Purify total RNA from fresh or frozen whole blood
NucleoSpin RNA Blood kits are are available in individual prep and 96-well plate formats. Simple and convenient, this procedure allows for effective direct lysis of blood cells at room temperature without an upstream selective erythrocyte lysis at 4°C. Compared to competitor kits, NucleoSpin RNA Blood kits show higher yields from smaller sample volumes. Both fresh and frozen blood samples can be used to purify RNA with comparable yield and quality.
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The better way to isolate RNA
Critical factors when purifying RNA are the complete removal of contaminating DNA (gDNA) and the immediate prevention of degradation of RNA. The NucleoSpin RNA Plus kit introduces the NucleoSpin gDNA Removal Column, a spin column which quickly and completely removes genomic DNA contamination without the need for DNase digestion. To maintain RNA integrity, cells and tissues are first lysed by incubation in a chaotropic ion lysis buffer solution, which immediately inactivates RNases.
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