Ribonuclease H (RNase H)

Ribonuclease H (RNase H) is an RNA-specific endonuclease that hydrolyzes single-stranded RNA via a magnesium or manganese cofactor. As such, RNase H is often used to remove the RNA template following reverse transcription or cDNA generation. Ribonuclease H (RNase H) is supplied in a buffer of 25 mM Tris-HCl (pH 7.5), 30 mM NaCl, 0.5 mM EDTA, 1 mM DTT and 50% glycerol.

Cat. #	Product	Size	Price
2150B	Ribonuclease H	5,000 Units	USD $452.00
Ribonuclease H is an endoribonuclease that specifically degrades the RNA strand in an RNA-DNA hybrid. Ribonuclease H is supplied in 25 mM Tris-HCl (pH 7.5), 30 mM NaCl, 0.5 mM EDTA, 1 mM DTT and 50% glycerol. Cat. # 2150B contains 5 of Cat. # 2150A. Please refer to Cat. # 2150A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 2150B: Ribonuclease H
2150A	Ribonuclease H	1,000 Units	USD $116.00
Ribonuclease H is an endoribonuclease that specifically degrades the RNA strand in an RNA-DNA hybrid. Ribonuclease H is supplied in 25 mM Tris-HCl (pH 7.5), 30 mM NaCl, 0.5 mM EDTA, 1 mM DTT and 50% glycerol. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 2150A: Ribonuclease H

More Information

Applications

Removal of RNA template following second-strand cDNA synthesis
Removal of mRNA poly(A) sequence
Reverse transcription

Source

Recombinant E. coli

Storage

–20°C

Unit definition

One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble product in 20 minutes at 30°C and pH 7.7 using poly(rA)-poly(dT) as a substrate.

Concentration

20–60 U/µl

Product citations

Schultz, S. J. & Champoux, J. J. RNase H activity: structure, specificity, and function in reverse transcription. Virus Res. 134, 86–103 (2008).

Vournakis, J. N., Efstratiadis, A. & Kafatos, F. C. Electrophoretic patterns of deadenylylated chorion and globin mRNAs. Proc. Natl. Acad. Sci. U. S. A. 72, 2959–63 (1975).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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