PrimeSTAR GXL Premix Fast, Dye plus

PrimeSTAR GXL Premix Fast, Dye plus is a convenient 2X PCR master mix containing PrimeSTAR GXL DNA Polymerase, a blue gel-loading dye, and a density reagent for easy processing. PrimeSTAR GXL polymerase is a hot-start, high-fidelity enzyme optimized to amplify GC-rich templates, samples with excess templates, and long PCR products up to 30 kb.

PrimeSTAR GXL Premix Fast, Dye plus enables you to save time and simplify your amplification workflow by incorporating unsurpassed processivity and sensitivity. Simply add primers and template, and run the PCR. Additionally, you can rapidly screen your amplicons by loading them onto an agarose gel immediately after amplification or digesting directly with restriction enzymes, without purification.

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Cat. #	Product	Size	Price	License	Quantity	Details
R052A	PrimeSTAR® GXL Premix Fast, Dye plus	200 Rxns	USD $460.00
This optimized, 2X master mix comprises PrimeSTAR GXL DNA Polymerase (a high-fidelity DNA polymerase), reaction buffer, dNTP mixture, blue gel-loading dye, and density reagent. PrimeSTAR GXL polymerase is a robust enzyme for use in difficult reactions, including amplification of GC-rich regions (without requiring special reaction conditions), in the presence of excess template, and with long targets up to 30 kb in length. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back R052A: PrimeSTAR GXL Premix Fast, Dye plus

Overview

Amplification of products up to 30 kb (human genomic DNA), 40 kb (lambda DNA), or 13.5 kb (human cDNA)
Fast PCR extension (5 sec/kb)
Antibody-mediated hot-start formulation
High tolerance for GC-rich templates as well as the presence of excess nucleic acid templates

More Information

Applications:

Cloning (available in combination with In-Fusion reagent)
Sequencing/Library Prep
Multiplex HLA typing
Any difficult or variable samples
Antibody engineering
Reverse genetics system for SARS-CoV-2 using CPER method

Termini of PCR products

PrimeSTAR GXL DNA Polymerase generates products with blunt ends. It is suitable for use in conjunction with blunt-ended cloning systems.

Recommended maximum amplicon length

Up to 30 kb (human genomic DNA template)
Up to 40 kb (lambda DNA template)
Up to 13.5 kb (human cDNA template)

Bulk, custom, and OEM information

If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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