Hepatic cell differentiation antibodies: alpha-fetoprotein (AFP) and albumin detection
During hepatic cell differentiation from pluripotent cells (iPS and ES cells), parallel measurement of alpha-fetoprotein (AFP) and albumin can be used to monitor the acquisition of differentiated cellular function. AFP is normally produced by cells in the liver and yolk sac during fetal development and is thought to be important for prenatal hematopoietic function. Albumin replaces AFP function after birth, and therefore, high levels of AFP are rarely detected in normal adult blood. However, elevated levels of AFP occur with various diseases of the liver, including acute hepatitis, liver cirrhosis, and liver cancer.
During hepatic cell differentiation from pluripotent cells (iPS and ES cells), parallel measurement of alpha-fetoprotein (AFP) and albumin can be used to monitor the acquisition of differentiated cellular function. AFP is normally produced by cells in the liver and yolk sac during fetal development and is thought to be important for prenatal hematopoietic function. Albumin replaces AFP function after birth, and therefore, high levels of AFP are rarely detected in normal adult blood. However, elevated levels of AFP occur with various diseases of the liver, including acute hepatitis, liver cirrhosis, and liver cancer.
Antibodies for AFP and albumin detection
The Monoclonal Antibody to Human Alpha Fetoprotein can be used for Western blot analysis or immunocytochemical or immunohistochemical staining of human AFP. Albumin monoclonal antibodies are available for detection of either human, mouse, or rat albumin by immunocytochemical, immunohistochemical, or Western blot methods.
Overview
| Target | Cat. # | Application | Host species | Clone and source | Cross-reactivity |
| Human Alpha Fetoprotein | M225 | WB, ICC, IHC | Mouse | Clone # AFP 16 Antibody raised against purified AFP obtained from human plasma |
Cross-reacts with porcine AFP, and weakly cross-reacts with mouse AFP
|
| Human albumin | M226 | WB, ICC, IHC | Mouse | Clone # hAlb 3-7A Antibody raised against purified albumin obtained from human plasma |
Does not cross-react with bovine, porcine, rabbit, rat, mouse, or monkey albumin
|
| Mouse albumin | M234 | WB, ICC, IHC | Mouse | Clone # M-Alb 151-1 Antibody raised against purified albumin obtained from mouse plasma |
Does not cross-react with human, bovine, rat, porcine, or goat albumin |
| Rat albumin | M235 | WB, ICC, IHC | Mouse | Clone # R-Alb 214A-1 Antibody raised against purified albumin obtained from rat plasma |
Does not cross-react with human, bovine, mouse, porcine, or goat albumin |
More Information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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