632515
pIRES2-AcGFP1-Nuc Vector
20 ug
USD $589.00
pIRES2-AcGFP1-Nuc encodes AcGFP1 with three identical copies of the nuclear localization signal (NLS) of the simian virus 40 large T antigen fused in tandem to its C-terminus. The gene encoding the AcGFP1-Nuc fusion protein is located downstream of the IRES2 sequence. This permits both the gene of interest (cloned into the MCS upstream of the IRES2 sequence) and the AcGFP1-Nuc gene to be translated from a single bicistronic mRNA. AcGFP-1-Nuc localizes to the nucleus of a transfected cell, offering an advantage over random localization in the cytosol. It is most useful for those who are interested in monitoring translocation events in and out of the nucleus.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632511
pAcGFP1-F Vector
20 ug
USD $589.00
pAcGFP1-F encodes a fusion protein consisting of the Aequorea coerulescens green fluorescent protein and the farnesylation signal of c-Ha-ras. AcGFP1 is a variant of the Aequorea coerulescens green fluorescent protein (AcGFP). The AcGFP1 coding sequence in this construct has been optimized for efficient expression and enhanced brightness in mammalian cells. pAcGFP1-F can be used for labeling the inner leaflet of the plasma membrane.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632510
pAcGFP1-F Hyg Vector
20 ug
USD $589.00
pAcGFP1-F encodes a fusion protein consisting of the Aequorea coerulescens green fluorescent protein and the farnesylation signal of c-Ha-ras. AcGFP1 is a variant of the Aequorea coerulescens green fluorescent protein (AcGFP). The AcGFP1 coding sequence in this construct has been optimized for efficient expression and enhanced brightness in mammalian cells. pAcGFP1-F Hyg can be used for labeling the inner leaflet of the plasma membrane. The hygromycin resistance cassette allows you to create double-stable cell lines if this vector is used in conjunction with any other Living Colors vector that contains a neomycin resistance cassette.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632509
pAcGFP1-Mem Hyg Vector
20 ug
USD $589.00
pAcGFP1-Mem Hyg encodes a fusion protein consisting of the Aequorea coerulescens green fluorescent protein and the N-terminal membrane-targeting signal of neuromodulin (also called GAP-43). AcGFP1 is a variant of the Aequorea coerulescens green fluorescent protein (AcGFP) that has been optimized for brighter fluorescence and higher expression in mammalian cells. pAcGFP1-Mem Hyg can be used for labeling the plasma membrane. The hygromycin resistance cassette allows you to create double-stable cell lines if this vector is used in conjunction with any other Living Colors vector that contains a neomycin resistance cassette.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632506
pRetroQ-AcGFP1-C1 Vector
20 ug
USD $568.00
The pRetroQ-AcGFP1-C1 vector is a high-titer, self-inactivating retroviral expression vector that is optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. This vector expresses a green fluorescent protein (GFP) from Aequorea coerulescens. pRetroQ-AcGFP1-C1 allows cloning of genes into the multiple cloning site (MCS) downstream of the AcGFP1 coding sequence, which are subsequently expressed as fusions to the C-terminus of the AcGFP1 protein. This vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable integrants. The unmodified vector will express AcGFP1 protein and may be used to produce marker virus to optimize infection protocols.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632505
pRetroQ-AcGFP1-N1 Vector
20 ug
USD $568.00
The pRetroQ-AcGFP1-N1 vector is a high-titer, self-inactivating retroviral expression vector that is optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. This vector expresses a green fluorescent protein (GFP) from Aequorea coerulescens . pRetroQ-AcGFP1-N1 allows cloning of genes into the multiple cloning site (MCS) upstream of the AcGFP1 coding sequence, which are subsequently expressed as fusions to the N-terminus of the AcGFP1 protein. This vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable integrants. The unmodified vector will express AcGFP1 protein and may be used to produce marker virus to optimize infection protocols.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632501
pAcGFP1-N In-Fusion® Ready Vector
1 ug
USD $406.00
