Rapid and sensitive mycoplasma extraction—NucleoSpin Mycoplasma DNA
Successful extraction of even the smallest traces of mycoplasma DNA from cell culture material is possible with the highly sensitive NucleoSpin Mycoplasma DNA kit. Mycoplasma—a widespread and notoriously undetectable cell culture contaminant—is one of the greatest challenges for scientists performing cell culture. Mycoplasma contamination can significantly impact the integrity and reliability of cell-based assays and experiments, leading to unreliable research outcomes and potentially endangering the safety and efficacy of biopharmaceutical products.
Successful extraction of even the smallest traces of mycoplasma DNA from cell culture material is possible with the highly sensitive NucleoSpin Mycoplasma DNA kit. Mycoplasma—a widespread and notoriously undetectable cell culture contaminant—is one of the greatest challenges for scientists performing cell culture. Mycoplasma contamination can significantly impact the integrity and reliability of cell-based assays and experiments, leading to unreliable research outcomes and potentially endangering the safety and efficacy of biopharmaceutical products.
With NucleoSpin Mycoplasma DNA, mycoplasmas are lysed quickly and efficiently by Lysis Buffer SML, which is a highly concentrated solution of chaotropic ions. Lysis buffer and ethanol create appropriate binding conditions to bind nucleic acids to the NucleoSpin Mycoplasma DNA Columns. Carrier RNA improves binding and recovery of low-concentrated nucleic acids. Contaminants (potential PCR inhibitors) like salts, metabolites, and soluble macromolecular cellular components are removed in simple wash steps with alcoholic buffers SMW1 and SMW2. Then, nucleic acids are eluted in water and are ready for use in subsequent reactions. Every kit lot is tested for nondetectability of mycoplasma DNA, ensuring no mycoplasma DNA background within the kit.
DNA extracted with the kit is suitable for qPCR-based mycoplasma DNA contamination testing. For a complete mycoplasma testing solution, we recommend pairing this kit with the TaKaRa Mycoplasma qPCR Detection Kit.
Overview
- Extract even trace amounts of mycoplasma DNA successfully from cell culture samples
- Ensure lack of background mycoplasma as every lot is tested for undetectability of mycoplasma DNA
- Pair with the TaKaRa Mycoplasma qPCR Detection Kit for a complete mycoplasma testing solution
More Information
| Technology | Silica membrane |
| Format | Spin column |
| Sample material | Cells, cell culture supernatant, cell culture media, freezing media |
| Sample amount | 200 μl, pelleted from a 2 ml cell suspension |
| Fragment size | 100 bp–50 kb |
| Elution volume | 34 µl |
| Preparation time | 50 min |
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2025 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
