E2-Crimson fluorescent protein

The mammalian CMV promoter in pCMV-E2-Crimson allows the constitutive expression of the far-red Living Colors fluorescent protein E2-Crimson, driven by the constitutive immediate early promoter of cytomegalovirus (CMV). pCMV-E2-Crimson does not contain an MCS. It can be used for labeling purposes or as a cotransfection marker to determine transfection efficiency. If required, stable transfectants can be selected using G418.

pE2-Crimson-C1 is a mammalian expression vector designed to express a protein of interest fused to the C-terminus of E2-Crimson, our brightest and fastest-maturing far-red Living Colors fluorescent protein. E2-Crimson was derived from DsRed-Express2, and retains its fast maturation, high photostability, increased solubility, and low cytotoxicity. The unmodified vector can be used to express E2-Crimson in mammalian cells. If required, stable transfectants can be selected using G418.

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Schematic for fluorescent fusion protein vectors

Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.

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E2-Crimson is useful for confocal and STED (stimulated emission depletion) microscopy

E2-Crimson is useful for confocal and STED (stimulated emission depletion) microscopy. The mammalian ER was imaged by conventional confocal microscopy (left) or by STED microscopy (right) with 635 nm excitation and a STED wavelength of 760 nm. The scale bar is 1 μm.

pE2-Crimson-N1 is a mammalian expression vector designed to express a protein of interest fused to the N-terminus of E2-Crimson, our brightest and fastest-maturing far-red Living Colors fluorescent protein. E2-Crimson was derived from DsRed-Express2, and retains its fast maturation, high photostability, increased solubility, and low cytotoxicity. The unmodified vector can be used to express E2-Crimson in mammalian cells. If required, stable transfectants can be selected using G418.

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Schematic for fluorescent fusion protein vectors

Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.

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E2-Crimson is useful for confocal and STED (stimulated emission depletion) microscopy

E2-Crimson is useful for confocal and STED (stimulated emission depletion) microscopy. The mammalian ER was imaged by conventional confocal microscopy (left) or by STED microscopy (right) with 635 nm excitation and a STED wavelength of 760 nm. The scale bar is 1 μm.

pE2-Crimson is a prokaryotic expression vector which encodes E2-Crimson, our brightest and fastest-maturing far-red Living Colors fluorescent protein. E2-Crimson was derived from DsRed-Express2, and retains its fast maturation, high photostability, increased solubility, and low cytotoxicity. pE2-Crimson is primarily intended to serve as a source of E2-Crimson cDNA. The flanking MCS regions make it possible to excise the E2-Crimson coding sequence and insert it into other expression vectors of choice. The vector can also be used in bacteria to produce E2-Crimson protein.

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Basic fluorescent protein vector map

Basic fluorescent protein vector map. Use this bacterial expression vector as a source of the fluorescent protein gene. Note: There is a stop codon at the 5' end of the 3' MCS. The 3' MCS should not be used for cloning.

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E2-Crimson is useful for confocal and STED (stimulated emission depletion) microscopy

E2-Crimson is useful for confocal and STED (stimulated emission depletion) microscopy. The mammalian ER was imaged by conventional confocal microscopy (left) or by STED microscopy (right) with 635 nm excitation and a STED wavelength of 760 nm. The scale bar is 1 μm.

pEF1α-E2-Crimson is a mammalian expression vector that constitutively expresses the far-red fluorescent protein E2-Crimson, even after stable integration of the vector into the host cell genome. Stable, constitutive expression of E2-Crimson is driven by the human elongation factor 1 alpha (EF1α) promoter, which allows the protein to be expressed without the transgene silencing associated with CMV promoters. The vector, which lacks an MCS, is designed to be used for cell labeling or as a marker of transfection efficiency.

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Map schematic of the plasmid choices that are available carrying the EF1-alpha promoter

Map schematic of the plasmid choices that are available carrying the EF1-alpha promoter. IRES: internal ribosome entry sequence; FP1: fluorescent protein (AcGFP1, DsRed-Monomer, or mCherry); FP2: fluorescent protein (mCherry or ZsGreen1); MCS: multiple cloning site.