Lentivirus titration by p24 ELISA

The Lenti-X p24 Rapid Titer Kit (Single Wash) and the Lenti-X p24 Rapid Titer Kit are designed for the rapid determination of lentiviral supernatant titers by p24 ELISA. (See the workflow comparison for both the kits.)

Why do you need to know your titer?

Confirm the success of your packaging reaction to avoid wasting time with your experiments
Perform consistent experiments
Ensure sufficient MOI so that 100% of your cells are transduced
Control copy number and hence expression levels

The Lenti-X p24 Rapid Titer Kit (Single Wash) and the Lenti-X p24 Rapid Titer Kit are designed for the rapid determination of lentiviral supernatant titers by p24 ELISA.

Why do you need to know your titer?

Confirm the success of your packaging reaction to avoid wasting time with your experiments
Perform consistent experiments
Ensure sufficient MOI so that 100% of your cells are transduced
Control copy number and hence expression levels

What is p24?

Recombinant lentiviruses generated for research use contain major structural proteins, including envelope proteins, matrix proteins, and virus core proteins. The gag gene encodes the viral capsid protein (p24), the nucleocapsid proteins (p6 and p7), and a matrix protein (p17) (see data images by expanding the Details tab in the product table). The Lenti-X p24 Rapid Titer Kit specifically measures the amount of p24 capsid protein present in your viral supernatant. The level of p24 correlates directly with virus titer.

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Cat. #	Product	Size	Price
631476	Lenti-X™ p24 Rapid Titer Kit (Single Wash)	96 Rxns	USD $667.00
The Lenti-X p24 Rapid Titer Kit (Single Wash) allows you to quickly determine the titer of any HIV-1-based lentiviral vector supernatant using a fast and easy ELISA-based protocol. The test samples are mixed with anti-p24 antibody, HRP-conjugated secondary antibody, and lysis buffer. This mix is then added to the wells of the included microtiter plate (12 x 8-well strips) which are coated with anti-HIV-1 p24 capture antibody and binds the p24 (an HIV-1 core protein) in your test samples. After a single wash step, the specifically-bound p24 in the sample is detected with a color-producing substrate. Color intensity is measured spectrophotometrically to indicate the level of p24 in the samples, which is then quantified against a p24 standard curve in order to titrate the viral content. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Lentiviral titration by p24 ELISA or Lenti-X GoStix Lentiviral titration by p24 ELISA or Lenti-X GoStix. The p24 protein is located in the lentiviral capsid and is one of four proteins encoded by the HIV-1 gag gene. The amount of p24 present in packaging cell supernatants can be used to measure lentiviral titer. Back The Lenti-X p24 Rapid Titer Kit (Single Wash) workflow Workflow. Lentiviral p24 core protein in packaging cell supernatants is bound to wells of a microtiter plate coated with p24 capture antibody. The presence of bound p24 in the wells is detected using an anti-p24 antibody, HRP-conjugated secondary antibody, and a color-producing substrate. Quantitation is performed by comparing test samples to a p24 standard curve. Back p24 Titer Kits comparison Schematic comparing the workflow of the Lenti-X p24 Rapid Titer Kit (Single Wash) (Cat # 631476) to Lenti-X p24 Rapid Titer Kit (Cat # 632200). The Lenti-X p24 Rapid Titer Kit (Single Wash) protocol involves fewer steps and only a single wash step. Back Lenti-X p24 Rapid Titer Kit (Single Wash) standard curve Lenti-X p24 Rapid Titer Kit (Single Wash) standard curve. p24 standard dilutions were prepared and assayed as described in the user manual. The table shows the assay results for the p24 standard dilutions and the graph was generated from the values in the table.
632200	Lenti-X™ p24 Rapid Titer Kit	96 Rxns	USD $667.00
The Lenti-X p24 Rapid Titer Kit allows you to quickly determine the titer of any HIV-1-based lentiviral vector supernatant using a standard ELISA. The wells of the included microtiter plate (12 x 8-well strips) are coated with anti-HIV-1 p24 capture antibody, which binds the p24 (an HIV-1 core protein) in your test samples. Specifically-bound p24 is detected in a typical "sandwich" ELISA format using a biotinylated anti-p24 secondary antibody, a streptavidin-HRP conjugate, and a color-producing substrate. Color intensity is measured spectrophotometrically to indicate the level of p24 in the samples, which is then quantified against a p24 standard curve in order to titrate the viral content. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Resources Back Lentiviral titration by p24 ELISA or Lenti-X GoStix Lentiviral titration by p24 ELISA or Lenti-X GoStix. The p24 protein is located in the lentiviral capsid and is one of four proteins encoded by the HIV-1 gag gene. The amount of p24 present in packaging cell supernatants can be used to measure lentiviral titer. Back Lenti-X p24 Rapid Titer Kit to measure lentivirus titer Lenti-X p24 Rapid Titer Kit to measure lentivirus titer. Lentiviral supernatant is bound to anti-p24-coated wells and detected using a combination of biotinylated anti-p24 secondary antibody, streptavidin-HRP, and a color reagent.

Overview

Lenti-X p24 Rapid Titer Kit (Single Wash)

Fast and easy-to-use p24 ELISA kit to determine the titer of your lentivirus prep (~1.5 hr)
Broader detection range enables determination of p24 titer with fewer dilution steps and iterations
Collect lentiviral supernatant, lyse, bind, wash (1X), and detect
96-well format in separable 12 x 8-well strips, offering flexibility
p24 control included

Lenti-X p24 Rapid Titer Kit

Rapidly determine the titer of your lentivirus prep using a p24 ELISA (~3 hr)
Collect lentiviral supernatant, lyse, bind, wash (3X), and detect
96-well format in separable 12 x 8-well strips offering flexibility
p24 control included

More Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Lentivirus titration kit options

We have several options available for determining the titer of your lentiviral preps. From the quick and easy Lenti-X GoStix Plus to the precise Lenti-X qRT-PCR Titration Kit, we have the solution you need to quantify lentiviral titers.

Kit options Virus product finder tool

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Lentivirus titration by p24 ELISA

Notice to purchaser

Lentiviral titration by p24 ELISA or Lenti-X GoStix

The Lenti-X p24 Rapid Titer Kit (Single Wash) workflow

p24 Titer Kits comparison

Lenti-X p24 Rapid Titer Kit (Single Wash) standard curve