Wild-type GFP & GFPuv fluorescent proteins

Green fluorescent protein (GFP) is a naturally fluorescent protein from Aequorea victoria that can be used as an in vivo reporter of gene expression and protein localization. pGFP is a convenient source of the GFP cDNA. The GFP coding sequence is flanked by separate MCSs at the 5' and 3' ends, so the GFP gene can be easily be excised. Alternatively, the GFP coding sequences can be amplified by PCR.

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pGFP and pGFP-UV vector maps

pGFP and pGFP-UV vector maps. Note: There is a stop codon at the 5' end of the 3' MCS. The 3' MCS should not be used for cloning.

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632370: pGFP Vector

pGFPuv encodes a variant of Aequorea victoria green fluorescent protein (GFP) that has been optimized for brighter fluorescence when excited by standard UV light. The GFPuv coding sequence is flanked by separate MCSes at the 5' and 3' ends, so the GFPuv gene can be excised from pGFPuv. Alternatively, the GFPuv coding sequences can be amplified by PCR.

pGFPuv carries the "cycle 3" variant of GFP. This gene was cloned between the two MCSs of the pUC19 derivative pPD16.43. The GFPuv gene can be easily excised from pGFPuv. Alternatively, the GFPuv coding sequence can be amplified by PCR. The GFPuv gene was inserted in frame with the lacZ initiation codon from pUC19 so that a β-galactosidase-GFPuv fusion protein is expressed from the lac promoter in E. coli. Note, however, that if you excise the GFPuv coding sequence using a restriction site in the 5' MCS, the resulting fragment will encode the native (i.e., non-fusion) GFPuv protein. The pUC backbone of pGFPuv provides a high copy number origin of replication and ampicillin resistance gene for propagation in E. coli.

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pGFP and pGFP-UV vector maps

pGFP and pGFP-UV vector maps. Note: There is a stop codon at the 5' end of the 3' MCS. The 3' MCS should not be used for cloning.

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632312: pGFPuv Vector