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Nucleic acid purification

Isolation and purification of total RNA from FFPE samples

Extract and purify small (miRNA) and large RNA from formalin-fixed, paraffin-embedded (FFPE) samples

The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS kits provide a fast, efficient, and convenient method for isolating both small (e.g., miRNA) and large RNAs from formalin-fixed, paraffin-embedded (FFPE) tissue.

NucleoSpin totalRNA FFPE includes mini spin columns (30–50 µl elution volume) to purify standard FFPE samples (<50 mg tissue), while NucleoSpin totalRNA FFPE XS includes XS columns (5–30 µl elution volume) to purify very small FFPE samples (<5 mg tissue, e.g., microdissections). Both kits include blue-colored Paraffin Dissolver for easy visualization of phase separation and convenient paraffin removal without using toxic organic solvents (such as xylene), and rDNase for efficient on-column DNA removal. The kits combine decrosslinking and tissue lysis in one step, providing reliable yields of high-quality total RNA for enhanced RT-PCR performance. The NucleoSpin totalRNA FFPE XS kit makes it possible to isolate highly concentrated RNA suitable for qRT-PCR from even trace amounts of FFPE samples.

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Cat. #	Product	Size	Price
740982.10	NucleoSpin® totalRNA FFPE	10 Preps	USD $90.00
The NucleoSpin totalRNA FFPE kit (50 preps) allows for the purification of small and large RNA species from standard FFPE samples (<50 mg tissue) into a 30–50 µl elution volume. The kit includes NucleoSpin RNA Columns, Collection Tubes, Paraffin Dissolver, buffers, Proteinase K, and RNase-free rDNase. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back NucleoSpin totalRNA FFPE provides better RT-PCR performance (lower CT values) than competitor kits, indicating higher RNA yields NucleoSpin totalRNA FFPE provides better RT-PCR performance (lower C_T values) than competitor kits, indicating higher RNA yields. Large (e.g., mRNA) and small (e.g., miRNA) RNA were isolated from 4 x 10 μm FFPE sections of mouse brain tissue with NucleoSpin totalRNA FFPE and three competitor kits (Q, A, P). Quantification of mRNA* and miRNA** was performed using qRT-PCR. Low C_T values indicate high RNA yields. The amplification target consists of a 230-base fragment of the β2-microglobulin gene. miRNA was quantified using the miR-16 MicroRNA Assay in the TaqMan MicroRNA Reverse Transcription Kit from Applied Biosystems. Back NucleoSpin totalRNA FFPE provides more efficient gDNA removal than competitor kits NucleoSpin totalRNA FFPE provides more efficient gDNA removal than competitor kits.* Large (e.g., mRNA) and small (e.g., miRNA) RNA were isolated from 4 x 10 μm FFPE sections of mouse brain tissue with NucleoSpin totalRNA FFPE and three competitor kits (Q, A, P). Residual DNA was assayed by amplifying a 191-bp fragment of the mGAPDH gene. Higher C_T values correspond to lower amounts of residual DNA. Back The higher concentration of total RNA obtained from XS columns allows efficient, sensitive qRT-PCR from very small FFPE samples The higher concentration of total RNA obtained from XS columns allows efficient, sensitive qRT-PCR from very small FFPE samples. Trace amounts of FFPE samples (1 x 15 μm mouse spleen and 1 x 10 μm mouse heart) were used to isolate mRNA and miRNA with NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS. The RNA was eluted in 50 μl (using a NucleoSpin totalRNA FFPE mini spin column) and 20 μl (using a NucleoSpin totalRNA FFPE XS column). The mRNA* and miRNA** eluted from the XS column at higher concentrations, reducing the C_T value by at least one cycle. When using standard FFPE sample amounts, NucleoSpin totalRNA FFPE (which uses mini spin columns) is recommended in order to avoid possible column overloading. The amplification target consists of a 230-base fragment of the β2-microglobulin gene. miRNA was quantified using the miR-16 MicroRNA Assay in the TaqMan MicroRNA Reverse Transcription Kit from Applied Biosystems Back The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS procedures are the same for both kits The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS procedures are the same for both kits.