pAcGFP1-N In-Fusion Ready Vector encodes a green fluorescent protein from Aequorea coerulescens . The vector is prelinearized to enable directional In-Fusion cloning of PCR fragments onto the 5' end of the AcGFP1-encoding gene without further restriction digests. Sequences complementary to the vector sequences (flanking the site of linearization) need to be incorporated at the 5' ends of the PCR primers (see PT3865-2). The gene of interest is expressed as an N-terminal fusion to AcGFP1 in mammalian cells. The fluorescent protein coding sequence in this construct has been human codon-optimized for efficient expression and enhanced brightness in mammalian cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632500
pAcGFP1-C In-Fusion® Ready Vector
1 ug
USD $406.00
pAcGFP1-C In-Fusion Ready Vector encodes a green fluorescent protein from Aequorea coerulescens . The vector is prelinearized to enable directional In-Fusion cloning of PCR fragments onto the 3' end of the AcGFP1-encoding gene without further restriction digests. Sequences complementary to the vector sequences (flanking the site of linearization) need to be incorporated at the 5' ends of the PCR primers (see PT3865-2). The gene of interest is expressed as a C-terminal fusion to AcGFP1 in mammalian cells. The fluorescent protein coding sequence in this construct has been human codon-optimized for efficient expression and enhanced brightness in mammalian cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632497
pAcGFP1-1 Vector
20 ug
USD $589.00
pAcGFP1-1 encodes a green fluorescent protein (AcGFP1) from Aequorea coerulescens . The fluorescent protein coding sequence in this construct has been human-codon-optimized for efficient expression and enhanced brightness in mammalian cells. The vector contains a neomycin resistance gene for the selection of stable transfectants. pAcGFP1-1 can be used as a reporter to monitor the activity of promoters and promoter/enhancer combinations cloned into the multiple cloning site (MCS) upstream of the promoterless AcGFP1 gene. Without the addition of a functional promoter, this vector will not express AcGFP1.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632492
pAcGFP1-Hyg-C1 Vector
20 ug
USD $589.00
pAcGFP1-Hyg-C1 encodes a green fluorescent protein (AcGFP1) from Aequorea coerulescens . pAcGFP1-Hyg-C1 is designed for studies in mammalian systems. This vector allows expression of a protein of interest as a C-terminal fusion to AcGFP1. The vector contains a hygromycin resistance gene for the selection of stable transfectants. pAcGFP1-Hyg-C1 can also be used as a cotransfection marker since the unmodified vector constitutively expresses AcGFP1. The fluorescent protein coding sequence in this construct has been human codon-optimized for efficient expression and enhanced brightness in mammalian cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632491
pAcGFP1-Mem Vector
20 ug
USD $589.00
pAcGFP1-Mem encodes a fusion protein consisting of the Aequorea coerulescens green fluorescent protein and the N-terminal membrane-targeting signal of neuromodulin (also called GAP-43). AcGFP1 is a variant of the Aequorea coerulescens green fluorescent protein (AcGFP) that has been optimized for brighter fluorescence and higher expression in mammalian cells. pAcGFP1-Mem can be used for labeling the plasma membrane.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632490
pAcGFP1-Endo Vector
20 ug
USD $589.00
pAcGFP1-Endo encodes a fusion protein consisting of the Aequorea coerulescens green fluorescent protein, c-Myc, and RhoB protein. The RhoB GTPase localizes the fusion protein to vesicles involved in endocytosis and allows monitoring of intracellular membrane traffic in living and fixed cells using fluorescence microscopy. The c-Myc epitope allows detection of the fusion protein using c-Myc antibodies. AcGFP1 is a variant of the Aequorea coerulescens green fluorescent protein (AcGFP) that has been optimized for brighter fluorescence and higher expression in mammalian cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632489
pAcGFP1-Hyg-N1 Vector
20 ug
USD $589.00
pAcGFP1-Hyg-N1 encodes a green fluorescent protein from Aequorea coerulescens. pAcGFP1-Hyg-N1 is designed for studies in mammalian systems. This vector allows expression of a protein of interest as an N-terminal fusion to AcGFP1. The vector contains a hygromycin resistance gene for the selection of stable transfectants. The fusion vector can also be used as a cotransfection marker since the unmodified vector constitutively expresses fluorescent protein. The fluorescent protein coding sequence in this construct has been human codon-optimized for efficient expression and enhanced brightness in mammalian cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632488
pAcGFP1-Tubulin Vector
20 ug
USD $589.00
