Vitronectin detection

Vitronectin (VN) is a major adhesive protein in blood also known as a complement-binding protein (S-protein). VN is thought to adhere to cells with vitronectin receptors, and is involved in the immune system and blood coagulation. It helps regulate fibrinolytic system in blood vessels by binding to various blood coagulation factors (e.g., collagen, heparin, plasminogen activator inhibitor-1 (PAI-1), thrombin, antithrombin III complex, etc.). VN has a single polypeptide chain structure consisting of 459 amino acids (processed from a 478-amino acid precursor), and its molecular weight is 75 kDa. Its cell binding region has an RGD (Arg-Gly-Asp) sequence, and a sequence similar to hemopexin (a heme-binding protein in blood) is repeated at the center of the region. The C-terminus has a heparin-binding domain that is hidden under native conditions, but can be exposed by treatment with denaturing reagents or by heating. Therefore, VN can be isolated with high purity by binding to a heparin column in the presence of a denaturing agent, such as urea. In some cases, the C-terminus is cleaved between amino acids 389 and 390 due to an amino acid mutation, and the resulting 65-kDa protein is often present in blood in addition to the 75-kDa form. However, the reason for the existence of these two structures and their physiological functions are not known.

ELISAs for vitronectin detection

This product is a solid-phase sandwich ELISA kit using two antibodies specific to human and rabbit VN (both 65- and 75-kDa forms): one is precoated on the ELISA plate and the other is POD-conjugated. This kit can be used to measure soluble human or rabbit VN protein in blood, urine, and cell culture supernatant. This kit does not cross-react with bovine antigens. Therefore, cell culture supernatants collected from cells cultured with medium containing fetal bovine serum can be measured directly.

Antibodies for vitronectin detection

Human Vitronectin (VN) Antibody is a monoclonal antibody that can be used for detecting VN by Western blot (WB) or immunohistochemistry in frozen or paraffin-embedded tissue sections. This antibody does not interfere with the cell-binding activity of VN.