DNA Polymerase I, Klenow Fragment

DNA Polymerase I, Klenow Fragment (Large Fragment) is a proteolytic product of E. coli DNA Polymerase I, which possesses the 5'→3' polymerase and 3'→5' exonuclease activities of intact DNA Polymerase I, but lacks the 5'→3' exonuclease activity of full-length DNA Polymerase I. The lack of 5'→3' exonuclease activity enables the DNA Polymerase I, Klenow Fragment to be used in random primer labeling and DNA sequencing experiments in addition to second-strand cDNA synthesis. The enzyme is supplied in a buffer of 50 mM potassium phosphate (pH 6.5), 1 mmDTT and 50% glyerol (2140A/B) or ethylene glycol (2140AK/BK).

Cat. #	Product	Size	Price
2140BK	Klenow Fragment for Sequencing (Large Fragment E. coli DNA Polymerase I)	1,000 Units	USD $428.00
Klenow Fragment (DNA Polymerase I Large Fragment) possesses the 5'→3' polymerase and 3'→5' exonuclease activities of intact DNA Polymerase I, but lacks its 5'→3' exonuclease activity. Klenow Fragment is supplied in 50 mM potassium phosphate (pH 6.5), 1 mm DTT, and 50% glycerol (2140A/B/S) or ethylene glycol (2140AK/BK). Cat. # 2140BK contains 5 of Cat. # 2140AK. Please refer to Cat. # 2140AK for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents
2140B	Klenow Fragment (Large Fragment E. coli DNA Polymerase I)	1,000 Units	USD $312.00
Klenow Fragment (DNA Polymerase I Large Fragment) possesses the 5'→3' polymerase and 3'→5' exonuclease activities of intact DNA Polymerase I, but lacks its 5'→3' exonuclease activity. Klenow Fragment is supplied in 50 mM potassium phosphate (pH 6.5), 1 mm DTT, and 50% glycerol (2140A/B/S) or ethylene glycol (2140AK/BK). Cat. # 2140B contains 5 of Cat. # 2140A. Please refer to Cat. # 2140A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents
2140AK	Klenow Fragment for Sequencing (Large Fragment E. coli DNA Polymerase I)	200 Units	USD $107.00
Klenow Fragment (DNA Polymerase I Large Fragment) possesses the 5'→3' polymerase and 3'→5' exonuclease activities of intact DNA Polymerase I, but lacks its 5'→3' exonuclease activity. Klenow Fragment is supplied in 50 mM potassium phosphate (pH 6.5), 1 mm DTT, and 50% glycerol (2140A/B/S) or ethylene glycol (2140AK/BK). Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 2140AK: Klenow Fragment (Large Fragment E. coli DNA Polymerase I)
2140A	Klenow Fragment (Large Fragment E. coli DNA Polymerase I)	200 Units	USD $80.00
Klenow Fragment (DNA Polymerase I Large Fragment) possesses the 5'→3' polymerase and 3'→5' exonuclease activities of intact DNA Polymerase I, but lacks its 5'→3' exonuclease activity. Klenow Fragment is supplied in 50 mM potassium phosphate (pH 6.5), 1 mm DTT, and 50% glycerol (2140A/B/S) or ethylene glycol (2140AK/BK). Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 2140A: Klenow Fragment (Large Fragment E. coli DNA Polymerase I)

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Applications

Random primer labeling
Second-strand cDNA synthesis
Dideoxy DNA sequencing
2140AK/BK are sequencing grade reagents

Buffer

Supplied with 10X Reaction Buffer [100 mM Tris-HCl (pH 7.5), 70 mM MgCl₂ and 1 mM DTT].

Concentration

2140A/B: 2–5 U/µl

2140AK/BK: 2 U/µl

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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DNA Polymerase I, Klenow Fragment

Notice to purchaser

Notice to purchaser

Notice to purchaser

2140AK: Klenow Fragment (Large Fragment E. coli DNA Polymerase I)

Notice to purchaser

2140A: Klenow Fragment (Large Fragment E. coli DNA Polymerase I)

More Information

Applications

Buffer

Concentration

Additional product information