pAcGFP1-Tubulin encodes a fusion protein consisting of the AcGFP1 green fluorescent protein (GFP) and human a-tubulin. AcGFP1 is an Aequorea coerulescens GFP that has been optimized for brighter fluorescence and higher expression in mammalian cells. The AcGFP1-Tubulin fusion protein incorporates into growing microtubules, allowing visualization of the microtubule network in living or fixed cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632485
pAcGFP1-N Vector Set
Each
USD $1028.00
A set of three vectors encoding a green fluorescent protein from Aequorea coerulescens . The pAcGFP1-N vectors are designed for studies in mammalian systems. These vectors allow expression of a protein of interest as a N-terminal fusion. Each vector contains the MCS in a different reading frame with respect to AcGFP1. These fusion vectors can also be used as cotransfection markers since the unmodified vectors will express fluorescent protein. All of the fluorescent protein coding sequences in these constructs have been human-codon-optimized for efficient expression in mammalian cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632484
pAcGFP1-N3 Vector
20 ug
USD $589.00
pAcGFP1-N3 encodes a green fluorescent protein from Aequorea coerulescens . pAcGFP1-N3 is designed for studies in mammalian systems. This vector allows expression of a protein of interest as an N-terminal fusion to AcGFP1. The fusion vector can also be used as a cotransfection marker since the unmodified vector will express fluorescent protein. The fluorescent protein coding sequence in this construct has been human-codon-optimized for efficient expression and enhanced brightness in mammalian cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632483
pAcGFP1-N2 Vector
20 ug
USD $589.00
pAcGFP1-N2 encodes a green fluorescent protein from Aequorea coerulescens . pAcGFP1-N2 is designed for studies in mammalian systems. This vector allows expression of a protein of interest as an N-terminal fusion to AcGFP1. The fusion vector can also be used as a cotransfection marker since the unmodified vector will express fluorescent protein. The fluorescent protein coding sequence in this construct has been human-codon-optimized for efficient expression and enhanced brightness in mammalian cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632482
pAcGFP1-C3 Vector
20 ug
USD $589.00
pAcGFP1-C3 encodes a green fluorescent protein from Aequorea coerulescens . pAcGFP1-C3 is designed for studies in mammalian systems. This vector allows expression of a protein of interest as a C-terminal fusion to AcGFP1. The fusion vector can also be used as a cotransfection marker since the unmodified vector will express fluorescent protein. The fluorescent protein coding sequence in this construct has been human-codon-optimized for efficient expression and enhanced brightness in mammalian cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632481
pAcGFP1-C2 Vector
20 ug
USD $589.00
pAcGFP1-C2 encodes a green fluorescent protein from Aequorea coerulescens . pAcGFP1-C2 is designed for studies in mammalian systems. This vector allows expression of a protein of interest as a C-terminal fusion to AcGFP1. The fusion vector can also be used as a cotransfection marker since the unmodified vector will express fluorescent protein. The fluorescent protein coding sequence in this construct has been human-codon-optimized for efficient expression and enhanced brightness in mammalian cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632470
pAcGFP1-C1 Vector
20 ug
USD $589.00
pAcGFP1-C1 encodes a green fluorescent protein from Aequorea coerulescens. pAcGFP1-C1 is designed for studies in mammalian systems. This vector allows expression of a protein of interest as a C-terminal fusion to AcGFP1. The fusion vector can also be used as a cotransfection marker since the unmodified vector will express fluorescent protein. The fluorescent protein coding sequence in this construct has been human-codon-optimized for efficient expression and enhanced brightness in mammalian cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632469
pAcGFP1-N1 Vector
20 ug
USD $589.00
pAcGFP1-N1 encodes a green fluorescent protein from Aequorea coerulescens. pAcGFP1-N1 is designed for studies in mammalian systems. This vector allows expression of a protein of interest as an N-terminal fusion to AcGFP1. The fusion vector can also be used as a cotransfection marker since the unmodified vector will express fluorescent protein. The fluorescent protein coding sequence in this construct has been human-codon-optimized for efficient expression and enhanced brightness in mammalian cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632468