* They only differ in that NucleoSpin totalRNA FFPE uses mini spin columns which elute in 30–50 µl to purify standard FFPE samples (<50 mg tissue), while NucleoSpin totalRNA FFPE XS uses XS columns which elute in 5–30 µl to purify very small FFPE samples (< 5 mg tissue). Back 740982.10: NucleoSpin totalRNA FFPE
740982.50	NucleoSpin® totalRNA FFPE	50 Preps	USD $407.00
The NucleoSpin totalRNA FFPE kit (50 preps) allows for the purification of small and large RNA species from standard FFPE samples (<50 mg tissue) into a 30–50 µl elution volume. The kit includes NucleoSpin RNA Columns, Collection Tubes, Paraffin Dissolver, buffers, Proteinase K, and RNase-free rDNase. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back NucleoSpin totalRNA FFPE provides better RT-PCR performance (lower CT values) than competitor kits, indicating higher RNA yields NucleoSpin totalRNA FFPE provides better RT-PCR performance (lower C_T values) than competitor kits, indicating higher RNA yields. Large (e.g., mRNA) and small (e.g., miRNA) RNA were isolated from 4 x 10 μm FFPE sections of mouse brain tissue with NucleoSpin totalRNA FFPE and three competitor kits (Q, A, P). Quantification of mRNA* and miRNA** was performed using qRT-PCR. Low C_T values indicate high RNA yields. The amplification target consists of a 230-base fragment of the β2-microglobulin gene. miRNA was quantified using the miR-16 MicroRNA Assay in the TaqMan MicroRNA Reverse Transcription Kit from Applied Biosystems. Back NucleoSpin totalRNA FFPE provides more efficient gDNA removal than competitor kits NucleoSpin totalRNA FFPE provides more efficient gDNA removal than competitor kits.* Large (e.g., mRNA) and small (e.g., miRNA) RNA were isolated from 4 x 10 μm FFPE sections of mouse brain tissue with NucleoSpin totalRNA FFPE and three competitor kits (Q, A, P). Residual DNA was assayed by amplifying a 191-bp fragment of the mGAPDH gene. Higher C_T values correspond to lower amounts of residual DNA. Back The higher concentration of total RNA obtained from XS columns allows efficient, sensitive qRT-PCR from very small FFPE samples The higher concentration of total RNA obtained from XS columns allows efficient, sensitive qRT-PCR from very small FFPE samples. Trace amounts of FFPE samples (1 x 15 μm mouse spleen and 1 x 10 μm mouse heart) were used to isolate mRNA and miRNA with NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS. The RNA was eluted in 50 μl (using a NucleoSpin totalRNA FFPE mini spin column) and 20 μl (using a NucleoSpin totalRNA FFPE XS column). The mRNA* and miRNA** eluted from the XS column at higher concentrations, reducing the C_T value by at least one cycle. When using standard FFPE sample amounts, NucleoSpin totalRNA FFPE (which uses mini spin columns) is recommended in order to avoid possible column overloading. The amplification target consists of a 230-base fragment of the β2-microglobulin gene. miRNA was quantified using the miR-16 MicroRNA Assay in the TaqMan MicroRNA Reverse Transcription Kit from Applied Biosystems Back The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS procedures are the same for both kits The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS procedures are the same for both kits.* They only differ in that NucleoSpin totalRNA FFPE uses mini spin columns which elute in 30–50 µl to purify standard FFPE samples (<50 mg tissue), while NucleoSpin totalRNA FFPE XS uses XS columns which elute in 5–30 µl to purify very small FFPE samples (< 5 mg tissue). Back 740982.50: NucleoSpin totalRNA FFPE
