Micrococcal Nuclease

Micrococcal Nuclease is an endonuclease that preferentially digests single-stranded DNA or RNA, especially at AT- or AU-rich regions. The enzyme will also digest double-stranded DNA or RNA, making it an essential component of chromatin immunoprecipitation (ChIP) assays. Micrococcal Nuclease digests 5'-phosphodiester bonds of DNA and RNA, yielding 3'-phosphate mononucleotides and oligonucleotides. This enzyme requires Ca²⁺ as a cofactor for its activity and is completely inactivitated by EDTA or EGTA.

Cat. #	Product	Size	Price	License	Quantity	Details
2910A	Micrococcal Nuclease	15,000 Units	USD $211.00
Micrococcal Nuclease is an endonuclease that preferentially digests single-stranded DNA and RNA, especially at AT or AU rich regions, but will also digest double-stranded DNA or RNA. This enzyme digests 5'-phosphodiester bonds of DNA and RNA, and yields 3'-phosphate mononucleotides and oligonucleotides. It is commonly used for preparation of nucleosomes during epigenetic analysis studies. This enzyme requires Ca²⁺ and is completely inactivated by EDTA or EGTA. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 2910A: Micrococcal Nuclease

More Information

Applications

Digestion of nucleotides included in crude cell extract
Digestion of chromatin for nucleosome preparation
Chromatin immunoprecipitation (ChIP)

Supplied buffer

10X Micrococcal Nuclease [200 mM Tris-HCl (pH 8.0), 50 mM NaCl, 25 mM CaCl₂]

Form

50 mM Tris-HCl (pH 8.0), 10 mM 2-mercaptoethanol, 50 mM NaCl, 50% glycerol

Product citations

Lefevre, P. & Bonifer, C. Analyzing Histone Modification Using Crosslinked Chromatin Treated With Micrococcal Nuclease. Methods Mol. Biol. 325:315–325 (2006).

Heins, J.N. et al. Procedures Nucl. Acids Res. 1:79 (1966).

Pelham, H.R. & Jackson, R.J. An Efficient mRNA-Dependent Translation System from Reticulocyte Lysates. Eur. J. Biochem. 67:247–256 (1976).

Telford, D.J. & Stewart, B.W. Micrococcal nuclease: Its specificity and use for chromatin analysis. Int. J. Biochem. 21:127–137 (1989).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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