NucleoMag RNA Pro and NucleoMag RNA/DNA Pro
Isolate both RNA and DNA from tissue, plants, or cultured cells—all with one kit. NucleoMag RNA Pro enables rapid and efficient small-scale purification of high-quality RNA and NucleoMag DNA/RNA Pro enables rapid and efficient small-scale purification of high-quality RNA and DNA. Both kits are compatible with manual workflows using NucleoMag SEP or can be automated on magnetic rod systems such as the IsoPure Mini. Processing 96 samples takes 120 min when done manually, and around 90 min with an automated magnetic rod system.
Isolate both RNA and DNA from tissue, plants, or cultured cells—all with one kit. NucleoMag RNA Pro enables rapid and efficient small-scale purification of high-quality RNA and NucleoMag DNA/RNA Pro enables rapid and efficient small-scale purification of high-quality RNA and DNA. Both kits are compatible with manual workflows using NucleoMag SEP or can be automated on magnetic rod systems such as the IsoPure Mini. Processing 96 samples takes 120 min when done manually, and around 90 min with an automated magnetic rod system.
With NucleoMag RNA Pro, sample lysis is achieved by homogenization in a solution containing chaotropic ions. Following sample lysis, ethanol-based solvents are added to the lysate, which creates the proper conditions for nucleic acids to bind to the paramagnetic NucleoMag B-Beads. Contaminants and salts are removed using Wash Buffer MRW and 70% ethanol. After magnetic separation, the NucleoMag B-Beads are incubated with a recombinant DNase to remove co-purified DNA. Following an RNA rebinding step and washing, highly pure RNA is eluted and can be used directly for downstream applications.
NucleoMag RNA/DNA Pro is identical to NucleoMag RNA Pro but contains the buffers needed to isolate DNA in a separate fraction following the removal of contaminants and salts using Wash Buffer MRW and 70% ethanol. The eluted DNA, like the eluted RNA, can be used directly for downstream applications.
Overview
- Have the flexibility to isolate RNA and DNA, or RNA only, from the same sample
- Use one kit for cultured cells, tissue samples, or plants
- Process manually or streamline with automated magnetic rod systems
- Obtain highly pure RNA/DNA that can be used directly in downstream applications such as RT-PCR, enzymatic reactions, and sequencing
More Information
| NucleoMag RNA Pro | NucleoMag RNA/DNA Pro | |
| Format | Magnetic bead | Magnetic bead |
| Target | RNA* | RNA and DNA |
| Sample material | Cells, tissue, and plant material | Cells, tissue, and plant material |
| Sample amounts | Up to 20 mg tissue Up to 2 x 106 cultured cells Up to 40 mg plant leaf material |
Up to 20 mg tissue Up to 2 x 106 cultured cells Up to 40 mg plant leaf material |
| Fragment size | >200 nt | >200 nt |
| Binding capacity | 0.4 μg/μl B-Beads | 0.4 μg/μl B-Beads |
| Automated platform compatibility | Compatible with most open robotic platforms. Please contact technical support for more information. | Compatible with most open robotic platforms. Please contact technical support for more information. |
| Elution volume | 50–200 μl | 50–200 μl |
| Processing time | 90–120 min, depending on manual or automated processing | 90–120 min, depending on manual or automated processing |
*DNA can also be isolated using NucleoMag RNA Pro with the NucleoSpin RNA/DNA Buffer Set (Cat. # 740944). Please see the User Manual for more information.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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