Osteocalcin antibodies | Monoclonal Anti-Bovine Osteocalcin (Clone OC4-30, OCG2, OCG3 and OCG4)
Monoclonal Anti-Bovine Osteocalcin antibody (clones OC4-30,OCG2, OCG3 and OCG4) is raised against bovine osteocalcin and recognizes both bovine and human osteocalcins. Clone OC4-30 is specific for position 17 gamma-carboxylated osteocalcin and does not recognize decarboxylated osteocalcin. Osteocalcin epitopes used to generate Clones OCG2, OCG3 and OCG4 are amino acids 45–49, amino acids 21–31 and amino acids 4–9, respectively. This collection of osteocalcin antibody clones facilitates osteocalcin immunohistochemistry studies which can also be performed on paraffin-embedded tissues (as described in the Resources section of the Product Table). In addition to cross-reactivity with bovine and human osteocalcin, clones OCG4-30, OCG3 and OCG4 are also capable of recognizing osteocalcin in several other species as listed. For species-specific osteocalcin detection, Takara Bio offers additional osteocalcin antibody products.
Overview
- All clones can be used for Western blot under reducing or non-reducing conditions.
- Clones OCG4-30, OCG2 and OCG4 can be used for osteocalcin immunohistochemistry on frozen and paraffin-embedded tissue sections.
- Clone OCG3 can also be used for osteocalcin immunohistochemistry on frozen tissue sections. It has weaker reactivity on paraffin-embedded tissue sections than the other clones.
More Information
Source
| Clone | Cat. # | Immunogen | Specificity | Does not react with |
| OC4-30 | M041 | bovine osteocalcin | Reacts with bovine, human, rat, rabbit, porcine, dog, goat, sheep, chicken, mouse | — |
| OCG2 | M042 | bovine osteocalcin | Reacts with bovine, human | Does not react with mouse, rat |
| OCG3 | M043 | bovine osteocalcin | Reacts with bovine, human, rat, rabbit, porcine, goat, sheep | Does not react with mouse |
| OCG4 | M044 | bovine osteocalcin | Reacts with bovine, human, rabbit, porcine, dog, goat, sheep, chicken | Does not react with mouse, rat |
For research use only. Not for use in diagnostic or therapeutic procedures.
Form
Lyophilized. Dissolve the lyophilized antibody in 50 µl of Reconstitution Solution. (Final concentration: 2.0 mg/ml)
Product citations
Engvall, et al., Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J. Immunol. 109(1):129–135 (1972).
Hauschka, et al., Direct identification of the calcium-binding amino acid, gamma-carboxyglutamate, in mineralized tissue. Proc. Natl. Acad. Sci. U.S.A. 72(10):3925–3929 (1975).
Kayama, et al., A one step sandwich enzyme immunoassay for γ-carboxylated osteocalcin using monoclonal antibodies. J. Immunol. Methods 139(1):17–23 (1991).
Vergnaud, et al. Undercarboxylated Osteocalcin Measured with a Specific Immunoassay Predicts Hip Fracture in Elderly Women: The EPIDOS Study. J. Clin. Endocrinol. Metab., 82(3):719–724 (1997).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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