Peptide Coating Kit

Generally, low molecular weight peptides and synthetic peptides do not spontaneously attach to 96-well microtiter ELISA plates. The Peptide Coating Kit is designed to allow optimal peptide plate coating onto 96-well plates. The Peptide Coating Kit also includes reaction buffer, 96-well microplates, and blocking solution designed specifically for subsequent ELISA.

Principle

The free carboxyl groups of peptides used for plate coating can be covalently linked via reaction with the water-soluble peptide coupling reagent l-ethyl-3-(3-(dimethylamino)-propy1)carbodiimide (EDC). These peptides can subsequently be linked to amino groups that are attached to the surface of microtiter plate wells. The end result is a population of peptides directly coating the surface of the microtiter plate via peptide bonds.

Cat. #	Product	Size	Price	License	Quantity	Details
MK100	Peptide Coating Kit	480 Rxns	USD $375.00
The Peptide Coating Kit allows you to coat plates efficiently with low molecular weight proteins or synthetic peptides that are otherwise difficult to adsorb onto plates. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back MK100: Peptide Coating Kit

More Information

Applications

Detection of antigen via ELISA
Detection of antibody via competitive ELISA

References

Nakajima, N. & Ikada, Y. Mechanism of amide formation by carbodiimide for bioconjugation in aqueous media. Bioconjug. Chem. 6, 123–130 (1995).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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