Isolation of genomic DNA from food & feed—NucleoSpin Food
The NucleoSpin Food kit allows you to quickly isolate genomic DNA from many types of processed food or animal feed of plant or animal origin. Food samples are highly heterogeneous and contain many different compounds such as fats and polysaccharides. These characteristics make DNA extraction more difficult. The NucleoSpin Food protocol guarantees good recovery rates for small genomic DNA fragments from food.
The NucleoSpin Food kit allows you to quickly isolate genomic DNA from many types of processed food or animal feed of plant or animal origin. Food samples are highly heterogeneous and contain many different compounds such as fats and polysaccharides. These characteristics make DNA extraction more difficult. The NucleoSpin Food protocol guarantees good recovery rates for small genomic DNA fragments from food. We have optimized the lysis buffer and purification process to extract DNA from a variety of food products. The purified DNA is ready for use in all types of subsequent detection methods, especially as a template for PCR. Isolation of genomic DNA from food products assists in detecting and identifying contamination, genetically modified organisms (GMOs), or individual plant or animal species.
The NucleoSpin Food kit allows you to process 50–200 mg of homogenized material using a microcentrifuge to speed up the loading, washing, and elution steps. Due to the low DNA content of processed food, we recommend using 200 mg of starting material, which typically yields 0.1–10 µg of DNA. The silica-membrane spin technology has been optimized for high DNA recovery and low binding of impurities, so the kit generates pure, ready-to-use DNA from any food type.
Overview
- Complete removal of PCR inhibitors
- Purify low amounts of partially degraded DNA
- Fast and easy procedure
- Isolate DNA from various types of samples
More Information
| Technology | Silica membrane |
| Format | Mini spin columns |
| Starting material | 5–200 mg |
| Fragment size | 300 bp–30 kb |
| Typical yield | 0.1–10 µg |
| A260/280 | 1.6–1.9 |
| Elution volume | 100 µl |
| Preparation time | 30 min/6 preps |
| Binding capacity | 30 µg |
Applications
- Isolate DNA from complex matrices such as:
- Processed food
- Soy (milk and flour)
- Chocolate
- Cereals
- Meat
- Animal feed
- Purified DNA is suitable for:
- Real-time PCR
- Southern blotting
- Enzymatic reactions
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Designed for extra small samples
Purify DNA from small samples such as microdissected tissue, small blood samples, or small amounts of cells. Based on reliable silica-membrane technology, NucleoSpin Tissue XS overcomes the limitations of conventional kits using an innovative new column design with a funnel-shaped ring.
Learn more All genomic DNA kitsTakara Bio USA, Inc.
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