740982.250	NucleoSpin® totalRNA FFPE	250 Preps	USD $1780.00
The NucleoSpin totalRNA FFPE kit (250 preps) allows for the purification of small and large RNA species from standard FFPE samples (<50 mg tissue) into a 30–50 µl elution volume. The kit includes NucleoSpin RNA Columns, Collection Tubes, Paraffin Dissolver, buffers, Proteinase K, and RNase-free rDNase. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back NucleoSpin totalRNA FFPE provides better RT-PCR performance (lower CT values) than competitor kits, indicating higher RNA yields NucleoSpin totalRNA FFPE provides better RT-PCR performance (lower C_T values) than competitor kits, indicating higher RNA yields. Large (e.g., mRNA) and small (e.g., miRNA) RNA were isolated from 4 x 10 μm FFPE sections of mouse brain tissue with NucleoSpin totalRNA FFPE and three competitor kits (Q, A, P). Quantification of mRNA* and miRNA** was performed using qRT-PCR. Low C_T values indicate high RNA yields. The amplification target consists of a 230-base fragment of the β2-microglobulin gene. miRNA was quantified using the miR-16 MicroRNA Assay in the TaqMan MicroRNA Reverse Transcription Kit from Applied Biosystems. Back NucleoSpin totalRNA FFPE provides more efficient gDNA removal than competitor kits NucleoSpin totalRNA FFPE provides more efficient gDNA removal than competitor kits.* Large (e.g., mRNA) and small (e.g., miRNA) RNA were isolated from 4 x 10 μm FFPE sections of mouse brain tissue with NucleoSpin totalRNA FFPE and three competitor kits (Q, A, P). Residual DNA was assayed by amplifying a 191-bp fragment of the mGAPDH gene. Higher C_T values correspond to lower amounts of residual DNA. Back The higher concentration of total RNA obtained from XS columns allows efficient, sensitive qRT-PCR from very small FFPE samples The higher concentration of total RNA obtained from XS columns allows efficient, sensitive qRT-PCR from very small FFPE samples. Trace amounts of FFPE samples (1 x 15 μm mouse spleen and 1 x 10 μm mouse heart) were used to isolate mRNA and miRNA with NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS. The RNA was eluted in 50 μl (using a NucleoSpin totalRNA FFPE mini spin column) and 20 μl (using a NucleoSpin totalRNA FFPE XS column). The mRNA* and miRNA** eluted from the XS column at higher concentrations, reducing the C_T value by at least one cycle. When using standard FFPE sample amounts, NucleoSpin totalRNA FFPE (which uses mini spin columns) is recommended in order to avoid possible column overloading. The amplification target consists of a 230-base fragment of the β2-microglobulin gene. miRNA was quantified using the miR-16 MicroRNA Assay in the TaqMan MicroRNA Reverse Transcription Kit from Applied Biosystems Back The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS procedures are the same for both kits The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS procedures are the same for both kits.* They only differ in that NucleoSpin totalRNA FFPE uses mini spin columns which elute in 30–50 µl to purify standard FFPE samples (<50 mg tissue), while NucleoSpin totalRNA FFPE XS uses XS columns which elute in 5–30 µl to purify very small FFPE samples (< 5 mg tissue). Back 740982.250: NucleoSpin totalRNA FFPE
740969.10	NucleoSpin® totalRNA FFPE XS	10 Preps	USD $98.00
