Shrimp alkaline phosphatase (SAP)

Shrimp alkaline phosphatase (SAP) nonspecifically catalyzes the dephosphorylation of 5'-termini DNA and RNA phosphomonoesters, but does not degrade diphosphate or triphosphate linkages. The enzyme acts on 5' protruding, recessive, and blunt 5'-termini. Unlike Calf intestinal alkaline phosphatase, SAP is irreversibly heat inactivated at 65°C for 15 minutes, allowing for streamlining of cloning experiments.

Cat. #	Product	Size	Price
2660B	Alkaline Phosphatase (Shrimp)	1,500 Units	USD $862.00
Shrimp Alkaline Phosphatase (SAP) nonspecifically catalyzes the dephosphorylation of most phosphate monoesters, but it does not degrade diphosphate or triphosphate linkages. It is irreversibly inactivated by heat denaturation at 65°C for 15 minutes. Cat. # 2660B contains 5 of Cat. # 2660A. Please refer to Cat. # 2660A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents
2660A	Alkaline Phosphatase (Shrimp)	300 Units	USD $218.00
Shrimp Alkaline Phosphatase (SAP) nonspecifically catalyzes the dephosphorylation of most phosphate monoesters, but it does not degrade diphosphate or triphosphate linkages. It is irreversibly inactivated by heat denaturation at 65°C for 15 minutes. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 2660A: Alkaline Phosphatase (Shrimp)

More Information

Applications

Dephosphorylation of DNA 5'-termini
Reduction of vector re-ligation and background

Product citations

Olsen, R. L., Overbø, K. & Myrnes, B. Alkaline phophatase from the hepatopancreas of shrimp (Pandalus borealis): A dimeric enzyme with catalytically active subunits. Comp. Biochem. Physiol. Part B Comp. Biochem. 99, 755–761 (1991).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2023 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.