POD conjugates for immunohistochemical staining of paraffin-embedded tissue sections

Takara POD conjugates are designed to replace traditional secondary antibodies in immunohistochemical staining of paraffin-embedded tissue sections. Use these products for a simpler and easier method than those based on streptavidin-biotin, and obtain clear, highly sensitive results with minimal background.

Each Takara POD conjugate consists of an amino acid polymer combined with peroxidase and the Fab' fragments of a secondary antibody. The labeled polymers bind to the primary antibody-antigen to form an HRP-labeled complex. The complex is visualized by the addition of a colorimetric substrate, such as DAB Substrate (see below).

Each Takara POD conjugate consists of an amino acid polymer combined with peroxidase and the Fab' fragments of a secondary antibody. The labeled polymers bind to the primary antibody-antigen to form an HRP-labeled complex. The complex is visualized by the addition of a colorimetric substrate.

This product is available in two different formats, for mouse tissue sections ("For Mouse Tissue") and for tissue sections from other mammalian sources ("For Tissue"). Specific versions are available based on the source species of the primary antibody; "Anti Mouse", "Anti Rat", and "Anti Rabbit" versions are available for mouse tissue sections, and "Anti Mouse", "Anti Rabbit", and "Anti Goat" versions are available for mammalian tissue.

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Cat. #	Product	Size	Price
MK201	TaKaRa® POD Conjugate Anti Rat, For Mouse Tissue	6 mL	USD $159.00
TaKaRa POD Conjugate is a reagent designed for immunohistochemical staining of paraffinembedded tissue sections. This product consists of an amino acid polymer combined with peroxidase and Fab' fragments of a secondary antibody. It is supplied at a concentration suitable for immediate use, in MOPS (3-(N -Morpholino) propanesulfonic acid) buffer (pH6.5) containing stabilizing protein and antibiotics. This product provides clear, highly sensitive results with minimal background staining, and is simpler and easier to use than the streptavidin-biotin method. This product is available in two different formats, "For Mouse Tissue" (for mouse tissue sections) and "For Tissue" (for tissue sections from other mammalian sources). Three specific versions are available in each format, based on the source species of the primary antibody. "Anti Mouse", "Anti Rat" and "Anti Rabbit" versions are available "For Mouse Tissue", and "Anti Mouse", "Anti Rabbit" and "Anti Goat" versions are available "For Tissue". The TaKaRa POD Conjugate Set Anti Mouse, For Mouse Tissue (Cat. #MK200) is unique because it may be used with a mouse primary antibody on mouse tissue. It consists of Blocking Reagents A and B and TaKaRa POD Conjugate Anti Mouse, For Mouse Tissue. The "For Tissue" products can be used with tissue sections from various species, but it is important to determine in advance the appropriate conditions for each tissue and primary antibody. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components
MK202	TaKaRa® POD Conjugate Anti Rabbit, For Mouse Tissue	6 mL	USD $159.00
TaKaRa POD Conjugate is a reagent designed for immunohistochemical staining of paraffinembedded tissue sections. This product consists of an amino acid polymer combined with peroxidase and Fab' fragments of a secondary antibody. It is supplied at a concentration suitable for immediate use, in MOPS (3-(N -Morpholino) propanesulfonic acid) buffer (pH6.5) containing stabilizing protein and antibiotics. This product provides clear, highly sensitive results with minimal background staining, and is simpler and easier to use than the streptavidin-biotin method. This product is available in two different formats, "For Mouse Tissue" (for mouse tissue sections) and "For Tissue" (for tissue sections from other mammalian sources). Three specific versions are available in each format, based on the source species of the primary antibody. "Anti Mouse", "Anti Rat" and "Anti Rabbit" versions are available "For Mouse Tissue", and "Anti Mouse", "Anti Rabbit" and "Anti Goat" versions are available "For Tissue". The TaKaRa POD Conjugate Set Anti Mouse, For Mouse Tissue (Cat. #MK200) is unique because it may be used with a mouse primary antibody on mouse tissue. It consists of Blocking Reagents A and B and TaKaRa POD Conjugate Anti Mouse, For Mouse Tissue. The "For Tissue" products can be used with tissue sections from various species, but it is important to determine in advance the appropriate conditions for each tissue and primary antibody. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Immunohistochemical detection of neural progenitor cells in mouse cerebellum Immunohistochemical detection of neural progenitor cells in mouse cerebellum. A section of formalin-fixed paraffin-embedded mouse cerebellum tissue was stained with an anti-L7/Pcp2 antibody (Cat. # M194). Takara POD Conjugate Anti Rabbit, For Mouse Tissue (Cat. # MK202) was used in place of a traditional secondary antibody, and Takara DAB Substrate (Cat. # MK210) was used for detection. The image shown is 100X magnification.