The NucleoSpin totalRNA FFPE kit (50 preps) allows for the purification of small and large RNA species from very small FFPE samples (<5 mg tissue) into a 5–30 µl elution volume. The kit includes NucleoSpin RNA FFPE XS Columns, Collection Tubes, Paraffin Dissolver, buffers, Proteinase K, and RNase-free rDNase. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back NucleoSpin totalRNA FFPE provides better RT-PCR performance (lower CT values) than competitor kits, indicating higher RNA yields NucleoSpin totalRNA FFPE provides better RT-PCR performance (lower C_T values) than competitor kits, indicating higher RNA yields. Large (e.g., mRNA) and small (e.g., miRNA) RNA were isolated from 4 x 10 μm FFPE sections of mouse brain tissue with NucleoSpin totalRNA FFPE and three competitor kits (Q, A, P). Quantification of mRNA* and miRNA** was performed using qRT-PCR. Low C_T values indicate high RNA yields. The amplification target consists of a 230-base fragment of the β2-microglobulin gene. miRNA was quantified using the miR-16 MicroRNA Assay in the TaqMan MicroRNA Reverse Transcription Kit from Applied Biosystems. Back NucleoSpin totalRNA FFPE provides more efficient gDNA removal than competitor kits NucleoSpin totalRNA FFPE provides more efficient gDNA removal than competitor kits.* Large (e.g., mRNA) and small (e.g., miRNA) RNA were isolated from 4 x 10 μm FFPE sections of mouse brain tissue with NucleoSpin totalRNA FFPE and three competitor kits (Q, A, P). Residual DNA was assayed by amplifying a 191-bp fragment of the mGAPDH gene. Higher C_T values correspond to lower amounts of residual DNA. Back The higher concentration of total RNA obtained from XS columns allows efficient, sensitive qRT-PCR from very small FFPE samples The higher concentration of total RNA obtained from XS columns allows efficient, sensitive qRT-PCR from very small FFPE samples. Trace amounts of FFPE samples (1 x 15 μm mouse spleen and 1 x 10 μm mouse heart) were used to isolate mRNA and miRNA with NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS. The RNA was eluted in 50 μl (using a NucleoSpin totalRNA FFPE mini spin column) and 20 μl (using a NucleoSpin totalRNA FFPE XS column). The mRNA* and miRNA** eluted from the XS column at higher concentrations, reducing the C_T value by at least one cycle. When using standard FFPE sample amounts, NucleoSpin totalRNA FFPE (which uses mini spin columns) is recommended in order to avoid possible column overloading. The amplification target consists of a 230-base fragment of the β2-microglobulin gene. miRNA was quantified using the miR-16 MicroRNA Assay in the TaqMan MicroRNA Reverse Transcription Kit from Applied Biosystems Back The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS procedures are the same for both kits The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS procedures are the same for both kits.* They only differ in that NucleoSpin totalRNA FFPE uses mini spin columns which elute in 30–50 µl to purify standard FFPE samples (<50 mg tissue), while NucleoSpin totalRNA FFPE XS uses XS columns which elute in 5–30 µl to purify very small FFPE samples (< 5 mg tissue). Back NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes. Panel A. The columns are optimized to hold a very small silica membrane, yet fit inside a standard 1.5 ml microfuge tube. Panel B. The exceptionally small (2.0 mm) diameter of the NucleoSpinXS membrane (Column MN) allows efficient elution in only 5–30 μl. It provides a significant advantage over the small-sample column offered by Competitor Q (Column Q; 4.8 mm diameter), which requires an elution volume of at least 10–30 μl. Back 740969.10: NucleoSpin totalRNA FFPE XS
740969.50	NucleoSpin® totalRNA FFPE XS	50 Preps	USD $430.00
