62

This product is covered by one or more claims of the issued patents and pending patent applications: U.S. Patent Nos. 8,048,999, 8,058,065, 8,129,187, 8,211,697, 8,257,941, 8,278,104, 8,530,238, 8,900,871, 8,927,277, 8,951,801, 9,213,999, 9,404,124, 9,499,797, 9,677,141, 9,714,433, and 10,385,407 and foreign counterparts thereof, including their divisions, continuations, extensions, substitutions, and those claiming priority therefrom or those claiming the same priorities therein.

This custom service is provided by Takara Bio Inc. based on a patent license from iPS Academia Japan, Inc. The use of iPSCs produced by this service (hereinafter referred to as the "custom iPSCs") is strictly limited to customer’s own internal research. Customer cannot have any right to use the custom iPSCs in humans for any purposes including but not limited to diagnostics and/or therapeutics, or otherwise clinical trials. There is no right to resell or transfer the custom iPSCs to a third party regardless of whether or not compensation is received.

The use of custom iPSCs is not allowed for the purposes of the intended use as described below: 1) Creation of a human clone through transplantation of the custom iPSCs-derived embryos into human or animal individuals, 2) Custom iPSCs transplantation into human embryos, 3) Custom iPSCs transplantation into human fetuses, and 4) Production of human embryos using the custom iPSCs-derived germ cells.

Customers wishing to use the custom iPSCs for purposes other than internal research use should contact us. No express or implied license is granted to the customer. Any use of the custom iPSCs for Commercial Purposes requires a patent license from iPS Academia Japan, Inc. "Commercial Purposes" means at least one of following activities including the use of custom iPSCs: 1) Use for manufacture of related products for sale including but not limited to culture medium or equipment, 2) Use in order to provide a service to a third party (including use of the differentiated cells from the custom iPSCs), 3) Use for screening compounds, antibodies, proteins, peptides and others in developing pharmaceuticals (including diagnostic agents) for human and/or animal use (including use of the differentiated cells from the custom iPSCs)*, and 4) Transfer to a third party (including transfer of the differentiated cells from the custom iPSCs). *A patent license to use the custom iPSCs and the use of the differentiated cells from the custom iPSCs for screening compounds may also be granted from Takara Bio Inc.

L71

This service is performed by Takara Bio Inc. and/or its affiliate(s) based on a commercial license to certain intellectual property rights held by ID Pharma Co.,Ltd. The deliverables and their use are covered by one or more claims of patents owned by ID Pharma, including PCT/JP00/03195, PCT/JP2009/062911, PCT/JP2011/069588 and PCT/JP2014/75248 and their foreign counterparts. The purchase of this service conveys to the buyer the non-transferable right to use the deliverables for their intended use, strictly limited to purchaser’s own internal research. No other express or implied license is granted to the purchaser. Purchaser cannot have any right to use the deliverables or its components in humans for any purposes including but not limited to diagnostics and/or therapeutics, or otherwise clinical trials. Purchase does not include any right to resell or transfer the deliverables to a third party regardless of whether or not compensation is received. Purchase does not include any right to provide Contract Manufacturing Service with the use of the deliverables to a third party, unless a license is negotiated with ID Pharma Co., Ltd. Purchasers wishing to use the deliverables for purposes other than internal research use should contact us.

With Cellartis reprogramming services, we will deliver high-quality human iPS cell lines from your fibroblast or PBMC samples.

Notice to purchaser

Y60102

Cellartis® Sendai Virus Reprogramming of PBMCs Service

Each

Inquire for Quotation

License Statement

ID Number

L62

This product is covered by one or more claims of the issued patents and pending patent applications: U.S. Patent Nos. 8,048,999, 8,058,065, 8,129,187, 8,211,697, 8,257,941, 8,278,104, 8,530,238, 8,900,871, 8,927,277, 8,951,801, 9,213,999, 9,404,124, 9,499,797, 9,677,141, 9,714,433, and 10,385,407 and foreign counterparts thereof, including their divisions, continuations, extensions, substitutions, and those claiming priority therefrom or those claiming the same priorities therein.

