Mini & midi spin kits for isolation of total RNA from blood—NucleoSpin RNA Blood & NucleoSpin RNA Blood Midi
The NucleoSpin RNA Blood and NucleoSpin RNA Blood Midi kits provide a simple, efficient, and direct total blood lysis method for isolating high-quality RNA from fresh or frozen whole blood that has been stabilized with anticoagulants (e.g., EDTA, citrate, or heparin). The entire procedure is performed at room temperature, with no need for selective erythrocyte lysis at 4°C (a common and cumbersome requirement for other methods).
The NucleoSpin RNA Blood and NucleoSpin RNA Blood Midi kits provide a simple, efficient, and direct total blood lysis method for isolating high-quality RNA from fresh or frozen whole blood that has been stabilized with anticoagulants (e.g., EDTA, citrate, or heparin). The entire procedure is performed at room temperature, with no need for selective erythrocyte lysis at 4°C (a common and cumbersome requirement for other methods). The isolated RNA is suitable for downstream applications such as qRT-PCR, next-generation sequencing, blotting, and array technology.
NucleoSpin RNA Blood
The NucleoSpin RNA Blood kit allows isolation of high-quality RNA from up to 400 µl of fresh or frozen whole blood. After total blood cell lysis in Lysis Buffer DL, the RNA is bound to a NucleoSpin RNA Blood Binding Column. Contaminating DNA and inhibitors are efficiently removed by on-column rDNase digestion and washes with two different wash buffers. The purified RNA is then eluted with RNase-free water.
Compared with competitor kits, NucleoSpin RNA Blood kits show higher yields from smaller sample volumes. In addition, it is possible to obtain a linear increase in yield relative to sample volume. Both fresh and frozen blood samples can be used to purify RNA of comparable yield and quality.
NucleoSpin RNA Blood Midi
The NucleoSpin RNA Blood Midi kit allows isolation of high-quality RNA from up to 1,300 µl of fresh or frozen whole blood. The working procedure is similar to the NucleoSpin RNA Blood method and includes simple, efficient, and direct total blood lysis, as well as effective rDNase digestion of contaminating DNA during the purification process. No selective erythrocyte lysis is needed and the whole procedure can be performed at room temperature.
Overview
- Simple, direct total blood lysis at room temperature
- Convenient handling of different sample volumes and types
- Superior RNA yield and quality from up to 400 µl (or 400–1,300 µl for Midi kits) of whole blood
- Efficient on-column DNA removal provides increased sensitivity in downstream applications
- Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate, and heparin)
More Information
| NucleoSpin RNA Blood | NucleoSpin RNA Blood Midi | |
| Technology | Silica membrane | Silica membrane |
| Format | Mini spin columns | Midi spin columns |
| Starting material | <400 µl whole blood (fresh or frozen) |
400–1,300 µl whole blood |
| Fragment size | >200 nt | >200 nt |
| Typical yield | 1–8 µg (400 µl whole blood)* | 4–26 µg (1,300 µl whole blood)* |
| A260/280 | 1.9–2.1 | 1.9–2.1 |
| Elution volume | 40–120 µl | 200–400 µl |
| Preparation time | 55 min/6 preps | 75 min/6 preps |
| Binding capacity | 200 µg | 700 µg |
*RNA yield strongly depends on the leukocyte number in each individual blood sample.
Applications
- qRT-PCR
- Next-generation sequencing
- Blotting
- Array technology
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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The better way to isolate RNA
Critical factors when purifying RNA are the complete removal of contaminating DNA (gDNA) and the immediate prevention of degradation of RNA. The NucleoSpin RNA Plus kit introduces the NucleoSpin gDNA Removal Column, a spin column which quickly and completely removes genomic DNA contamination without the need for DNase digestion. To maintain RNA integrity, cells and tissues are first lysed by incubation in a chaotropic ion lysis buffer solution, which immediately inactivates RNases.
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