The NucleoSpin totalRNA FFPE kit (50 preps) allows for the purification of small and large RNA species from very small FFPE samples (<5 mg tissue) into a 5–30 µl elution volume. The kit includes NucleoSpin RNA FFPE XS Columns, Collection Tubes, Paraffin Dissolver, buffers, Proteinase K, and RNase-free rDNase. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back NucleoSpin totalRNA FFPE provides better RT-PCR performance (lower CT values) than competitor kits, indicating higher RNA yields NucleoSpin totalRNA FFPE provides better RT-PCR performance (lower C_T values) than competitor kits, indicating higher RNA yields. Large (e.g., mRNA) and small (e.g., miRNA) RNA were isolated from 4 x 10 μm FFPE sections of mouse brain tissue with NucleoSpin totalRNA FFPE and three competitor kits (Q, A, P). Quantification of mRNA* and miRNA** was performed using qRT-PCR. Low C_T values indicate high RNA yields. The amplification target consists of a 230-base fragment of the β2-microglobulin gene. miRNA was quantified using the miR-16 MicroRNA Assay in the TaqMan MicroRNA Reverse Transcription Kit from Applied Biosystems. Back NucleoSpin totalRNA FFPE provides more efficient gDNA removal than competitor kits NucleoSpin totalRNA FFPE provides more efficient gDNA removal than competitor kits.* Large (e.g., mRNA) and small (e.g., miRNA) RNA were isolated from 4 x 10 μm FFPE sections of mouse brain tissue with NucleoSpin totalRNA FFPE and three competitor kits (Q, A, P). Residual DNA was assayed by amplifying a 191-bp fragment of the mGAPDH gene. Higher C_T values correspond to lower amounts of residual DNA. Back The higher concentration of total RNA obtained from XS columns allows efficient, sensitive qRT-PCR from very small FFPE samples The higher concentration of total RNA obtained from XS columns allows efficient, sensitive qRT-PCR from very small FFPE samples. Trace amounts of FFPE samples (1 x 15 μm mouse spleen and 1 x 10 μm mouse heart) were used to isolate mRNA and miRNA with NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS. The RNA was eluted in 50 μl (using a NucleoSpin totalRNA FFPE mini spin column) and 20 μl (using a NucleoSpin totalRNA FFPE XS column). The mRNA* and miRNA** eluted from the XS column at higher concentrations, reducing the C_T value by at least one cycle. When using standard FFPE sample amounts, NucleoSpin totalRNA FFPE (which uses mini spin columns) is recommended in order to avoid possible column overloading. The amplification target consists of a 230-base fragment of the β2-microglobulin gene. miRNA was quantified using the miR-16 MicroRNA Assay in the TaqMan MicroRNA Reverse Transcription Kit from Applied Biosystems Back The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS procedures are the same for both kits The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS procedures are the same for both kits.* They only differ in that NucleoSpin totalRNA FFPE uses mini spin columns which elute in 30–50 µl to purify standard FFPE samples (<50 mg tissue), while NucleoSpin totalRNA FFPE XS uses XS columns which elute in 5–30 µl to purify very small FFPE samples (< 5 mg tissue). Back NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes. Panel A. The columns are optimized to hold a very small silica membrane, yet fit inside a standard 1.5 ml microfuge tube. Panel B. The exceptionally small (2.0 mm) diameter of the NucleoSpinXS membrane (Column MN) allows efficient elution in only 5–30 μl. It provides a significant advantage over the small-sample column offered by Competitor Q (Column Q; 4.8 mm diameter), which requires an elution volume of at least 10–30 μl. Back 740969.50: NucleoSpin totalRNA FFPE XS
740969.250	NucleoSpin® totalRNA FFPE XS	250 Preps	USD $1895.00
The NucleoSpin totalRNA FFPE kit (250 preps) allows for the purification of small and large RNA species from very small FFPE samples (<5 mg tissue) into a 5–30 µl elution volume. The kit includes NucleoSpin RNA FFPE XS Columns, Collection Tubes, Paraffin Dissolver, buffers, Proteinase K, and RNase-free rDNase. