NucleoGone Endonuclease

NucleoGone Endonuclease is genetically engineered to efficiently degrade a variety of DNA and RNA types, including single-stranded, double-stranded, linear, and circular forms, under a wide range of reaction conditions. It can also be used to remove existing nucleic acids from host cells during protein and viral particle purification.

Cat. #	Product	Size	Price	License	Quantity	Details
2490A	NucleoGone™ Endonuclease	25,000 Units	USD $275.00
NucleoGone Endonuclease is a genetically engineered endonuclease that efficiently degrades a variety of DNA and RNA types. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back NucleoGone Endonuclease activity compared to Company A's endonuclease NucleoGone Endonuclease activity compared to Company A's endonuclease. NucleoGone Endonuclease and Company A's endonuclease were used to digest various DNA or RNA species. For each reaction, 1 μg of DNA or RNA types was treated with 1 U of enzyme at 37°C for 30 min. NucleoGone Endonuclease performed similarly to Company A's endonuclease. Lane M1 contains the Wide-Range DNA Ladder (100-5,000 bp) (Cat. # 3427A/B), which consists of the following DNA fragments: 100, 250, 500, 750, 1,000, 1,500, 2,000, 3,000, and 5,000 bp. Lane M2 contains the Lambda (λ)-Hind III Digest (Cat. #3403), which consists of the following DNA fragments: 125, 564, 2,027, 2,322, 4,361, 6,557, 9,416, and 23,130 bp.

Overview

Degrade DNA and RNA (single-stranded, double-stranded, linear, and circular forms) across a wide range of conditions
Reduce viscosity of cell lysates during protein purification
Remove genomic DNA derived from host cells during virus purification, such as in AAV production

More Information

Applications

Protein purification
Viral particle purificaiton

Source

Escherichia coli carrying the plasmid containing the gene for NucleoGone Endonuclease.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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NucleoGone Endonuclease

Notice to purchaser