AsRed2 fluorescent protein

pAsRed2 is a prokaryotic expression vector, which encodes a human codon-optimized variant of Anemonia sulcata red fluorescent protein, AsRed2, which has been engineered for brighter fluorescence and higher expression in mammalian cells. In this vector, the AsRed2 gene is flanked by separate and distinct MCS regions at the 5' and 3' ends so that the AsRed2 gene can be subcloned into other expression vectors. This vector is primarily intended to serve as a convenient source of AsRed2 cDNA.

Back

Basic fluorescent protein vector map

Basic fluorescent protein vector map. Use this bacterial expression vector as a source of the fluorescent protein gene. Note: There is a stop codon at the 5' end of the 3' MCS. The 3' MCS should not be used for cloning.

Back

Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1

Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2,  and HcRed1.

pAsRed2-C1 is a human codon-optimized expression vector that encodes a variant of the Anemonia sulcata red fluorescent protein, AsRed2, which has been engineered for brighter fluorescence and higher expression in mammalian cells. pAsRed2-C1 allows genes cloned into the multiple cloning site (MCS) downstream of the AsRed2 coding sequence to be expressed as fusions to the C-terminus of the fluorescent protein. The unmodified vector will express AsRed2 in mammalian cells.

Back

Schematic for fluorescent fusion protein vectors

Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.

pAsRed2-N1 is a human codon-optimized expression vector that encodes a variant of the Anemonia sulcata red fluorescent protein, AsRed2, which has been engineered for brighter fluorescence and higher expression in mammalian cells. pAsRed2-N1 allows genes cloned into the multiple cloning site (MCS) upstream of the AsRed2 coding sequence to be expressed as fusions to the N-terminus of the fluorescent protein. The unmodified vector will express AsRed2 in mammalian cells.

Back

Schematic for fluorescent fusion protein vectors

Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.

Back

Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1

Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2,  and HcRed1.

A set of twelve vectors encoding novel fluorescent proteins in four colors: cyan, green, red and yellow. Four constructs are prokaryotic expression vectors that also serve as convenient sources of fluorescent protein cDNAs. In each of these prokaryotic vectors, the full-length cDNA is flanked by distinct MCSs to facilitate excision from the vector and insertion into other eukaryotic or prokaryotic expression constructs. Eight of the vectors are designed for studies in mammalian systems. These vectors allow expression of a protein of interest as a C- or N-terminal fusion to any of the four fluorescent proteins. The fusion vectors can also be used as cotransfection markers; the unmodified vectors will express fluorescent protein.