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back NucleoSpin totalRNA FFPE provides better RT-PCR performance (lower CT values) than competitor kits, indicating higher RNA yields NucleoSpin totalRNA FFPE provides better RT-PCR performance (lower C_T values) than competitor kits, indicating higher RNA yields. Large (e.g., mRNA) and small (e.g., miRNA) RNA were isolated from 4 x 10 μm FFPE sections of mouse brain tissue with NucleoSpin totalRNA FFPE and three competitor kits (Q, A, P). Quantification of mRNA* and miRNA** was performed using qRT-PCR. Low C_T values indicate high RNA yields. The amplification target consists of a 230-base fragment of the β2-microglobulin gene. miRNA was quantified using the miR-16 MicroRNA Assay in the TaqMan MicroRNA Reverse Transcription Kit from Applied Biosystems. Back NucleoSpin totalRNA FFPE provides more efficient gDNA removal than competitor kits NucleoSpin totalRNA FFPE provides more efficient gDNA removal than competitor kits.* Large (e.g., mRNA) and small (e.g., miRNA) RNA were isolated from 4 x 10 μm FFPE sections of mouse brain tissue with NucleoSpin totalRNA FFPE and three competitor kits (Q, A, P). Residual DNA was assayed by amplifying a 191-bp fragment of the mGAPDH gene. Higher C_T values correspond to lower amounts of residual DNA. Back The higher concentration of total RNA obtained from XS columns allows efficient, sensitive qRT-PCR from very small FFPE samples The higher concentration of total RNA obtained from XS columns allows efficient, sensitive qRT-PCR from very small FFPE samples. Trace amounts of FFPE samples (1 x 15 μm mouse spleen and 1 x 10 μm mouse heart) were used to isolate mRNA and miRNA with NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS. The RNA was eluted in 50 μl (using a NucleoSpin totalRNA FFPE mini spin column) and 20 μl (using a NucleoSpin totalRNA FFPE XS column). The mRNA* and miRNA** eluted from the XS column at higher concentrations, reducing the C_T value by at least one cycle. When using standard FFPE sample amounts, NucleoSpin totalRNA FFPE (which uses mini spin columns) is recommended in order to avoid possible column overloading. The amplification target consists of a 230-base fragment of the β2-microglobulin gene. miRNA was quantified using the miR-16 MicroRNA Assay in the TaqMan MicroRNA Reverse Transcription Kit from Applied Biosystems Back The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS procedures are the same for both kits The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS procedures are the same for both kits.* They only differ in that NucleoSpin totalRNA FFPE uses mini spin columns which elute in 30–50 µl to purify standard FFPE samples (<50 mg tissue), while NucleoSpin totalRNA FFPE XS uses XS columns which elute in 5–30 µl to purify very small FFPE samples (< 5 mg tissue). Back NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes. Panel A. The columns are optimized to hold a very small silica membrane, yet fit inside a standard 1.5 ml microfuge tube. Panel B. The exceptionally small (2.0 mm) diameter of the NucleoSpinXS membrane (Column MN) allows efficient elution in only 5–30 μl. It provides a significant advantage over the small-sample column offered by Competitor Q (Column Q; 4.8 mm diameter), which requires an elution volume of at least 10–30 μl. Back 740969.250: NucleoSpin totalRNA FFPE XS
740968.25	Paraffin Dissolver	25 mL	USD $37.00
Paraffin Dissolver is an odorless, xylene-free reagent that enables efficient sample deparaffinization and is included with corresponding NucleoSpin and NucleoMag kits for purification of DNA or RNA from FFPE tissues. Cat. # 740968.25 consists of a bottle containing 25 ml of Paraffin Dissolver reagent. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740968.25: Paraffin Dissolver
740343.60	Paraffin Dissolver (blue)	60 mL	USD $78.00
Paraffin Dissolver (blue) is an odorless, xylene-free reagent that enables efficient sample deparaffinization for purification of DNA or RNA from FFPE tissues. Cat. # 740343.60 consists of a bottle containing 60 ml of Paraffin Dissolver (blue) reagent. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740343.60: Paraffin Dissolver (blue)

