Isolation of genomic DNA from whole blood—NucleoMag Blood
NucleoMag Blood kits are designed for rapid, parallel purification of genomic DNA from whole blood in 96- or 24-well formats. After lysis of blood cells, genomic DNA is selectively bound to the NucleoMag B-Beads. Contaminants are washed away and the DNA can be eluted from the beads under low-salt conditions. Since the magnetic bead technology provides a closed system (no transfer of sample from one well to another is necessary during the preparation), the risk of cross-contamination is reduced.
NucleoMag Blood kits are designed for rapid, parallel purification of genomic DNA from whole blood in 96- or 24-well formats. After lysis of blood cells, genomic DNA is selectively bound to the NucleoMag B-Beads. Contaminants are washed away and the DNA can be eluted from the beads under low-salt conditions. Since the magnetic bead technology provides a closed system (no transfer of sample from one well to another is necessary during the preparation), the risk of cross-contamination is reduced. NucleoMag technology can be easily adapted to common liquid-handling instruments: it can be used either with static-pin separators (e.g., NucleoMag SEP) or magnetic separators integrated into robotic workstations. Purified DNA obtained with these kits is ready for use in PCR, Southern blotting, or any kind of enzymatic reaction.
Overview
- Single-tube procedure minimizes risk of cross-contamination
- Small elution volumes: ≥50 µl for NucleoMag Blood 200 µL, ≥1 ml for NucleoMag Blood 3 mL
- Complete processing is possible at room temperature
- Easily adapted to automated use
- Consistently high yields
More Information
| NucleoMag Blood 200 µL (Cat. # 744501.1 and 744501.4) |
NucleoMag Blood 3 mL (Cat. # 744502.1) |
|
| Technology | Magnetic bead technology | Magnetic bead technology |
| Format | Highly reactive superparamagnetic beads | Highly reactive superparamagnetic beads |
| Processing | Manual or automated | Automated* |
| Starting material | 200 µl whole blood (fresh or frozen, EDTA or citrate treated) | 3 ml whole blood (fresh or frozen, EDTA or citrate treated) |
| Fragment size | 300 bp to ~50 kb | 300 bp to ~50 kb |
| Typical yield | 2–8 µg (from 200 µl of blood) | 100–130 µg (from 3 ml of blood) |
| A260/280 | 1.6–1.9 | 1.6–1.9 |
| Elution volume | >50 µl | 1 ml |
| Preparation time | 45 min/96 preps* | 60 min/24 preps* |
| Binding capacity | Approx. 0.4 µg/µl | Approx. 0.4 µg/µl |
*Established on KingFisher Flex.
Applications
- Manual or automated isolation of genomic DNA from whole blood
Obtain DNA that is ready for:
- PCR
- Real-time PCR
- Southern blotting
- Enzymatic reactions
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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