Isolation of genomic DNA from food & feed using magnetic beads—NucleoMag DNA Food

Rapid manual and automated small-scale preparation of DNA from food or feed samples using magnetic beads.

The NucleoMag DNA Food kit is designed for rapid manual and automated small-scale preparation of DNA from <200 mg food or feed samples of plant or animal origin, with typical yields ranging from 0.1–10 μg of pure DNA. The procedure is based on reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions. Magnetic separation can be performed either manually using the NucleoMag SEP magnetic separator or automated on standard liquid handling instruments and automated magnetic separators. Expand the "Details" section of the product table below and select "Image Data" to view data generated with the kit using various food types as inputs.

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Cat. #	Product	Size	Price
744945.1	NucleoMag® DNA Food	1 x 96 Preps	USD $374.00
NucleoMag DNA Food is designed for rapid manual or automated small-scale isolation of genomic DNA from up to 200 mg of food or feed samples of plant or animal origin, with typical yields ranging from 0.1–10 μg of pure DNA. The procedure is based on reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions. Magnetic separation can be performed either manually using the NucleoMag SEP magnetic separator or on an automated platform using standard liquid handling instruments and magnetic separators. Manual preparation of 96 samples in parallel can be completed in approximately 2 hours using NucleoMag SEP. Automated preparation of 96 samples can be completed in less than 2 hours depending on the configuration of the automation platform and the separator being used. Cat. # 744945.1 includes sufficient quantities of reagents and beads for processing of 96 samples. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back NucleoMag DNA Food procedure NucleoMag DNA Food procedure. Back NucleoMag DNA Food can be used to isolate DNA from various food sample types NucleoMag DNA Food can be used to isolate DNA from various food sample types. With NucleoMag DNA Food, a wide variety of food samples can be used as inputs for DNA isolation. For each sample type included in the graph, sample homogenization and lysis were performed manually, whereas DNA isolation was automated using a KingFisher Flex. The presence of DNA in the resulting eluates was determined using qPCR. Back Product comparison demonstrates superior performance of NucleoMag DNA Food Product comparison demonstrates superior performance of NucleoMag DNA Food. DNA was extracted from each of the indicated sample types in parallel using NucleoMag DNA Food and competitor kit "P". For each extraction, sample homogenization and lysis were performed manually, whereas DNA isolation was automated using a KingFisher Flex. The presence of DNA in the resulting eluates was determined using qPCR. For each sample type processed, NucleoMag DNA Food provided superior performance relative to kit "P", as indicated by the lower C_T values for the DNA samples obtained with NucleoMag DNA Food. Back 744945.1: NucleoMag DNA Food
744945.4	NucleoMag® DNA Food	4 x 96 Preps	USD $1270.00
NucleoMag DNA Food is designed for rapid manual or automated small-scale isolation of genomic DNA from up to 200 mg of food or feed samples of plant or animal origin, with typical yields ranging from 0.1–10 μg of pure DNA. The procedure is based on reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions. Magnetic separation can be performed either manually using the NucleoMag SEP magnetic separator or on an automated platform using standard liquid handling instruments and magnetic separators. Manual preparation of 96 samples in parallel can be completed in approximately 2 hours using NucleoMag SEP. Automated preparation of 96 samples can be completed in less than 2 hours depending on the configuration of the automation platform and the separator being used. Cat. # 744945.4 includes sufficient quantities of reagents and beads for processing 4 x 96 samples. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back NucleoMag DNA Food procedure NucleoMag DNA Food procedure. Back NucleoMag DNA Food can be used to isolate DNA from various food sample types NucleoMag DNA Food can be used to isolate DNA from various food sample types. With NucleoMag DNA Food, a wide variety of food samples can be used as inputs for DNA isolation. For each sample type included in the graph, sample homogenization and lysis were performed manually, whereas DNA isolation was automated using a KingFisher Flex. The presence of DNA in the resulting eluates was determined using qPCR. Back Product comparison demonstrates superior performance of NucleoMag DNA Food Product comparison demonstrates superior performance of NucleoMag DNA Food. DNA was extracted from each of the indicated sample types in parallel using NucleoMag DNA Food and competitor kit "P". For each extraction, sample homogenization and lysis were performed manually, whereas DNA isolation was automated using a KingFisher Flex. The presence of DNA in the resulting eluates was determined using qPCR. For each sample type processed, NucleoMag DNA Food provided superior performance relative to kit "P", as indicated by the lower C_T values for the DNA samples obtained with NucleoMag DNA Food. Back 744945.4: NucleoMag DNA Food

Overview

Purify DNA from food and feed for species identification and GMO detection
Isolate even small amounts of partially degraded DNA from complex matrices
Extract DNA from contaminating bacteria
Process a wide variety of samples using flexible kit chemistry

More Information

Technology	Magnetic beads
Format	Highly reactive superparamagnetic beads
Processing	Manual or automated
Starting material	<200 mg food or feed
Fragment size	300 bp–approx. 50 kb
Typical yield	0.1–10 μg
A₂₆₀/A₂₈₀	1.6–1.9
Elution volume	50–200 µl
Preparation time	120 min/96 preps (excl. lysis)
Binding capacity	0.4 µg/µl beads

Applications

Manual or automated isolation of DNA from food and feed
Identification of GMO DNA or animal components
Extraction of DNA from contaminating bacteria
Typical downstream application: real-time PCR

Isolation of viral RNA and DNA and bacterial DNA from cell-free body fluids,homogenized tissue sample suspensions, stool sample suspensions, and swab washes

RNA and DNA isolation from pathogens

The NucleoMag Pathogen kit is designed for the rapid manual and automated small-scale isolation of viral RNA and DNA and bacterial DNA from cell-free body fluids such as serum or plasma, blood, homogenized tissue sample suspensions, stool sample suspensions, and swab washes.

NucleoMag Pathogen NucleoMag SEP magnetic separator

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Isolation of genomic DNA from food & feed using magnetic beads—NucleoMag DNA Food

Notice to purchaser

NucleoMag DNA Food procedure

NucleoMag DNA Food can be used to isolate DNA from various food sample types

Product comparison demonstrates superior performance of NucleoMag DNA Food

744945.1: NucleoMag DNA Food