Overview

Efficient isolation of total RNA from FFPE samples
Two kit options—high recovery from standard and very small FFPE samples
Convenient paraffin removal—no xylene required
Enhanced RT-PCR performance

More Information

	NucleoSpin totalRNA FFPE	NucleoSpin totalRNA FFPE XS
Technology	Silica membrane	Silica membrane
Format	Mini spin columns	XS spin columns
Starting material	4–10 sections (10 µm) of up to 6 mm² total area <50 mg tissue	1–3 sections (10 µm) of up to 6 mm² total area <5 mg tissue
Typical yield	Depends on the amount and quality of the sample	Depends on the amount and quality of the sample
Elution volume	30–50 µl	5–30 µl
Preparation time	70 min/6 preps (90 min including optional rDNase digest)	70 min/6 preps (90 min including optional rDNase digest)
Binding capacity	200 µg	100 µg

Applications

Rapid isolation of RNA from formalin-fixed, paraffin-embedded samples, e.g.:
- Colon carcinoma (unstained or hematoxylin-stained)
- Colon mucosa
- Adenocarcinoma of the colon transversum
- Liver (rat/human)
- Liver carcinoma (hematoxylin-stained)
- Lymph node
- Spleen (rat/human)
- Pancreas (rat/human; diabetic/non-diabetic)
- Placenta
Isolation of RNA from fresh and archived FFPE samples
Isolation of RNA from specimens on object slides (stained or unstained)
Typical downstream application: RT-PCR

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

RNA purification mini prep kits that stop RNA degradation and prevent genomic DNA contamination

The better way to isolate RNA

Critical factors when purifying RNA are the complete removal of contaminating DNA (gDNA) and the immediate prevention of degradation of RNA. The NucleoSpin RNA Plus kit introduces the NucleoSpin gDNA Removal Column, a spin column which quickly and completely removes genomic DNA contamination without the need for DNase digestion. To maintain RNA integrity, cells and tissues are first lysed by incubation in a chaotropic ion lysis buffer solution, which immediately inactivates RNases.

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Isolation and purification of total RNA from FFPE samples

Notice to purchaser

NucleoSpin totalRNA FFPE provides better RT-PCR performance (lower CT values) than competitor kits, indicating higher RNA yields

NucleoSpin totalRNA FFPE provides more efficient gDNA removal than competitor kits

The higher concentration of total RNA obtained from XS columns allows efficient, sensitive qRT-PCR from very small FFPE samples

The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS procedures are the same for both kits

740982.10: NucleoSpin totalRNA FFPE

Notice to purchaser

NucleoSpin totalRNA FFPE provides better RT-PCR performance (lower CT values) than competitor kits, indicating higher RNA yields

NucleoSpin totalRNA FFPE provides more efficient gDNA removal than competitor kits

The higher concentration of total RNA obtained from XS columns allows efficient, sensitive qRT-PCR from very small FFPE samples

The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS procedures are the same for both kits

740982.50: NucleoSpin totalRNA FFPE

Notice to purchaser

NucleoSpin totalRNA FFPE provides better RT-PCR performance (lower CT values) than competitor kits, indicating higher RNA yields

NucleoSpin totalRNA FFPE provides more efficient gDNA removal than competitor kits

The higher concentration of total RNA obtained from XS columns allows efficient, sensitive qRT-PCR from very small FFPE samples

The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS procedures are the same for both kits

740982.250: NucleoSpin totalRNA FFPE

Notice to purchaser

NucleoSpin totalRNA FFPE provides better RT-PCR performance (lower CT values) than competitor kits, indicating higher RNA yields

NucleoSpin totalRNA FFPE provides more efficient gDNA removal than competitor kits

The higher concentration of total RNA obtained from XS columns allows efficient, sensitive qRT-PCR from very small FFPE samples

The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS procedures are the same for both kits

NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes

740969.10: NucleoSpin totalRNA FFPE XS

Notice to purchaser

NucleoSpin totalRNA FFPE provides better RT-PCR performance (lower CT values) than competitor kits, indicating higher RNA yields

NucleoSpin totalRNA FFPE provides more efficient gDNA removal than competitor kits

The higher concentration of total RNA obtained from XS columns allows efficient, sensitive qRT-PCR from very small FFPE samples

The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS procedures are the same for both kits

NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes

740969.50: NucleoSpin totalRNA FFPE XS

Notice to purchaser

NucleoSpin totalRNA FFPE provides better RT-PCR performance (lower CT values) than competitor kits, indicating higher RNA yields

NucleoSpin totalRNA FFPE provides more efficient gDNA removal than competitor kits

The higher concentration of total RNA obtained from XS columns allows efficient, sensitive qRT-PCR from very small FFPE samples

The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS procedures are the same for both kits

NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes

740969.250: NucleoSpin totalRNA FFPE XS

Notice to purchaser

740968.25: Paraffin Dissolver

Notice to purchaser

740343.60: Paraffin Dissolver (blue)

Overview

More Information

Applications