ThruPLEX FLEX: representative data from difficult samples
Note: The protocols and QC procedures for ThruPLEX HV kits have been updated to accommodate lower inputs and compatibility with the Unique Dual Index Kit sets. While product naming has been revised accordingly (ThruPLEX FLEX), reagent formulations remain unchanged.
- Streamlined workflow with high reproducibility
- Robust performance with FFPE and cfDNA
- Accurate detection of rare mutations with molecular tags
- Coverage for a broad range of GC content with microbiome samples
- Unique dual indexes for use on the NovaSeq™ system
Simple workflow: one tube and done
Addition-only steps provide a faster, failproof library prep experience without lengthy bead purifications that cause sample loss and impact data quality. Without hidden steps—like adapter dilution—it is easy to automate, scale, and standardize. Pre-plated, single-use UDI indexes make sample assignment clear for your precious materials. Learn more »
Figure 1. ThruPLEX FLEX chemistry features a fast, simple workflow without bead purification after ligation—saving time, money, and resources
Reproducible high-quality data from FFPE
Formalin-fixed paraffin-embedded samples are a gold mine of data for understanding disease—if you are equipped to tackle these challenging samples. ThruPLEX FLEX kits have been optimized to produce high-quality, reproducible data from FFPE tissue. View data »
Figure 2. Expect highly reproducible and consistent performance at a broad range of inputs, even for highly degraded FFPE DNA.
Reliably detect low-frequency mutations from cfDNA with molecular tags
Cell-free samples, such as plasma, pose significant challenges to scientists interested in using minimally invasive techniques to look at DNA—especially when the biomarkers of interest are rare. ThruPLEX Tag-Seq FLEX provides an elegant solution, with chemistry designed to generate complex libraries and molecular tags that enable accurate detection of rare mutations. View data »
| 50-ng input | 10-ng input | ||||||||||||||
| Gene | Nucleotide | Amino acid | Cosmic ID | WT | WT | 5% | 5% | 1% | 1% | WT | WT | 5% | 5% | 1% | 1% |
| EGFR | 2573T>G | L858R | COSM6224 | 0.0% | 0.0% | 6.5% | 5.2% | 1.3% | 1.5% | 0.0% | 0.0% | 5.7% | 5.8% | 1.3% | 2.1% |
| EGFR | 2369C>T | T790M | COSM6240 | 0.0% | 0.0% | 2.6% | 5.6% | 0.4% | 1.3% | 0.0% | 0.0% | 2.4% | 1.9% | 1.5% | 1.9% |
| KRAS | 35G>A | G12D | COSM521 | 0.0% | 0.0% | 4.0% | 3.8% | 1.3% | 1.6% | 0.0% | 0.0% | 3.5% | 3.6% | 1.5% | 0.5% |
| NRAS | 181C>A | Q61K | COSM580 | 0.0% | 0.0% | 8.6% | 5.1% | 2.4% | 1.7% | 0.0% | 0.0% | 6.4% | 5.1% | 2.5% | 2.3% |
| PIK3CA | 1633G>A | E545K | COSM763 | 0.0% | 0.0% | 4.2% | 3.8% | 0.9% | 1.3% | 0.0% | 0.0% | 4.2% | 5.7% | miss | 1.5% |
Figure 3. Accurately detect low-frequency mutations with the ThruPLEX Tag-Seq FLEX system, featuring discrete, balanced molecular tags.
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