Complete midiprep kit for purification of total RNA—NucleoSpin RNA Midi
NucleoSpin RNA Midi allows isolation of up to 600 µg of DNA-free, high-quality total RNA from large amounts of starting material such as cultured cells, tissue, bacteria, and yeast without the use of organic compounds (e.g., Trizol). The working procedure is similar to standard RNA purification methods and includes effective rDNase digestion of contaminating DNA during the purification process.
Overview
- Complete midi kit with rDNase for on-column digestion—includes shredders
- Purify total RNA from cells, tissue, and yeast
- Ready-to-use RNA for high performance in any downstream applications
- No use of toxic material such as phenol/chloroform (e.g., Trizol), CsCl, or ethanol
More Information
| Technology | Silica membrane |
| Format | Midi spin columns |
| Starting material |
<5 x 107 cultured cells |
| Fragment size | 200 bp–20 kb |
| Typical yield | 180 µg from 107 HeLa cells 620 µg from 4 x 107 HeLa cells |
| A260/280 | 1.90–2.10 |
| Elution volume | 500 µl |
| Preparation time | 80 min/4 preps |
Applications
Isolate total RNA from cultured cells and tissue (standard protocol), as well as bacterial cells, yeast cells, and samples stored in RNAlater (support protocols).
Purified RNA suitable for:
- Real-time RT-PCR
- Northern blotting
- Primer extension
- Array technology
- RNase protection assays
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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