MK203	TaKaRa® POD Conjugate Anti Goat, For Tissue	6 mL	USD $159.00
TaKaRa POD Conjugate is a reagent designed for immunohistochemical staining of paraffinembedded tissue sections. This product consists of an amino acid polymer combined with peroxidase and Fab' fragments of a secondary antibody. It is supplied at a concentration suitable for immediate use, in MOPS (3-(N -Morpholino) propanesulfonic acid) buffer (pH6.5) containing stabilizing protein and antibiotics. This product provides clear, highly sensitive results with minimal background staining, and is simpler and easier to use than the streptavidin-biotin method. This product is available in two different formats, "For Mouse Tissue" (for mouse tissue sections) and "For Tissue" (for tissue sections from other mammalian sources). Three specific versions are available in each format, based on the source species of the primary antibody. "Anti Mouse", "Anti Rat" and "Anti Rabbit" versions are available "For Mouse Tissue", and "Anti Mouse", "Anti Rabbit" and "Anti Goat" versions are available "For Tissue". The TaKaRa POD Conjugate Set Anti Mouse, For Mouse Tissue (Cat. #MK200) is unique because it may be used with a mouse primary antibody on mouse tissue. It consists of Blocking Reagents A and B and TaKaRa POD Conjugate Anti Mouse, For Mouse Tissue. The "For Tissue" products can be used with tissue sections from various species, but it is important to determine in advance the appropriate conditions for each tissue and primary antibody. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components
MK204	TaKaRa® POD Conjugate Anti Mouse, For Tissue	6 mL	USD $159.00
TaKaRa POD Conjugate is a reagent designed for immunohistochemical staining of paraffinembedded tissue sections. This product consists of an amino acid polymer combined with peroxidase and Fab' fragments of a secondary antibody. It is supplied at a concentration suitable for immediate use, in MOPS (3-(N -Morpholino) propanesulfonic acid) buffer (pH6.5) containing stabilizing protein and antibiotics. This product provides clear, highly sensitive results with minimal background staining, and is simpler and easier to use than the streptavidin-biotin method. This product is available in two different formats, "For Mouse Tissue" (for mouse tissue sections) and "For Tissue" (for tissue sections from other mammalian sources). Three specific versions are available in each format, based on the source species of the primary antibody. "Anti Mouse", "Anti Rat" and "Anti Rabbit" versions are available "For Mouse Tissue", and "Anti Mouse", "Anti Rabbit" and "Anti Goat" versions are available "For Tissue". The TaKaRa POD Conjugate Set Anti Mouse, For Mouse Tissue (Cat. #MK200) is unique because it may be used with a mouse primary antibody on mouse tissue. It consists of Blocking Reagents A and B and TaKaRa POD Conjugate Anti Mouse, For Mouse Tissue. The "For Tissue" products can be used with tissue sections from various species, but it is important to determine in advance the appropriate conditions for each tissue and primary antibody. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Immunohistochemical detection of Osteonectin/SPARC in miniature pig tissue Immunohistochemical detection of Osteonectin/SPARC in miniature pig tissue. A section of paraffin-embedded miniature pig joint tissue was stained with an anti-Osteonectin/SPARC monoclonal antibody (Cat. # M125). Takara POD Conjugate Anti Mouse, For Tissue (Cat. # MK204) was used in place of a traditional secondary antibody, and Takara DAB Substrate (Cat. # MK210) was used for detection. The image shown is 100X magnification.

Overview

Takara POD conjugates

Do not react with endogenous immunoglobulins (Ig)
Not affected by endogenous biotin
No need for a blocking reaction or addition of an enzyme-conjugated reagent that reacts with the secondary antibody

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Applications for Takara POD conjugates

Immunohistochemical staining of paraffin-embedded tissue sections
Antibody detection without the use of an enzyme-conjugated secondary antibody

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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POD conjugates for immunohistochemical staining of paraffin-embedded tissue sections

Notice to purchaser

Notice to purchaser

Immunohistochemical detection of neural progenitor cells in mouse cerebellum

Notice to purchaser

Notice to purchaser

Immunohistochemical detection of Osteonectin/SPARC in miniature pig tissue

Overview

Takara POD conjugates

More Information

Applications for Takara POD conjugates

Additional product information