This custom service is provided by Takara Bio Inc. based on a patent license from iPS Academia Japan, Inc. The use of iPSCs produced by this service (hereinafter referred to as the "custom iPSCs") is strictly limited to customer’s own internal research. Customer cannot have any right to use the custom iPSCs in humans for any purposes including but not limited to diagnostics and/or therapeutics, or otherwise clinical trials. There is no right to resell or transfer the custom iPSCs to a third party regardless of whether or not compensation is received.

The use of custom iPSCs is not allowed for the purposes of the intended use as described below: 1) Creation of a human clone through transplantation of the custom iPSCs-derived embryos into human or animal individuals, 2) Custom iPSCs transplantation into human embryos, 3) Custom iPSCs transplantation into human fetuses, and 4) Production of human embryos using the custom iPSCs-derived germ cells.

Customers wishing to use the custom iPSCs for purposes other than internal research use should contact us. No express or implied license is granted to the customer. Any use of the custom iPSCs for Commercial Purposes requires a patent license from iPS Academia Japan, Inc. "Commercial Purposes" means at least one of following activities including the use of custom iPSCs: 1) Use for manufacture of related products for sale including but not limited to culture medium or equipment, 2) Use in order to provide a service to a third party (including use of the differentiated cells from the custom iPSCs), 3) Use for screening compounds, antibodies, proteins, peptides and others in developing pharmaceuticals (including diagnostic agents) for human and/or animal use (including use of the differentiated cells from the custom iPSCs)*, and 4) Transfer to a third party (including transfer of the differentiated cells from the custom iPSCs). *A patent license to use the custom iPSCs and the use of the differentiated cells from the custom iPSCs for screening compounds may also be granted from Takara Bio Inc.

L71

With Cellartis reprogramming services, we will deliver high-quality human iPS cell lines from your fibroblast or PBMC samples.

Notice to purchaser

Y60301

Cellartis® Human Pathogen and Mycoplasma Testing Service

Each

Inquire for Quotation

With Cellartis directed differentiation services, we will deliver high-quality, custom hepatocytes or definitive endoderm cells derived from your human ES or iPS cells.

Notice to purchaser

Y60302

Cellartis® Sterility Testing Service

Each

Inquire for Quotation

With Cellartis directed differentiation services, we will deliver high-quality, custom hepatocytes or definitive endoderm cells derived from your human ES or iPS cells.

Notice to purchaser

Y60304

Cellartis® Descriptive Karyotyping Service

Each

Inquire for Quotation

With Cellartis reprogramming services, we will deliver high-quality human iPS cell lines from your fibroblast or PBMC samples.

Notice to purchaser

Y60305

Cellartis® Spontaneous In Vitro Differentiation Service

Each

Inquire for Quotation

With Cellartis reprogramming services, we will deliver high-quality human iPS cell lines from your fibroblast or PBMC samples.

Notice to purchaser

Y60306

Cellartis® STR Analysis Service

Each

Inquire for Quotation

With Cellartis reprogramming services, we will deliver high-quality human iPS cell lines from your fibroblast or PBMC samples.

Notice to purchaser

Y60307

Cellartis® Cytochrome P450 Activity Profiling Service

Each

Inquire for Quotation

With Cellartis directed differentiation services, we will deliver high-quality, custom hepatocytes or definitive endoderm cells derived from your human ES or iPS cells.

Notice to purchaser

Y60308

Cellartis® Hepatic Gene Expression Profiling Service

Each

Inquire for Quotation

With Cellartis directed differentiation services, we will deliver high-quality, custom hepatocytes or definitive endoderm cells derived from your human ES or iPS cells.

Notice to purchaser

Y60309

Cellartis® Hepatic Protein Expression Profiling Service

Each

Inquire for Quotation

With Cellartis directed differentiation services, we will deliver high-quality, custom hepatocytes or definitive endoderm cells derived from your human ES or iPS cells.

Notice to purchaser

*You must be logged in to a Purchasing Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

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