TB Green Premix Ex Taq II (Tli RNase H Plus) is a TB Green Master Mix that effectively suppresses amplification of primer dimers and off-target products, and is therefore recommended for real-time PCR (qPCR) when high specificity is critical. Use this product when primer design has been optimized, but when an increase in specificity of the qPCR assay is still needed. TB Green Premix Ex Taq II (Tli RNase H Plus) includes TB Green, a reagent designed for intercalator-based qPCR.
TB Green Premix Ex Taq II (Tli RNase H Plus) is a TB Green Master Mix that effectively suppresses amplification of primer dimers and off-target products, and is therefore recommended for real-time PCR (qPCR) when high specificity is critical. Use this product when primer design has been optimized, but when an increase in specificity of the qPCR assay is still needed. TB Green Premix Ex Taq II (Tli RNase H Plus) includes TB Green, a reagent designed for intercalator-based qPCR. Additionally, TB Green Premix Ex Taq II (Tli RNase H Plus) is supplied at a 2X concentration, enabling easy reaction assembly. The premix also contains heat-resistant Tli RNase H Plus, which effectively degrades residual mRNA found in the input cDNA, thereby minimizing qPCR inhibition as a result of residual mRNA presence.
Our TB Green Master Mix products contain TaKaRa Ex Taq DNA Polymerase Hot-Start Version, a hot-start PCR enzyme that includes an anti-Taq antibody. The use of an anti-Taq antibody allows highly reproducible and reliable qPCR. TB Green Premix ExTaq II (Tli RNase H Plus) also enables high reaction specificity and superior performance with GC-rich templates.
High specificity minimizes primer-dimer formation and nonspecific amplification
Excels with GC-rich targets
2X master mix provides convenience
High sensitivity: detects fewer than 100 copies
Compatible with all commonly used qPCR instruments
Includes TB Green Master Mix for intercalator-based qPCR
Tli RNase H Plus reduces inhibition from mRNA/cDNA hybrids in qPCR
Eliminates the need for a separate RNase H digestion step when using low RNase H RT
More Information
Applications
Gene expression analysis
qPCR
Genotyping
Suppression of primer dimers or off-target products
Components
Two ROX reference dyes are supplied as separate components with Cat. # RR820A, RR820B, RR820L, and RR820W. These reference dyes serve as convenient internal reference standards when normalizing non-PCR generated fluorescence fluctuations; such fluctuations often occur between different wells or over time in different instruments.
Cat. # RR820L and RR820W are larger package sizes (5 ml and 25 ml (5 x 5 ml), respectively) of TB Green Premix Ex Taq II (Tli RNase H Plus). Each of these includes a separate vial of ROX Reference Dye.
Cat. # RR82LR and RR82WR are larger package sizes (5 ml and 25 ml (5 x 5 ml), respectively) with ROX Reference Dye included in the premix.
Product citations
Hong, S. et al. Type II protein arginine methyltransferase 5 (PRMT5) is required for circadian period determination in Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA107:21211–21216 (2010).
Ishikawa, M. et al. Downregulation of glutamine synthetase via GLAST suppression induces retinal axonal swelling in a rat ex vivo hydrostatic pressure model. Invest. Ophthalmol. Vis. Sci.52:6604–6616 (2011).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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