pAcGFP1 Vector
20 ug
USD $589.00
pAcGFP1 encodes a monomeric green fluorescent protein from Aequorea coerulescens . pAcGFP1 is a prokaryotic expression vector that also serves as a convenient source of fluorescent protein cDNA. In this vector, the full-length human-codon-optimized cDNA is flanked by distinct MCSs to facilitate excision from the vector and insertion into other eukaryotic or prokaryotic expression constructs. The fluorescent protein coding sequence in this construct has been human-codon-optimized for efficient expression and enhanced brightness in mammalian cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632464
pAcGFP1-Golgi Vector
20 ug
USD $589.00
The pAcGFP1-Golgi Vector encodes a fusion protein consisting of Aequorea coerulescens green fluorescent protein (AcGFP1) fused at its N-terminus to 81 amino acids of the precursor to the human beta 1,4-galactosyltransferase (GT). AcGFP1 protein is optimized for brighter fluorescence and higher expression in mammalian cells. pAcGFP1-Golgi can be used for specific labeling of the trans-medial region of the Golgi apparatus in mammalian cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632453
pAcGFP1-Actin Vector
20 ug
USD $589.00
pAcGFP1-Actin encodes a fusion protein consisting of Aequorea coerulescens green fluorescent protein (AcGFP1) fused at its C-terminus to the human cytoplasmic b-actin. The AcGFP1-Actin fusion protein incorporates into growing actin filaments, allowing visualization of the actin cytoskeleton in living or fixed cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632435
pIRES2-AcGFP1 Vector
20 ug
USD $589.00
pIRES2-AcGFP1 is a bicistronic vector designed for the simultaneous expression of a novel green fluorescent protein (AcGFP1) and a protein of interest from the same transcript in transfected mammalian cells. This vector contains an internal ribosome entry site (IRES2) of the encephalomyocarditis virus (EMCV). When a gene of interest is inserted into the MCS located upstream of the IRES, both the gene of interest and the AcGFP1 coding region will be translated from a single bicistronic mRNA.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632432
pAcGFP1-Mito Vector
20 ug
USD $589.00
pAcGFP1-Mito encodes a humanized green fluorescent protein (GFP) derived from Aequorea coerulescens . A mitochondrial targeting sequence from the precursor protein of human cytochrome c oxidase subunit VIII is fused to the N-terminus of the fluorescent protein. The fluorescence from pAcGFP1-Mito expression can be observed within the mitochondrial matrix inside the inner membrane. pAcGFP1-Mito can be used for specific labeling of mitochondria in living and fixed cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632431
pAcGFP1-Nuc Vector
20 ug
USD $589.00
pAcGFP1-Nuc encodes a humanized green fluorescent protein (GFP) derived from Aequorea coerulescens . The fluorescent protein is fused at its C-terminus to three copies of the nuclear localization signal (NLS) of the SV40 T-antigen. This vector is designed a marker for visualizing the nucleus in living or fixed cells by fluorescence microscopy.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632426
AcGFP1 Vector Set
Each
USD $1130.00
A set of three vectors encoding green fluorescent protein from Aequorea coerulescens . pAcGFP1 is a prokaryotic expression vector that also serves as a convenient source of fluorescent protein cDNA. In this vector, the full-length human-codon-optimized cDNA is flanked by distinct MCSs to facilitate excision from the vector and insertion into other eukaryotic or prokaryotic expression constructs. The other vectors, pAcGFP1-C1 and pAcGFP1-N1, are designed for studies in mammalian systems. These vectors allow expression of a protein of interest as a C- or N-terminal fusion, respectively. The fusion vectors can also be used as cotransfection markers since the unmodified vectors will express fluorescent protein. All of the fluorescent protein coding sequences in these constructs have been human-codon-optimized for efficient expression in mammalian cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632155
pLVX-AcGFP1-C1 Vector
10 ug
USD $676.00
The pLVX-AcGFP1-C1 vector is an HIV-1 based lentiviral expression vector that can produce recombinant lentivirus that efficiently transduces both dividing and non-dividing mammalian cells. This vector expresses a variant of the green fluorescent protein (GFP) from Aequorea coerulescens . pLVX-AcGFP1-C1 allows cloning of a cDNA into the multiple cloning site (MCS) downstream of the coding sequence for AcGFP1. The cDNA is subsequently expressed as a fusion to the C-terminus of the AcGFP1 protein. Thus,
