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Lentiviral particles for fluorescent whole-cell labeling

Fluorescent premade lentivirus for whole-cell labeling

Cells transduced with these premade, super-high titer particles will express a bright fluorescent protein in one of four colors: green (ZsGreen1), red (mCherry or tdTomato), yellow (ZsYellow1) or blue (AmCyan1). The fluorescent proteins are predominantly expressed in the cytoplasm, allowing the cells to be labeled as a control for cell-tracing and cell-tracking studies.

For fluorescent labeling of specific cellular organelles or structures, visit the fluorescent subcellular labeling lentivirus page.

Our products are available in two sizes each. 

Cat. # Product Size Price License Quantity Details
0038VCT rLV.EF1.ZsGreen1-9 1 x 20 uL USD $852.00

The Flash Therapeutics Constitutive Lentiviral Reporter Vectors are prepackaged lentiviral particles expressing fluorescent protein genes for transducing mammalian cells. The expression of fluorescent protein genes is driven by an EF1α promoter. These VSV-G pseudotyped viruses are capable of infecting both dividing and non-dividing cells. The high purification level of these lentiviral particles allows transduction of primary cells and immortalized cell lines without inducing any toxicity to the cells, even at high multiplicity of infection (M.O.I.).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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0037VCT rLV.EF1.mCherry-9 1 x 20 uL Inquire for Quotation *

The Flash Therapeutics Constitutive Lentiviral Reporter Vectors are prepackaged lentiviral particles expressing fluorescent protein genes for transducing mammalian cells. The expression of fluorescent protein genes is driven by an EF1α promoter. These VSV-G pseudotyped viruses are capable of infecting both dividing and non-dividing cells. The high purification level of these lentiviral particles allows transduction of primary cells and immortalized cell lines without inducing any toxicity to the cells, even at high multiplicity of infection (M.O.I.).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data
0036VCT rLV.EF1.tdTomato-9 1 x 20 uL Inquire for Quotation *

The Flash Therapeutics Constitutive Lentiviral Reporter Vectors are prepackaged lentiviral particles expressing fluorescent protein genes for transducing mammalian cells. The expression of fluorescent protein genes is driven by an EF1α promoter. These VSV-G pseudotyped viruses are capable of infecting both dividing and non-dividing cells. The high purification level of these lentiviral particles allows transduction of primary cells and immortalized cell lines without inducing any toxicity to the cells, even at high multiplicity of infection (M.O.I.).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data
0039VCT rLV.EF1.AmCyan1-9 1 x 20 uL USD $856.00

The Flash Therapeutics Constitutive Lentiviral Reporter Vectors are prepackaged lentiviral particles expressing fluorescent protein genes for transducing mammalian cells. The expression of fluorescent protein genes is driven by an EF1α promoter. These VSV-G pseudotyped viruses are capable of infecting both dividing and non-dividing cells. The high purification level of these lentiviral particles allows transduction of primary cells and immortalized cell lines without inducing any toxicity to the cells, even at high multiplicity of infection (M.O.I.).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data
0040VCT rLV.EF1.ZsYellow1-9 1 x 20 uL USD $856.00

The Flash Therapeutics Constitutive Lentiviral Reporter Vectors are prepackaged lentiviral particles expressing fluorescent protein genes for transducing mammalian cells. The expression of fluorescent protein genes is driven by an EF1α promoter. These VSV-G pseudotyped viruses are capable of infecting both dividing and non-dividing cells. The high purification level of these lentiviral particles allows transduction of primary cells and immortalized cell lines without inducing any toxicity to the cells, even at high multiplicity of infection (M.O.I.).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

*You must be logged in to a Purchasing Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

Tips and FAQs Lentiviral particle FAQs
Overviews Premade lentiviral particles
Webinars Premade lentivirus webinar

Overview

  • What does 109 TU/ml mean?
    • In each microliter, there will be at least 1 x 106 functional lentivirus particles.
    • Each 60-µl kit is sufficient for 30 transductions (using an MOI of 10) in a 6-well plate containing 2 x 105 cells/well.
  • Premium quality—produced using a multi-step purification process that results in:
    • Very high titer; functional titer of >1 x 109 transduction units per ml (TU/ml).
    • High purity—transduce target cells without cytotoxicity.
    • >90% transduction efficiency even at very low MOI.
  • Functional titer provided, not a just a particle count
  • tdTomato and ZsGreen1 are exceptionally bright fluorescent proteins, 6X and 2.5X brighter than EGFP, respectively.
  • These vectors have a puromycin resistance cassette and the stable clones integrating these constructs can be selected using puromycin.

More Information

Applications

  • Labeling and tracking mammalian cells using bright fluorescent proteins

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Learn more about the technology used to make our ready-to-use lentivirus particle preps

Super-pure and concentrated premade lentivirus particles

Unlike ready-to-use lentiviral particles from other suppliers, the particles in our catalog are produced via a multistep purification process that results in very high titers and high purity. High titers increase transduction efficiency, while high purity reduces cytotoxicity, allowing you to successfully transduce a large number of different cell types—including primary, immortalized, adherent, and suspension cells—while retaining high cell viability.

Technology overview Lentiviral particles FAQs

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