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
632154
pLVX-AcGFP1-N1 Vector
10 ug
USD $676.00
The pLVX-AcGFP1-N1 vector is an HIV-1-based lentiviral expression vector that can produce recombinant lentivirus that efficiently transduces both dividing and non-dividing mammalian cells. This vector expresses a variant of the green fluorescent protein (GFP) from Aequorea coerulescens . pLVX-AcGFP1-N1 allows cloning of a cDNA into the multiple cloning site (MCS) upstream of the coding sequence for AcGFP1. The cDNA is subsequently expressed as a fusion to the N-terminus of the AcGFP1 protein. Thus, this vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable transductants. The unmodified vector will express AcGFP1 protein and may be used to produce marker virus to optimize infection protocols.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631984
pLVX-EF1alpha-AcGFP1-C1 Vector
10 ug
USD $676.00
pLVX-EF1α-AcGFP1-C1 is a lentiviral expression vector that can be used to generate high-titer lentivirus for transducing virtually any dividing or nondividing mammalian cell type, including primary and stem cells. The vector allows a gene-of-interest to be fused to the C-terminus of the green fluorescent protein AcGFP1. Expression of the fusion is driven by the human elongation factor 1 alpha (EF1α) promoter, which continues to be constitutively active even after stable integration of the vector into the host cell genome. Stable expression of the fusion allows the monitoring of a variety of cellular processes (such as differentiation in primary or stem cells) without the transgene silencing associated with CMV promoters. In addition, the vector allows efficient flow cytometric detection of stably or transiently transfected mammalian cells expressing AcGFP1 fusions, without time-consuming drug and clonal selection.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631983
pLVX-EF1alpha-AcGFP1-N1 Vector
10 ug
USD $676.00
pLVX-EF1α-AcGFP1-N1 is a lentiviral expression vector that can be used to generate high-titer lentivirus for transducing virtually any dividing or nondividing mammalian cell type, including primary and stem cells. The vector allows a gene-of-interest to be fused to the N-terminus of the green fluorescent protein AcGFP1. Expression of the fusion is driven by the human elongation factor 1 alpha (EF1α) promoter, which continues to be constitutively active even after stable integration of the vector into the host cell genome. Stable expression of the fusion allows the monitoring of a variety of cellular processes (such as differentiation in primary or stem cells) without the transgene silencing associated with CMV promoters. In addition, the vector allows efficient flow cytometric detection of stably or transiently transfected mammalian cells expressing AcGFP1 fusions, without time-consuming drug and clonal selection.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631974
pEF1alpha-AcGFP1-C1 Vector
10 ug
USD $555.00
pEF1α-AcGFP1-C1 is a mammalian expression vector that constitutively expresses a protein of interest fused to the C-terminus of the green fluorescent protein AcGFP1, even after stable integration of the vector into the host cell genome. Stable, constitutive expression of the bicistronic transcript is driven by the human elongation factor 1 alpha (EF1α) promoter, allowing the monitoring of a variety of cellular processes (such as differentiation in primary or stem cells) without the transgene silencing associated with CMV promoters. The unmodified vector can be used to express modified AcGFP1 in mammalian cells.
Notice to purchaser
Our products are to be used for Research Use Only . They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
631973
pEF1alpha-AcGFP1-N1 Vector
10 ug
USD $555.00
pEF1α-AcGFP1-N1 is a mammalian expression vector that constitutively expresses a protein of interest fused to the N-terminus of the green fluorescent protein AcGFP1, even after stable integration of the vector into the host cell genome. Stable, constitutive expression of the fusion protein is driven by the human elongation factor 1 alpha (EF1α) promoter, allowing the monitoring of a variety of cellular processes (such as differentiation in primary or stem cells) without the transgene silencing associated with CMV promoters. The unmodified vector can be used to express AcGFP1 in mammalian cells.
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